Antiseptic Lab Report

There are many differents ways to identify a bacterial unknown and many different situations where identification would be beneficial. One way to identify bacterial unknowns is to perform biochemical tests. In this experiment multiple biochemical tests were done, by performing these tests on the bacterial unknown received the two different bacteria were then identified. The citrate test is done to test the ability of organisms to use citrate as a carbon source. This test uses Simmons citrate agar, the agar contains sodium citrate as the only carbon source and has bromothymol blue as the pH indicator. The organisms that use citrate as a carbon source use the enzyme to transport the citrate into the cell. The cells converts ammonium dihydrogen

OBJECTIVE The objective for Rabie and Curtis (2006) was to determine the influence of hand washing on the risk of respiratory infection. METHOD The method adopted by Rabie and Curtis (2006) was to study a number of primary and review articles from five diverse databases before June 2004 in differing languages, to create a systematic review. Included in the review were studies which identified the impact of an intervention to promote hand cleansing on respiratory infections. Studies regarding hospital-acquired infections, long-term care facilities and the elderly were excluded. All studies were then evaluated where a conclusive decision was reached by consensus. Interestingly, from a primary list of 410 articles, only eight interventional studies reached the eligibility criteria. RESULTS The eight eligible studies disclosed that hand washing with antiseptic soap lowered risks of respiratory infection; the risk reduction identified as being from 6% to 44% and this range figures implied that hand washing can indeed reduce the risk of respiratory infection by 16% (Rabie and Curtis 2006). CONCLUSION Rabie and Curtis (2006) concluded that the studies collected were of insufficient quality and only one of the studies related to severe disease as well as none of the studies related

Dirty hands is the common source of spreading infection. It is very important to keep hands clean to avoid getting infected and spreading infection in the community. It is important to wash hands to keep hands clean. There are two ways to keep hand clean, one way is wash hands with soap and warm water while rubbing hands together for minimum 15 to 30 seconds. Indication of washing hands with soap and water is when hands are visibly dirty, before and after eating, feeding, using the toilet, after coughing or sneezing, after using gloves, taking care of patients. There is also second way to clean hands, but it is advisable to wash hands with soap and water all the time, but it can ignore when soap and water is not available so it is okay to use hand gel or foam in the form of sanitizer. This helps to clean hands or kill germs when hands are not visibly dirty.

Three paper towels A, B, and C where tested on absorbency. This experiment was tested twice. Paper towel A was 26.5 by 28 centimeters. Paper towel B was 26.5 by 28 centimeters. Paper towel C was 15 by 28 centimeters. Scissors were obtained and each paper towel was cut to 13.5 by 15 centimeters. The weight of the three paper towels at this size was 0.9 grams dry for paper towel A, paper towel B weighed 0.8 grams dry, and paper towel C weighed 1.2 grams. Three beakers weighing 12.2 grams each were obtained. Each beaker was filled with 100ml of water with the outcome of them weighing 128.1 grams each. The weight of each paper towel dry was deducted from the weight of each paper towel. Paper towel A was left to soak for seven seconds.

This experiment illustrates the importance of handwashing and proves that hand washing is worth it. Since our hands are constantly coming into contact with ourselves and others, touching surfaces, grabbing objects, being sneezed into, etc., keeping our hands clean is one of the most effective, yet simple way we can take to avoid getting sick and spreading germs to others. Many diseases and conditions are spread by not washing hands with soap and clean, running warm water. “The human skin is a host to anywhere between 10,000-10,000,000 bacteria per square centimeter and since health care providers come into contact with pathogenic bacteria by being engaged in patient care, hand washing can reduce the risk of spreading diseases (page 3).” The objective of the experiment is to test the effectiveness of hand washing and demonstrate normal flora. This report presents the procedures and materials for the experiment, the experiment's results, and an analysis of those results.

Hospital acquired infections are a big problem today, but the use of disinfectants by the medical staff can help to avoid or limit such exposures to pathogens. The authors’ main purpose of this research article was to educate and teach the importance of hand hygiene and test the reason why certain virucidal hand-rubs result in poor compliance due to the poor tolerability of the products. The overall goal with the study would to show the reason certain hand rubs are not being used and how to improve their formula to increase compliance. The article written by the author was very well written with a thorough abstract. The study was very easy to follow and read, due to the well thought out structure of the article. The authors introduction starts out by stating the approximate amount of hospital acquired infections in the European Union and why hand sanitizers play an important role to avoid such infections. The introductions statement showed the importance of the topic. Although some of the results were complicated and confusing, the discussion laid out the results in layman

This study was intended to prove that hand hygiene practiced according to the CDC guidelines will decrease the incidence of hospital acquired infections. This could not really be proved in this study since the hospitals were not able to maintain improvement in hand hygiene. Health care workers were familiar with guidelines but significant practice changes were not maintained. Some of the infection rates did improve during this time but the correlation with hand hygiene is not consistent. There were other practice changes occurring during this same time and those changes may be responsible for the decreased infection rates.

When investigating this experiment I found many different types of antiseptics all with different effects on bacteria and the effectiveness of all these antiseptics varied. I found

To help the prevention of infection spreading is by knowing the method of washing your hand because we carry most bacteria sue to the open air that we come in contact with. For example we use are hands to shake hands with someone holding or touching objects. When you are performing any form of hand hygiene you will need to make sure that you have washed your hand with anti-bacterial liquid soap; this is to help prevent any bacteria which we already have on our hands. In all health and social care settings an automatic liquid dispenser should be placed so that when washing hands any individual doesn’t touch or need to even sneeze any part of the liquid

In this project, C. Elegans are hermaphrodite worms that will be used since they are easy to maintain in lab, as well as have short life cycles. The gene that the project attempted to knockdown in C. Elegans with RNAi treatment is the unc-22 gene. RNAi disrupts gene expression in the presence of double stranded RNA (dsRNA) that is complementary to target gene sequence. The unc-22 gene codes for a muscle protein called twitchin in wild-type worms. The Unc-22 is required for muscle regulation and maintenance in C.Elegans. To verify that the RNAi treatment worked, would check the unc-22 mRNA levels in the worms, in addition to phenotype observation.

Consistence change is connected with lessened contamination rates and resistance spread. Hand rub application as indicated by prescribed practices is another option to ordinary surgical hand cleaning with disinfectant cleanser and water for surgical hand readiness. Therefore for the hospital to prevent infections and decrease infection rates, a system change will be essential to change a hand hygiene agent, encourage and facilitate skin care, and introduce monitoring and regular performance feedback of hand hygiene compliance.

The skin is one of the most important organs of the whole human body. It is the largest organ in the human body and contains various functions including protecting the body from injury and preventing fluids from leaving or entering the body (Boer et al. 2016). In animals, the skin has various functions as well. The skin aids with locomotion in bats and aquatic mammals, help camouflage the body in chameleons, helps with finding mates for deer with their white tail, and serves for many other purposes. The skin is composed of three layers in the human body: the epidermis, dermis, and subcutaneous layer. Melanin is produced from melanocytes in the epidermis of the skin. Melanin is important for protection from ultra-violet (UV) rays.

* Hand washing is the most important method of preventing the spread of infection by contact (Ayliffe et al 1999). The Nottingham University Trust Policy on Hand Hygiene (2009) states that there are three types of hand hygiene, the first is ‘routine hand hygiene’ which involves the use of soap and water for 15 – 20 seconds or the application of alcohol hand rub until the hand are dry. The second is ‘hand disinfection’ which should be used prior to an aseptic procedure by washing with soap and water and applying alcohol hand rub afterwards. The third is ‘surgical hand washing’ which is the application of a microbial agent to the hands and wrists for two minutes. In addition to which a sterile, disposable brush may be used for the first surgical hand wash of the day although continued use will encourage colonisation of microbes. The third example is the most appropriate to any O.D.P undertaking the surgical role as it is the best way for the surgical team to eliminate transient flora and reduce resident skin flora (World Health Organization 2010). The first and second are important to any O.D.P undertaking any other role within the Operating Department as this is the best way to reduce the transient microbial flora without necessarily affecting the resident skin flora

Life on this planet began with microorganisms. Through millions of years microorganisms have found ways to successfully adapt and survive. These adaptations have created a wide biodiversity, allowing them to basically populate in all places. Why are these microbes so important? Because they shape the history of our world. Some microbes can be deathly to humans while some others are favorable, for example, bacteria that lives in the gut of both humans and animals and helps during the process of digestion (Alfred Brown & Heidi Smith, 2006). Understanding these interactions help scientists to find ways to protect humans from potential deathly pathogens. In order to observe microbes, microscope proficiency and microorganisms’ identification are crucial skills in a microbiology lab. During this laboratory session, samples of environmental and human organisms were inoculated into two different rich media and incubated to their according temperature. After this, appropriate use and calibration of the microscope was performed. Lastly, morphology and size of different species of bacteria, algae, fungi and protozoan were recorded.

This assignment will encompass how the results of the personal hygiene and susceptibility microbiology experiments provide a framework for the basis of the NICE Clinical Guidance (CG139) on Infection and how crucial the reasons for the hand wash protocol and hygiene is in all healthcare settings.