Essay on Aseptic Technique & Culturing Microbes

14) [2.2] If a nutrient is limited for a particular species, what happens when you add more of that nutrient to the

7- what term refer to allowing food to remain for too ong a time in a temperature that allows the growth of germs

9. After the 48 hour time period the inhibition of the Escherichia coli bacteria around the disinfectant disks was measured. A ruler was used to measure the clearing from the edge of the disk to the perimeter of the clearing. Irregularities in the clearing were not included in the area of clearing.

The conditions needed for the growth of micro-organisms are: Micro - organisms need food to survive. They like high protein food to survive, eg. Poultry & fish. Most micro - organisms need warmth & grow best at 20-40c. They need moisture to multiply. They need air to multiply, though some can without. A single Micro-organism becomes two every twenty minutes.

Most micro-organisms need most of the things you and I need to flourish and grow - moisture, warmth and a source of nutrition - some (usually the most dangerous such as those responsible for some types of gangrene) can do without oxygen (anaerobic bacteria). They need a mode of spread (usually the unwashed or poorly washed hands of people or badly cleaned equipment/facilities or badly stored food) and a vulnerable person to invade.

To maintain life, organisms must be able to convert energy from one form to another. For example, in the process of photosynthesis, algae, plants, and photosynthetic prokaryotes use the energy from sunlight to convert carbon dioxide and water to glucose and oxygen (a waste product).

An Aerotolerant Anaerobe- can grow in oxygen if present, but does not use oxygen. If it was in a test tube there would be equal growth throughout the broth, While a Facilitated Anaerobe uses oxygen, it can grow without it. In a test tube there would be more growth at the top of the broth that anywhere else.

Controlling the growth of microorganisms is not always necessary, but when they become a danger to society there is a need. There are several effective ways that are used to either control or destroy the microorganism. Bactericidal refers to the killing of microorganisms and bacteriostatic refers to inhibiting the growth. Heat is most often used for killing microorganisms. Some examples of heat would be incineration which is used for needles, inoculating wires and glassware. Boiling is used for to kill endospores and sterilize a solution. Autoclaves use pressurized steam which can denature or destroy heat labile substances, but is good for sterilizing almost everything. A hot air oven ( dry heat) is used for glassware, metal and objects that do not melt. Hospitals use autoclaves to clean surgical instruments and anything that would be used for multiple patients. Some items are made for one time uses, such as for a patient that is isolation for an infectious microorganisms that

The priority nursing diagnosis of hospital acquired infection is risk for any kind of infection. One of the main goals for each patient in the hospital is the patient will remain free of infection as evidence by absence of heat, pain, redness, or swelling in any area of the patient’s body during each nurse’s shift. (care plan book). Frequently hand washing is the best intervention for preventing infection. Hand washing reduces the risk of transmission of pathogens by inhibiting the growth of or killing the microorganisms. (cb)Proper sterile technique during urinary

The energy system that is able to generate ATP without oxygen is called the anaerobic system. Anaerobic meaning without oxygen. This system is formed from the combination of ATP and lactic acid. The metabolic pathway of the anaerobic system is called anaerobic glycolysis. Glucose is broken down during the metabolic pathway glycolysis, with or without oxygen present. Carbohydrates are the only macronutrient that can be catabolized during glycolysis.

A few different factors that affect bacterial growth are the availability of resources and nutrients, temperature and pH. (Act For Libraries) stated in the above paragraph, once the resources and nutrients are

All living organisms need the energy to perform the basic life functions. Cells use a process called cellular respiration to obtain the energy needed. In cellular respiration, cells convert energy molecules like starch or glucose into a cellular energy called Adenosine triphosphate(ATP). There are two types of cellular respiration which include: Aerobic and Anaerobic respiration. In aerobic respiration, cells will break down glucose to release a maximum amount of ATP this takes place in the presence of oxygen. Aerobic also produces carbon dioxide and water as waste products and it takes place in the mitochondria. on the other hand, anaerobic respiration, a metabolic process, also produces energy and uses glucose, but it releases less energy and does not require the

Most life on earth is fueled directly or indirectly by sunlight. There are, however, certain groups of bacteria, referred to as chemosynthetic autotrophs, that are fueled not by the sun but by the oxidation of simple inorganic chemicals, such as sulfates or ammonia. Chemosynthesis is a process by which some organisms use chemical energy instead of light energy to make energy-storing carbon-based molecules. Chemosynthetic autotrophs are a necessary part of the nitrogen cycle. Some groups of these bacteria are well suited to conditions that would have existed on the earth billions of years ago, leading some to postulate that these are living representatives of the earliest life on earth. This view has been supported by the discovery of small

A bacterial growth curve illustrates a lag phase, a log phase, a stationary phase, and a death phase. The lag phase of the growth curve depicts no growth of the population. There is no growth because the bacteria cells are not undergoing division because the bacteria is still adjusting to the environment. The log phase depicts the period of time

In microbiology and population biology, the laboratory device chemostat extensively uses as a research technique to culture microorganisms continuously under nutrient limitation in a controlled environment in order to study the general