Yeast, Saccharomyces cerevisiae strain D7, is a unicellular, eukaryotic organism. The rapid growth rate of S. cerevisiae allows it to be used to test for mutagens. The mutagenesis of S. cerevisiae can lead to visually observable alterations in its phenotypes when grown on different agar media.
Aspartame is a synthetic compound that is 200 times sweeter than sugar but contains no calories. Hence Aspartame has been used as an artificial low-calorie sweeteners and can be found in over 6,000 food items, and millions of Americans consume aspartame everyday (Lindseth et al., 2014 ). Despite its widespread use, aspartame remains one of the most controversial food additives (Magnuson, 2010).
The role of sweeteners in cancer risk has been widely
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In their experiments, they used aspartame in concentrations of 1.25ppm, 2.5ppm and 5ppm. After preparing the test-tubes, they incubated the test-tubes at 37 degree Celsius for 3 hours. However, as the experiment was involved human blood, 37 degree Celsius is the ideal temperature. For our experiment, we would keep the treatment of the yeast cells with Redoxon Double Action Vitamin C solution at 30 minutes in view of time constraints. This is also because Saccharomyces cerevisiae yeast strains have the fastest initial growth at near 30 degree Celsius (Walsh and Martin,1977).
Materials:
1. D7 yeast strain (ATCC 201137)
2. Ethylmethane sulfonate (EMS, a mutagen for positive control)(prepared)
3. Sterile water (for negative control)(prepared)
4. Medium plates (3 of each of the following): i. Yeast extract peptone dextrose (YPD) agar plate, ii. Agar plate containing synthetic medium lacking isoleucine, iii. Agar plate containing synthetic medium lacking tryptophan
5. Phosphate-buffered saline (PBS), pH 7.4
6. Water bath at 28°C
7. Distilled water
8. Redoxon© Double Action Vitamin C tablets
Procedures:
1. Resuspend yeast cells in PBS at pH7.4. (around 1 x 105 cells per
1) The substrate for this procedure was hydrogen peroxide. The enzyme used (contained in the yeast) would have most likely been catalase.
Numerous neurological and behavioral side effects have been linked with consuming aspartame at well below the approved safe limits set by the Food and Drug Administration (FDA). Aspartame is an artificial sweetener that has been ingested in the United States for over three decades. Even though there are several studies showing the harmful effects of its consumption. When aspartame is digested several toxic chemicals are produced, most notability formic acid & glutamate. Both of these chemicals cause the body to mimic the side effects of multiple sclerosis and Alzheimer. Even the passing of aspartame to be allowed in food was controversial. Just as the studies on it, continue to be today.
Higher levels of solution should produce higher levels of product. The independent variable for the control group data and the experiment data is the yeast concentration. The dependent variable for the control group data and the experiment data is how much oxygen is produced. The Constant for the control group data and the experiment data is time and amount of hydrogen peroxide. The products of the experiment will increase if the levels of reactants increase. Denatured yeast may cause change in the reaction of the experiment. For all trials of the control group, the concentration of yeast is 6ml. For the experiment data, the yeast concentration varies from 8mL, 10 mL, 12 mL, 14 mL, and 16 mL. The temperature may cause change in the reaction of the combination of yeast and hydrogen peroxide
In part II of the lab six small glass tubes were obtained in a test tube rack. Ten drops of distilled water were then added to test tube 1, five drops to tubes 2-4, and no drops in tubes 5 and 6. Five drops of 0.1M HCl were added to test tube 5 and five drops of 0.1M NaOH to test tube 6. Five drops of enzyme were then added to all tubes except tube 1. Tube 3 was then placed in the ice bucket and tube 4 was placed in the hot bucket at 80-900C for five minutes, the remaining tubes were left in the test tube rack. After the five minutes five drops of 1% starch was added to every tube and left to sit for ten minutes. After ten minutes five drops of DNSA were then added to all the tubes. All the tubes were then taken and placed in the
Before plating the strains on agar plates, dilutions of the three strains of cells were prepared with LB broth.
ban", ("Tufts University Health",2015). The food and drug administration is a federal part of the United states human and health services and they continuously promote the use of aspartame and have stated that there is “no new credible scientific evidence to change the agency’s position that the zero-calorie sweetener is safe for the general population", ("Tufts University Health",2015). Even a study stated in the article by the American cancer society “found no link between intake of diet sodas (most commonly sweetened by aspartame)
Who uses the sweetener or consume diet drinks may have heard of the controversy surrounding aspartame. The product has been linked with diseases or disorders such as migraine, multiple sclerosis, Alzheimer, cancer, lymphomas, and brain tumors, among others. This association is mainly caused by the fact metabolized in the human body-releasing methanol, a chemical compound that has toxic and accumulative effect on the nervous system. Despite being naturally present in small amounts in chicken in the meat, the beans and skim milk when ingested in large quantities methanol can cause blindness, among other problems. This fact has been known since the approval of aspartame in the United States, the Food and Drug Administration (FDA). However,
The purpose of this experiment was to test the effects that temperature, pH, and substrate
Vogue, the most popular fashion magazine has a huge impact on the way in which the public receives circulated information, thus contributing a huge aspect to the mass media. Images of women throughout the mass media provide society with false representations of females, which leads to the formation of stereotypes. Women are vulnerable to objectification and sexualisation due to the fact the media often portrays women to be nothing more than sex objects, solely existing to cater to the needs of men. In this essay I will be discussing Vogue’s March 2010 cover edition features supermodel Rosie Huntington Whiteley and the way in which it sexualises her in order to increase sale.
In this experiment the four different types of sugar substitutes will be tested with yeast to determine if the type of sugar substitute directly affects the respiration rate of yeast. The four different types of sugar substitutes include Saccharin (Sweet ‘N Low),
A sterile pipette was used to add 0.1ml of E. coli culture to the pH 3.0 tube. This was then repeated for the tubes at pH 7.0 and pH 9.The tubes were then incubated at 37oC for 48 hours. This was then repeated for saline culture of Saccharomyces cerevisiae but incubated for 72 hours at 25oC.
Advertisement and commercial industries portray table sugar, and other artificial sweeteners as fuel needed by the body. Though body and body cells need sugar as fuel source. The body mechanism is just like the case of engine, there are bad fuels which can destroy engine. Table sugar is just a bad fuel for the body. Several research studies have arraigned sugar first culprit for the epidemics of complicated health problems such as diabetes, obesity, heart disease and cancer. Worst of it, several research now ranking sugar as addictive substance as drugs.
There has been controversy over whether or not the United States’ elections are rigged; this theory came up again when Donald Trump claimed that John Podesta, a chairman on Hillary Clinton’s campaign, rigged the polls as a voter suppression technique on October 24, 2016 (Qiu). This isn’t the first time the elections authenticity has been questioned; when we think “rigged,” it is normally referring to accusations of direct voter fraud or in which the results were procedurally contested, extensively protested, or recognized as fraudulent by a reliable international organization (Wikipedia Contributors). In fact, in the past the polls have been rigged. There have been multiple cases in the United States where the presidential election was under scrutiny dating back to 1793 all the way up to 2004 (Wikipedia Contributors). Despite Trump’s claim and the couple incident where it has happened, it is pretty impossible to actually “rig” an election with voter fraud. But, this does not mean that an election has never been rigged; they are rigged to an extent but not how Trump claimed.
“Artificial Sweeteners: Sugar-Free, but at What Cost?” Harvard Health Blog, Harvard Medical School, 12 Dec. 2016, www.health.harvard.edu/blog/artificial-sweeteners-sugar-free-but-at-what-cost-201207165030. Accessed 28 Feb. 2017. Holly Strawbridge is an author for Harvard Health Blog. Strawbridge’s purpose in writing this article was to inform the general public of the dangers of artificial sweeteners. The article mainly briefs over the negative effects of consuming artificial sweeteners, specifically the diseases potentially caused by them. This article speaks more negatively of artificial sweeteners than any other source used. It is also fairly short and direct. It can be determined unbiased and credible due to the fact that it was published through Harvard Medical School. This source would not be optimal for this essay because it provides information showing that artificial sweeteners are unhealthy and lead to weight
America, land of the free and where every vote matters, but does it? The popular vote in america hasn't always mattered and may never matter when the electoral college holds the final poll for presidency. bush was one president who didn't have the popular vote at all, yet he still became president. Does the popular vote matter and should we fix this issue? In reality americans votes don't matter because the electoral college has final say in the presidential election.