Antimicrobial Activity of Soil Isolates

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Antimicrobial Activity of Soil Isolates

Abstract: Isolation and characterization of microorganisms is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining Which is done for this project to enumeration which is quantitative description Based on the amount of microbial colonies available. The Antimicrobial Activity of three different microbes were also tested and results were obtained For two out of three of the microbes. Two out of three of the tested
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After initial isolation a variety of techniques can be implemented to analyze and characterize the various microorganisms. The Gram Stain is a procedure that is performed in order to determine the difference between two main microbial cell wall types. Gram-positive cells have a cell wall made up mostly of a thick, peptidylglycan layer. These cells retain thecrystal violet color and the acetone alcohol acts as a dehydrating agent causing all peptidoglycan pores toclose and preventing the insoluble Crystal violet-Grams iodine complex from decolorizing. These cells appear PURPLE.
Gram-negative cells losetheCrystal violet-Gram’s iodine complex easily because the acetone-alcohol cannot seal the thin peptidylglycan layer to stop the decolorization. These cells lose the complex, but take up the lipid soluble counterstain, Safranin. These cells appear RED/PINK(laboratory Manual). Observing the types of antibiotics that an organism produces is a tool which could help classify and identify a microorganism also. In contrast to the just mentioned point with the aid of antibacterial disk a researcher could identify what antibiotics hinder the growth of the bacteria could help identify the microorganism. Each of these methods may determine one or more aspects that maybe unique to
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