Ap Bio Lab Report

Directions: Design your own experiment to test some variable of the Diet Coke and Mentos lab. Identify one variable that you will change and test – this will be your Independent Variable.

Firstly acquire three test tubes. All test tubes will contain gelatin. One of them will contain both pineapple and compound X. Compound X is our independent variable in this experiment. Another will contain just pineapple and gelatin. The last one will contain only gelatin, this test tube will be our control group. The next step is to put all the test tubes in the icebox and wait for the gelatin

The materials used for the experiment were 800 ml glass jars, two pieces of cotton string that both measured 6.5 inches long, granulated white sugar, 4 cups of water, two wooden chop sticks, parchment paper, two small same size, shape, and type metal screws, tablespoon measuring spoon, permanent marker, ruler, tape, pot, stove,

Students measure out 5mL of the colored beverage. Next, students measure out 50mL of the food dye stock that was in the chosen beverage. Students recorded the concentration of the food dye that was labeled on the bottle. Then, students obtained 5 test tubes and labeled them 1-5. Using a pipet, students added different ratios of food dye solution and distilled water in each of the test tubes.

In conclusion, I have acquired a new knowledge about how to test for macromolecules in food such as carbohydrates, starch, proteins, and lipids. To begin with, I knew that potato do have sugar in them so it will definitely have positive reaction and I was right about that. When you add Benedict's solution to a food that contains sugar, the food will change its colour from blue to green to reddish brown and the same thing happened to potato. Furthermore, the sucrose solution and distilled water contains starch because when drops of iodine were added into these liquids they changed their colour into yellowish-brown which indicates the sign of the starch in these substances. In addition, an egg albumin solution and milk contains protein in them

Then carefully draw 4 ml of yeast from the beaker using the pipette and into the test tubes, while doing this make sure to do one test tube at a time and leave the other test tubes in the test tubes holder to avoid spills. Next add 5 ml of water to test tube 1 using the pipette, which already contain 4 ml of yeast. Then put the test tube back into the test tubes holder and pick up test tubes number 2 and add 5 ml of glucose to test tube 2. Now for the test tube 3 fill it with 5 ml of protein solution. Once you have filled all three test tube with 4 ml of yeast and one of each solution in different test tubes, place them all back onto the test tubes holder. Now to construct a simple respirometer, lower a large test tube down over one of the 10 ml test tube as far as it will go. Invert the tubes quickly, so that very little

4.Mix each tube, then take out 1 drop of each tube with a coffee stick and place on a glucose test strip. wait 30 seconds, and read test strip using color scale. Record Data.

Marked a line on the test tube at the liquid level, repeated this step for test tubes 1,3,5,7,9 and 11.

Set up solutions in the medicine cups: labeled one of the medicine cups Patient #1. Used graduated cylinder to measure 5 mL of Patient #1 intestinal fluid and poured it into the Patient #1 cup. Labeled one of the medicine cups Patient #2. Used graduated cylinder to measure 5 mL of Patient #2 intestinal fluid and poured it into the Patient #2 cup. Labeled one of the medicine cups Negative Control. Used graduated cylinder to measure 5 mL of Negative Control intestinal fluid and poured it into the Negative Control cup. Labeled one of the medicine cups Patient #4. Used graduated cylinder to measure 5 mL of Positive Control intestinal fluid and poured it into the Positive Control cup. Labeled one medicine cup “Milk” and added 25 mL of milk. Six glucose test strips tested baseline glucose labels of the intestinal fluids of the four patients and the two controls. Dipped the test area of the glucose test strip into the Patient #1 cup and removed immediately. Strip was placed on the Glucose Test Strips Chart on the section labeled Patient #1, 0min. After 30 seconds, the color of the test area was matched to the glucose color chart and recorded on the

A large beaker was filled to the top and a 20ml syringe was immerse until all air was released, attach a clamp. 1 ml of yeast was put into a boiling tube using a pipette then a bung was put in the top with a tube and a 5ml syringe attached. The 5 ml syringe had different measures of pH concentrations. When the pH was inserted into the boiling tube a timer was started and the reaction was recorded in a table. Each retest was done in the same way.

Number test tubes 1-4. Add 30 drops of each saccharide solution (monosaccharide solution in 1, disaccharide solution in 2, polysaccharide solution in 3, unknown solution in 4).

Lugol’s test was utilized to test for polysaccharides. Starch turned black and the negative control of water stayed brown, the color of the iodine being used to test (Lugol’s test image 1). Maltose, sucrose and lactose all tested negative. However, glucose tested positive. This was an error because glucose is a monosaccharide. The test was repeated and it was found that the test tube had not been properly cleaned and there was leftover starch in it. The test was repeated and glucose tested negative this time. These results are displayed in Results of Lugol’s test for known carbohydrates table. This result supported the hypothesis that glucose is a monosaccharide not a polysaccharide. Only starch tests positive because it has a helical shape while the rest are

We placed the 2 plastic centrifuges in the centrifuge, which spun the solution for 3 minutes at 6000 rotations per minute (rpm). We then poured the upper layer liquid (supernatant) from both the plastic centrifuges into a clean beaker, leaving the white pellet in the plastic centrifuges. Then, we removed a small amount of the solution from the beaker, and placed it in the spot plate, adding a drop of iodine. By adding the iodine, it would tell me if the starch was present or not. Given that the solution did not turn blue or black, we can say that the solution tested negative, indicating starch was not present. We then put the test tube E’s in their perspective hot water bath, one in each of the following; 40℃, 60℃, and 80℃ for about 10 minutes. After taking out all of our test tube E’s, we added 30 drops of starch-free enzyme extract (the solution that we just tested), from the beaker to all of the test tubes (all 8). We mixed all of the solutions by hand, where we then added a small drop of soluble starch to each test tube, that we also mixed by hand. Next, to test the solutions/contents, to see if starch was present, set up my spot plate so that it would be organized to conduct the experiment. We did this by taking tape and labeling the left side of the spot plate, with letters of the test tubes that would be tested. Above the spot plate we laid a piece of tape along the top of the spot plate, labeling with the amount of time that

There are 6 main nutrients present in the food such as carbohydrates, protein, fats, vitamins, minerals and water. But according to chemistry, foods can be classified as micro nutrients (vitamins and minerals-required in small amount) and macro nutrients (carbohydrates, proteins and fats-required in higher amount). Carbohydrates can be classified into two different classifications as sugars (monosaccharides and oligosaccharides) and non-sugars or polysaccharides according to its chemical structure. The whole classification of carbohydrates can be classified as follows.

The second lab manages the chromatography of nourishment colors which implies you make sense of what sort of mixes are utilized as a part of an item like sustenance comparing so as to shade and so forth the outcome to FD&C Food Dyes. You utilize six diverse nourishment colors, four sustenance hues out of the store, two distinctive Kool Aid powder and four unique shades of sweet like M&Ms you use for the chromatograph. Toward the end you attempt to discover which nourishment colors are in alternate items. Test and Observation