Anticancer Properties of Piper Betle Leaves Active Compounds

1607 WordsJun 20, 20187 Pages
Colorectal cancer is the third most common cancer in the world (World Cancer Research Fund International). Colostomy, chemotherapy, radiotherapy and biological therapy are among the most common treatment for colorectal cancer. These treatments may be used by itself or in combination. One of the most commonly used drugs for the treatment of colorectal cancer is 5-fluorouracil (5-FU). 5-FU is a cell cycle–specific, S-phase–dependent fluorinated pyrimidine analogue (Yim, et al., 2006). Due to its short biological half-life, it is always used in combination with other drugs to enhance its drug effect. However, toxicity and drug resistance often occurred in anticancer drugs combination therapy, thus, there are many ongoing research to look…show more content…
2011). Hence, in this study, we will test the effect of both PBL and active compounds combined 5FU on colon cancer cells. Although experiments have demonstrated the chemoprotective effect of PBL; however how it exerts its anticarcinogenic potential is not well understood. We proposed to use proteomic approach to determine the possible mechanism(s) of PBL active compounds in enhancing the anticancer effect of 5-FU. Proteomic approach is able to profile the overall protein expression in colon cancer cells during PBL -5-FU-induced cell death and will effectively reveal the possible mechanism(s) of action. 2. Objectives 2.1. To isolate the active compounds from PBL extract 2.2. To investigate the potential anticancer effect of the active compounds on HT29 colon cancer cells. 2.3. To investigate the effect of the combination of anticancer drug 5-fluorouracil and PB leaves active compounds on HT29. 2.4. To identify changes in protein expression stimulated by 5-fluorouracil and PB leaves active compounds combination treatment and investigate the possible mechanism of actions by using top down proteomics 3. Hypothesis Active component isolated from Piper betle leaves (PBL) can acts as an additive to 5-fluorouracil (5-FU) and enhance the cytotoxicity effect of anticancer drug for colon cancer. Proteomic

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