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Aspartame Lab Report

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Yeast, Saccharomyces cerevisiae strain D7, is a unicellular, eukaryotic organism. The rapid growth rate of S. cerevisiae allows it to be used to test for mutagens. The mutagenesis of S. cerevisiae can lead to visually observable alterations in its phenotypes when grown on different agar media.

Aspartame is a synthetic compound that is 200 times sweeter than sugar but contains no calories. Hence Aspartame has been used as an artificial low-calorie sweeteners and can be found in over 6,000 food items, and millions of Americans consume aspartame everyday (Lindseth et al., 2014 ). Despite its widespread use, aspartame remains one of the most controversial food additives (Magnuson, 2010).

The role of sweeteners in cancer risk has been widely …show more content…

In their experiments, they used aspartame in concentrations of 1.25ppm, 2.5ppm and 5ppm. After preparing the test-tubes, they incubated the test-tubes at 37 degree Celsius for 3 hours. However, as the experiment was involved human blood, 37 degree Celsius is the ideal temperature. For our experiment, we would keep the treatment of the yeast cells with Redoxon Double Action Vitamin C solution at 30 minutes in view of time constraints. This is also because Saccharomyces cerevisiae yeast strains have the fastest initial growth at near 30 degree Celsius (Walsh and Martin,1977).

Materials:
1. D7 yeast strain (ATCC 201137)
2. Ethylmethane sulfonate (EMS, a mutagen for positive control)(prepared)
3. Sterile water (for negative control)(prepared)
4. Medium plates (3 of each of the following): i. Yeast extract peptone dextrose (YPD) agar plate, ii. Agar plate containing synthetic medium lacking isoleucine, iii. Agar plate containing synthetic medium lacking tryptophan
5. Phosphate-buffered saline (PBS), pH 7.4
6. Water bath at 28°C
7. Distilled water
8. Redoxon© Double Action Vitamin C tablets

Procedures:
1. Resuspend yeast cells in PBS at pH7.4. (around 1 x 105 cells per

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