Artemia Franciscana Lab Report

Brine Shrimp are a micro-crustacean that is found mostly in inland saltwater bodies, however, the salinity levels that they live in vary greatly. The conditions that they require can be made at home as the eggs (cysts) of the shrimp are dry, hard shells that can withstand drought and go without water for up to 50 years. This study presents the results of 3 different salinity levelled environments to the Brine shrimp in order to discover a salinity level that produces the most successful hatching rate. By filling 3 Petri dishes with 20mL’s of purified water, each dish contained varying sodium chloride amounts from 15ppt, 25ppt, and

Table 2: Mann-Whitney U statistical testing for the difference in average heart rate of Daphnia before and after the addition of aged water:

The purpose of this lab was to answer the question, “Of the saline solutions of 0%, 0.5%, 1%, 1.5%, and 2% sodium chloride (NaCl), which solution will yield the highest hatching viability for the brine shrimp?” The hypothesis was that the saline solution most precise to the concentration of NaCl in seawater, which is approximately 3.5%, will yield the highest hatching viability. Therefore, the 2% NaCl solution will yield the highest hatching viability.

Crayfish, Orconectes propinquus, are ectotherms that do not generate internal heat and rely on external temperatures to determine their body temperature. The crayfish we tested were found in Medway Creek and therefore undergo considerable temperature changes throughout the year. Since these crayfish are able to adapt to various temperatures allowing them to live in numerous places, their survival rate would be higher than many other organisms.

The Artemia franciscana can survive in extreme conditions of salinity, water depth, and temperature (Biology 108 laboratory manual, 2010), but do A. franciscana prefer these conditions or do they simply cope with their surroundings? This experiment explored the extent of the A. franciscanas preference towards three major stimuli: light, temperature, and acidity. A. franciscana are able to endure extreme temperature ranges from 6 ̊ C to 40 ̊ C, however since their optimal temperature for breeding is about room temperature it can be inferred that the A. franciscana will prefer this over other temperatures (Al Dhaheri and Drew, 2003). This is much the same in regards to acidity as Artemia franciscana, in general thrive in

At the start of this experiment we were required to obtain a set of four Wisconsin Fast Plants, which are genetically, known as Brassica rapa. These plants have been, “originally selected under continuous fluorescent light to grow and reproduce quickly for research purposes, these petite, fast-growing plants have been used for teaching biology concepts” (Wisconsin Fast Plants). These four pots that contain our plants will be under our watch for the next 16 weeks where we will show our results at the end of the semester.

Behavior of Brine Shrimp in Habitat Selection Introduction: This experiment was developed primarily to find the optimal or preferred living conditions of adult brine shrimp. In this lab 3 variables were tested in different degrees to determine which the shrimp prefer. The different variables were light, ph, and temperature. From outside resources I have found that brine shrimp can survive in temperatures ranging from 4° Celsius to 40° Celsius with an optimal temperature of 20°-25°. So I hope to reinforce this research by testing its validity in the lab. From another outside source brine shrimp display positive photo taxis, which means that they are attracted to light. And finally from my last outside source I have found information that

To perform this test, a tube of broth rich with glucose is acquired. In this tube is phenol red, a pH indicator. Initially, the tube appeared pink in color, indicating a normal pH level. Next, a sample of unknown #44 is introduced into this medium using the aseptic technique, and this is allowed to sit for several days. If the organism is able to ferment glucose, the pH in the medium would decrease and cause the phenol red to exhibit a yellow color. In addition to the straw color, gas can also be produced and trapped inside the Durham tube placed in the medium. This production of acid and gas is a direct result of the fermentation of glucose, as seen with unknown

The lobsters which were put in tanks of varying salinity adjusted their hemolymph osmolarity to that of the external environment, making Homarus americanus an osmoconformer. The tank which had the lowest salinity

For an existing habitat, an increase in salt levels will begin to show an effect on the abundance and diversity in species of zooplankton. This will exhibit a detrimental effect on the daphnia from the limited osmoregulatory capabilities of the organisms. This in turn can pose a risk to the level feeding rates and survival (Heine-Fuster, Vega-Retter, Sabat & Ramos-Jiliberto,

Different aquatic invertebrates live in different micro habitats (smaller habitats) at Lake Tonetta. Some live on the surface of the water. Others live in the bottom of the lake, or deep within the sediments at the bottom of the lake. The water pH is important because they only can live in a specific pH 6.5-7.5.When we visit the lake our group checked the biodiversity (The variety of plants and animal species in an environment)of Lake Tonetta by counting the aquatic invertebrates in a sample obtained using dip nets and bottom scrapers. During our trip, we found beetles, snails, worms ,

Artemia salina are used as environmental indicator species. They are used to test changes in their environment. A. salina is sensitive to toxins, temperature, and drugs. For the experiment, cysts were put into different amounts of ethanol alcohol and their viability was measured. The hypothesis used was if there is more ethanol alcohol, then the viability of the brine shrimp is unfavorable. The brine shrimp were put into sixteen Petri dishes with the same amount of brine solution. Different amounts of ethanol alcohol were added to each Petri dish. After 168 hours, the brine shrimp were

The population dynamics of Daphnia magna are observed under three different conditions; low, medium, and high density. The effects of different population densities on the survivorship and reproduction of Daphnia are observed over a two-week period within a lab environment. Over the two week period, the numbers of parent Daphnia alive and dead are recorded daily, along with the amount of offspring produced each day. From the main parameter investigated, the net reproductive rate, the results of the experiment support that higher densities result in less successful reproduction and decreased fecundity. Values for the instantaneous growth rate of the populations also suggests that low and medium density populations allow for

et al. 2014). Shell dissolution due to ocean acidification has been observed in several species of pteropods (Johnson K.M. et al 2016; Bednaršek N. et al 2014; Busch D.S. et al. 2014). Lischka S. et al (2012) reported that the aragonite saturation state <0.3 resulted in the reduced survival rate in pteropods. Along with survival, growth and a biochemical functions impairment also reported due the negative effect of ocean acidification (Lischka S. et al. 2011; Hoshijima U. et al 2017).

An investigation into the effects of varying seawater concentrations on two marine invertebrates’ osmoregulatory abilities; Carcinus maenas and Arenicola marina.