Ap Biology Lab

They are different, because you can see so much more when you look through a microscope. For instance, you can see a strand of hair through a camera, at a tenth of a millimeter, but not a cell, at 100th of a millimeter.

The major storage polysaccharide in plants is starch. These molecules would be found in abundance in the stroma in the plant tubers where it is found as granules. Glucose is stored mainly in the form of starch granules, in plastids like chloroplasts and amyloplasts. Plant starch starts out as glucose, but glucose is very hard for plants to store, so it is converted to starch through polymerization. Amyoplasts turn the glucose into starch and move it to the stroma, and in tubers the stroma is a place to store the food (starch), and when plants need the energy in the starch, it converts the starch back into glucose.

First; a brief introduction about the confocal microscopy, critical aspects of confocal Microscopy, and the basic parts of the confocal microscope. It will follow by more information about fluorescence microscopy which is used for study fixed and living cells because of its versatility, specificity, and high sensitivity.

To understand the greatness of the microscope, the history of the microscope should be made known. The origins of the microscope can be traced back to the Roman invention of glass in the first century A.D. When the Romans experimented with different shapes of glass, they discovered that when the middle of the glass was thicker than the edges, things that were seen through the glass appeared larger. This discovery evolved into the first eye glass, which was made in the thirteenth century. The first true microscope was first invented in 1590 by the Dutch glasses makers Zacharias Janssen and his father, Hans. The first person to build and actually use the microscope scientifically was Anton van Leeuwenhoek in the seventeenth century. He achieved much greater magnification than other scientists had, and because of this, he was able to be the first to see miniscule creatures such as bacteria and microorganisms living in a drop of water.

Using the microscope provided to analyze the different size of the different cells and organisms

The investigator will examine duct tape piece “A” against duct tape pieces “B”, “C”, and “D” to determine similarities and differences between them utilizing increasingly strong microscopy methods—beginning with the unaided eye, then utilizing a magnifying glass, and ultimately a stereomicroscope. While using each of the above methods, the investigator will carefully note observations made with each in his or her lab notebook. These observations will come in the

Light source: it also known as illuminator. Usually it is situated at the base of the microscope. Used to provide light to the sample in order to facilitate the experiment.

“The short (about 1 millimeter) focal lengths of the lenses would have necessitated placing the eye almost in contact with the lens” ("Anton van Leeuwenhoek"). Leeuwenhoek obtained the clear image by carefully moving the angle of lighting left and right. Leeuwenhoek's techniques of lighting samples under the microscope are still not well known today. This was the only secret that he took to his grave. Even though the simple microscope was difficult to use, scholars visited Leeuwenhoek to be educated on his design. Leeuwenhoek went all over the world giving demonstrations about his microscope for high-ranking people. Without Leeuwenhoek’s simple microscope, microbiology today would not be as advanced.

pathologist to zoom in on different parts of a slide and pan around the slide on the

2.What type of microscope would you use to investigate each of the following scenarios and why (your answer to question 1 will help):

The oldest actual microscope was actually just a tube with a plate at one end and a glass lens at the other end. They magnified

Most modern day microscopes used in labs outside of the school uses electricity and use light to help function the microscope. A scientist in Canada actually used an iPhone to see through a woman’s intestine.

The microscope has been one of the greatest contributions to scientific study known to mankind and like a hammer is found in every carpenter 's toolbox, one would find a microscope in a scientist 's toolbox. A microscope is used to view matter not obvious with 20/20 vision and can magnify objects as small as the smallest atom. The nomenclature for microscope derives from two words, mikros and skopein which means seeing small. The history of the microscope, which is comprised of high powered lenses, dates as far back as the year 100 when Romans looked through glass they found and realized objects appeared larger than actual size. The glass used in the modern day microscopes called lenses from the Latin word “lentil” is the primary component of any microscope (Sashmasusik & Hayath, 2015).

The modern light microscopes is made up of more than one glass lense in combination. When an object is placed at the focus of convex lense , its magnified, inverted and real image is obtained.

The data were obtained by Leica Qwin 500 image analyzer computer system (England).The image analyzer consisted of a colored video camera, colored monitor, disc of IMB personal computer connected to the microscope and controlled by Leica Qwin 500 software. The image analyzer was first calibrated automatically to convert the measurement units (pixels) produced by the image analyzer program into actual micrometer units. Using the measuring field menu the area, area % and standard measuring frame. Measurements were done using an objective lens.Ten readings were obtained in each specimen.