B. Cereus Essay

Composers of texts present a biased attitude to the events, personalities or situations represented. In various texts such as Shakespeare’s “Julius Caesar” and Leunig’s cartoon “Yet another picture with the wrong caption”, the composers bias is evident even though conflicting perspectives towards the personality are presented.

An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The first procedure that was done was the gram stain. Under a microscope, if the gram stain is purple, the bacterium is gram positive, if the stain is red, it is gram negative. The next test was the fermentation tests for glucose, sucrose and

In Roman history, some elite men held certain values that they felt strong enough to take their life in order to defend it. In William Shakespeare’s Julius Caesar, there are certain characters portrayed to show how a person’s values or ideas can change their behavior and influence some significant decisions. The protagonist of the play, Marcus Brutus, supports this thought by having an idealistic view on the world and by showing his patriotism toward Rome. In William Shakespeare’s Julius Caesar, Shakespeare uses Brutus as an honorable, idealistic man in order to show the depth that a high-class Roman man will go through in order to defend his honor.

Caesar Augustus the man who found Rome a city of bricks and left it a city of marble. Caesar Augustus, one of the strongest and most strategic leaders in roman history, but this was all much later in his life, he started off in a very poor state yet through hard work and determination he came out on top to be known throughout the generations.

The Unknown Bacteria 36/Bacteria # 2 on a TSA plate was examined by the naked eye and under a dissecting microscope. Bacteria # 2 was approximately 3 - 4 mm in diameter. They were circular in form with an entire margin and a flat elevation. The colonies were rough (granular), translucent, and white brownish color with black granules. The Gram stain resulted in a Gram negative rod. After the Gram stain was completed, the bacteria were streaked on an Eosin -Methylene Blue Agar plate and an Enterotube II was inoculated.

The first result of importance was the result of the Gram stain. The observations of the unknown bacteria from the slant culture after Gram staining showed that the unknown bacteria were Gram negative bacilli (Image 1). After determining the unknown bacteria was Gram negative, an oxidase test was conducted on a sample from the slant culture. The cotton swap with the sample of bacteria did not change color when the oxidase reagent was applied, thus providing a negative result. With a negative oxidase test, further tests were conducted to determine various characteristics of the unknown bacteria. A MR-VP broth was inoculated with a sample from a slant culture of unknown bacteria. After incubation, the methyl red reagent was added to the broth, and the broth turned red, providing a positive result (Image 2). An EMB agar streak plate was inoculated with a sample from a slant culture of the unknown bacteria, and after incubation, growth was found on the plate, providing a positive result (Image 3). A Citrate agar slant was inoculated, and after incubation, growth was found on the media, providing a positive result (Image 4). A Urea agar slant was inoculated, and after incubation, the agar had changed from a peach color to a bright pink color, providing a positive result (Image 5). Using the flowchart (Figure 1) developed from the Table of Expected Results, the lab partners started at the oxidase test. Given the negative result of the oxidase test, the flowchart is

In a laboratory setting, it often becomes necessary to identify an unknown organism. In this experiment, researchers classified an unidentified bacterium based on its physical structure, colony morphology, optimal conditions and metabolic properties. A Gram stain using crystal violet, iodine, and safranin and a simple stain using methylene blue characterized the organism’s cell wall. Cultural behavior was classified by inoculating the organism onto nutrient agar and incubating it at 37° C for 48 hours, and observing its behavior, as well as using SIM medium to test for motility. Optimal growth temperature was

Bacteria are ubiquitous; they can be found on the skin, in the soil, and inside the body. Because of the very nature of this ubiquity, it is important to be able to determine between different strains of bacteria. An example of this is determining the causative agent for a disease so that the patient will be treated with the appropriate antibiotics. It may be important to determine the bacteria in a certain region, because like with enteric bacteria, it is normal to find them in the digestive tract as they are in a symbiotic relationship with our bodies in this area; however, they also cause opportunistic infections in places outside of the digestive tract to our detriment, such as with a urinary tract infection. Some strains of bacteria are common to nosocomial infections, and identifying these bacteria as such helps create the guidelines for healthcare workers in antiseptic technique. All of the morphology and characteristics of each strain of bacteria help us to better understand the role of bacteria in the body as well as helps us understand how they can cause illness, and what treatment regimen to set in place. In lab this semester, a sample of unknown

Preliminary studies help identify Genus species of bacteria. Two different preliminary study pathways must be used since two different pathogens were found in the sample. A dilution and a quadrant streak are the ideal methods to separate pure cultures of bacteria. MacConkey Agar and CAN (MAC) is a selective media that is used for the cultivation of gram negative bacteria. (PEA) is a selective media that is used

Bottone, E.J., Bacillus cereus, a Volatile Human Pathogen. (2010) US National Library of Medicine National Institutes of Health. Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2863360/

In William Shakespeare’s play, Julius Caesar, many themes are developed. One character, Brutus, displays a significant theme that develops through the entire play. The theme that is supported most by this character is the idea that honor and respect are gained through performing works for the good of others instead of for personal gain.

The pre-treatment frequency of bacterial species in the cultures from both groups is detailed in Table III. Streptococcus spp. made up 15 per cent of isolated bac-teria in gr0up A” and 55 per cent in gr0up B (Table III). Haemophilus influenzae was 10 per cent of isolated bacteria in gr0up A and 0 per cent in gr0up B”, and Moraxella catarrhalis was 5 per cent of isolated bac-teria in group A and in group B. Staphylococcus aureus was 0 per cent of isolated bacteria in group A and 15 per cent in group B. S viridans was 10 per cent of isolated bacteria in group A and 0 per cent in group B. S epidermidis was 35 per cent of isolated bac-teria in group A and 10 per cent in group B, while no growth of bacteria made up 30 per cent of group A and 2O per cent of group B”, as shown in Table

These are gram positive bacteria which are found in acidic condition of pH 4 to 4.5 – acidophilic.

2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB), TSI (Triple Sugar Iron agar), Phenol red sucrose, the SIM test, H2S by SIM, IMViC (indole, motility, voges-proskauer, and citrate), Urease (urea broth), PDase (Phenylalanine Deaminase), Lysine Decarboxylase, and Ornithine Decarboxylase. Colonial morphology on EMB was used to

Introduction: Through the conduction of numerous experiments, the identity of two bacterial isolates was determined. The tested specimen was an unknown sample of a mixed culture of two different species of bacteria. The first step that was taken was obtaining a pure culture of each species of bacteria by isolating one species from the other. Once isolation was complete, the isolated cultures were tested using procedures that had been performed during previous lab sessions. A gram stain was performed on the two isolates. The isolate which had tested gram negative was then tested for the presence of cytochrome C and lactose fermentation. For the gram positive isolate, cell shape was determined and a catalase test was performed.