Antimicrobial Activity of Soil- Research Proposal Abstract

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ANTIMICROBIAL ACTIVITY OF SOIL BY SITUMBEKO LIWELEYA (s213459531) Research Proposal Submitted in fulfillment of the requirements for the degree of Bachelor of BIOMEDICAL TECHNOLOGY | | FACULTY OF HEALTH SCIENCES | at Nelson Mandela Metropolitan University | MODULE- RESEARCH METHODOLOGY, SUPERVISOR- PROFESSOR N. SMITH |

Contents ANTIMICROBIAL PROPERTIES OF SOIL 2 ABSTRACT 2 LITERATURE REVIEW 3 AIM OF RESEARCH 4 OBJECTIVES OF STUDY 4 RESEARCH METHODOLOGY 4  METHOD(S) TO FACILITATE MICROBIAL IDENTIFICATION 5 Gram stain 5 Culturing 5  MATERIALS TO BE USED 5  ETHICAL CONSIDERATIONS 6  SAMPLE COLLECTION 6  SAMPLE ANALYSIS METHOD 7 DAY 3 – Expected Results 9 DISSEMINATION OF
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Infections caused by resistant microorganisms often fail to respond to conventional treatment, resulting in prolonged illness and greater risk of death.
World Health Organisation (2012) discusses the problem of antimicrobial resistance
Synthesis of medicinally important compounds is very difficult and thus the cost of medicine is also high because of the non availability of source materials especially aromatic compounds
Gopalakrishnan (2011)
AIM OF RESEARCH * The main aim of this research is to determine whether or not soil bound microbes’ exhibit antibiotic activity against known bacteria of medical importance.

OBJECTIVES OF STUDY * To develop a new antimicrobial agent. * To reduce the ever expanding problem of antimicrobial resistance in treatment of bacterial infections.
RESEARCH METHODOLOGY * METHOD(S) TO FASCILITATE MICROBIAL IDENTIFICATION
Gram stain * Gram stain will be used to identify the gram bacterial membrane reaction for easy identification.
Culturing
* Nutrient agar will be used for the culturing of the soil supernatant to favour growth of most bacteria. * Muller-Hinton agar will be used for testing antimicrobial activity * Blood agar useful for pure culture isolation and determination of haemolysis with fastidious microorganisms.
Cheesbrough (2000)
Biochemical reactions
Biochemical reactions will be used for the

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