Antibody Binding of Antigens

1217 Words Feb 20th, 2018 5 Pages
The antibodies are able to do so due to the binding of their antigen-binding domains to specific epitopes along an antigen’s surface. These epitopes can exist in linear, structural, and posttranslationally modified forms that vary between pathogens. The characterization of these epitopes is important because they can provide potential targets for vaccines. Thus, study of the interaction between antibody and epitope is needed. This will require the production of antibodies to test against a wide array of antigens; however, since the binding of epitopes is the main focus, only the Fab region of the antibody is required because it contains the antigen-binding region (Murphy 2011). Therefore, we seek to describe how to clone just the Fab fragments, characterize their functionality for binding, and map the epitopes they bind. In order to clone just the Fab fragments of antibodies, specific primers are required to direct cloning of the portions of the antibody heavy and light chains that coincide with the Fab region; however, before primers can be designed for such an experiment, it is important to have the DNA, or in this case cDNA, that is to be cloned. This…
Open Document