Essay Aseptic Technique and Bacterial Anatomy and Morphology

2. Used a test tube clamp to held the test tube and gently heated the tube in a laboratory burner flame for about 30 seconds.

The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible

Turn on the gas burner and bring it a low heat, only to the point that there is a blue flame. Carefully push the gas burner under the crucible and heat for around two minutes in order to properly rid the crucible of any bacterias or other impurities that may affect the lab.

9) Trial E: Remove the syringe and empty the beaker. Add a Thermometer to the beaker. Add 200 mL of Room Temperature water to the beaker and heat with a Bunsen Burner until it reaches 100° C. Remove the Bunsen Burner. Repeat Steps 5 & 6.

Use a test tube holder to put the test tube into a container of boiling water for 5 minutes, or until the solution changes color.

Two smears of the unknown bacterium #5 were inoculated while the second smear was used for a back up. The unknown bacterium dried for at least forty minutes. After the smears dried, the slides were heat fixed two times to ensure the stability of the organism. The slide was placed on top of the staining rack then over the small sink.

14. Use the same loop and technique to spread the same cell suspension (+) on the LB+AMP agar plates. Dispose of the sterile loop in a beaker of germicide.

There are many reasons for identifying an unknown bacterium. The purpose of this exercise was to identify an unknown bacterium from a liquid culture. We chose our unknown bacteria from a rack of test tubes with several different species of bacteria inside. I wanted to pick an unknown bacteria with a number easy to remember so I pick the test tube labeled “745”. Procedures were followed as stated in the lab manual written by Dr. Pedro J.A. Gutierrez.

4.Measure 35mL of warm water and add them into each of the 4 test tubes at about roughly the same time. It is essential that the water is warm. Do not seal the test tube.

When reflecting back to experiment 3, Aseptic Technique and Culturing Microbes, I realized the large amount of microorganisms that can be found in everyday life. Many different types are found with in the human body. Theses experiments focused on two types of bacteria. First was Staphylococcus epidermidis, found on the skin, and second was Lactobacillus acidophilus, found in the gastrointestinal tract. Both have similar needs for growth when it comes to temperature, however, different growth environments are used.

1 ml of water should be added to the first test tube and make a note. In the second test tube, 1 ml of methyl alcohol should be added. In the third test tube, 1 ml of hexane must be added. Lastly, the fourth test tube will be a control.

Check whether it is ready or not, and then open the burette to start neutralize the acetic acid. (Check the progress by change in colour)

Introduction: Through the conduction of numerous experiments, the identity of two bacterial isolates was determined. The tested specimen was an unknown sample of a mixed culture of two different species of bacteria. The first step that was taken was obtaining a pure culture of each species of bacteria by isolating one species from the other. Once isolation was complete, the isolated cultures were tested using procedures that had been performed during previous lab sessions. A gram stain was performed on the two isolates. The isolate which had tested gram negative was then tested for the presence of cytochrome C and lactose fermentation. For the gram positive isolate, cell shape was determined and a catalase test was performed.

1. Number four clean test tubes 1-4. 2. Place 1 ml (or 20 drops) of the following solutions into each tube: water, egg albumin, starch, and chicken broth. 3. Add 3 drops of Buiret reagent to each tube and gently shake

8.After 5 minutes, remove test tubes from the water bath set in test tube holder and record results. Also turn off hot plate.