Audiovisual Exercise On Cdna Cloning And Characterization

 By restriction enzymes then amplified by polymerase chain reaction to make many to millions of copies of a single fragment.

The vital components and techniques of gene cloning are as follows, the DNA sequence that contains the desired gene (EZH2) is amplified by Polymerase chain reaction. PCR was established by Kary Mullis in 1985, popularly known to amplify target sequences of DNA (EZH2) to a billion fold in several hours using thermophilic polymerases (Taq) ,primers and other cofactors (Sambrook and Russell, 2001). Three crucial steps are involved which are Denaturation (at 95°), Annealing of the forward and reverse primers (55-65°) and lastly primer extension (at 72°). After amplification the desired sequence is integrated into the circular vector (pbluescript) forming the recombinant molecule. For the compatibility of the insert and vector, both were digested with (EcoR1) so the same cohesive ends are generated in both, making it easier to ligate. EcoR1 is a restriction enzyme that belongs to the type II endonuclease class which cuts within dsDNA at its recognition site “GAATTC” (Clark 2010; Sambrook and Russell, 2001).

Rate each of the following statements as TRUE or FALSE. Justify your answer with an example or explanation to prove and illustrate your understanding. DO NOT OMIT THIS PART OF THE TEST. True/False answers can be guessed. But when you defend your answer by example or explanation, you demonstrate not only your memory and understanding but also that you can apply what you have learned. The first question is answered for you.

that goes into creating a clone. First, scientist remove a somatic cell from an animal that they

Use the article assigned to you to answer the assigned questions. Upload the answers BEFORE the start of lab on the due date!

nant research, to achieve recombinant DNA. The gene that is the focus of this experiment and is

Another method would be by a process known as Somatic cell nuclear transfer (SCNT), which is sometimes referred to as

Read each question carefully and answer only the question that is asked. Answers for each question should be 2 pages, typed, Times New Roman, 12 point font, double spaced, with one inch margins. This document is already formatted correctly.

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There are no right or wrong answers and it is not a test. It is only a way to help you define your understanding of this

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(B) I would like for the exam to have a little of constructed response, multiple choice, and short stories.

There are three types of ways to produce a clone first one is gene cloning and that creates a copy of the genes or parts of DNA. Reproductive cloning makes a copy of whole animals. Then Therapeutic makes embryonic stem cells. The most used is therapeutic cloning.

The most important improvement in the method of cloning was Wilmut and Campbell's control of the cell cycles of the donor cell nucleus (Chye 2001). They were able to cause the cells to enter G0, a stable stage from which to transfer the somatic cell nuclei, by removing the serum from the cells in the culture (Chye 2001). Since they were able to determine which stage the nuclei were from, the nuclei used to create the fertilized oocytes were in the proper phase of the cell cycle as to promote growth and development (Chye 2001). The embryo was then activated with the use of an electrical pulse, and left for several days to mature (Chye 2001). In the final step, the cultured embryo was implanted into the