Bacteria Lab

Bacillus, Coccus, and spirillum are the three main kinds of bacteria. Bacillus are rod shaped. Cocci are spherical shaped. Finally spirillum are spiral shaped.

The next step of the project included preparing a Gram stain to discover the cell shape, arrangement, and if the bacteria is gram positive or

Bacteria are single celled organisms. Some classify them as a separate (fourth) kingdom on the tree of life.

Bacteria are prokaryotic, and if you were to observe the two cells under a light microscope, you could distinguish their differences. The biggest distinguishment you can make while carefully observing the two cells, is the lack of a nucleus in a prokaryotic cell as compared to a eukaryotic. Prokaryotes also lack any membrane-bound organelles.

The science of identifying these organisms based on known characteristics is termed bacterial taxonomy. Where each species is assigned to a genus which then follows a level of hierarchy using ranks (family, suborder, order, subclass, class, division/phyla,

4.) To further differentiate the bacteria from other options, obtain 1 TSA plate and perform a

Bacteria are small, unicellular prokaryotic microbes. They have many morphologies, which include rod-shaped, spherical, spirals, helices, stars, cubes, and clubs. Classification of bacteria begins with either aerobic (requiring diatomic oxygen for growth) or anaerobic (not requiring O2 for growth). Bacteria can simply be narrowed down to gram positive (organism that stains purple or blue by Gram stain) or gram negative (organism that stains red or pink by Gram stain). Many physical and nutritional factors influence bacterial growth. Physical factors include temperature (psychrophiles, thermophiles, and mesophiles), pH (neutrophiles, acidophiles, and alkalinophiles), O2 concentration (aerobic

Escherichia Coli, located in one of the main organs known as the large intestine, is a type of bacteria that helps digestion. (Trzepacz, Timmons, and Duobinis-Gray, 2016) For Escherichia Coli to remain stable and alive, it needs specific necessities. These needs are known as the following: Energy, vitamins, and similar compounds. Escherichia Coli is found everywhere!

The purpose of the bacterial unknown independent study experiment completed throughout the course of this lab was to determine the identity of an unknown bacterial species. The unknown bacteria sample was chosen from numerous samples provided by the instructor. The starting unknown sample, unknown #15 was a mixed bacterial culture and a broad approach taken to identify the sample. Various biochemical tests were completed to identify the bacterial species along with the use of databases such as Gideon and Bergey’s to compare the test results of known bacteria to the results of the unknown sample. Information was gathered from the other sources and databases and phenotypic testing completed and the results compared to the database results. Aseptic

During the final experiment for Microbiology will have to figure out the unknown organism given to us. The organisms will be narrowed down by using skills we have learned throughout the lab this quarter and the test results will narrow down to a single organism. The reason to perform experiments that determines the identity of bacterium is to find out how to treat it or what infections it is causing. In a controlled environment, students learned how to use various methods to determine how to differentiate between multiple bacteria. This lab report will be detailed steps explaining how to determine the identity of my unknown organism number 12.

The students will be able to analyze the size, margins, elevation, and color of their bacteria.

My name is strep. Streptococcus Mutans to be exact. I live in a diverse community but my family make up about twenty percent of the world we live in. Our home is called mouth. We have lived here for as long as I can remember. From what I have been told we lived here before there were even homes for us. As a baby our host was kissed by a caregiver who transferred my family to our host. I wasn’t around yet but I hear we had to live down in the grooves of the tongue at that time. Then when teeth began erupting we were able to upgrade into better homes but some of my family still lives down in those valleys. We’re a bacterium that is normal to have so our host has never really tried to kick us out but we’re not the cleanest of microbes and have been known to cause some tooth decay here and there. I am writing this journal to keep record of the things that have been happening to us lately.

In less than 2 months, Massachusetts General Hospital in Boston will start an experiment testing the effects of gut bacteria on human weight. To get the gut bacteria into the body, the participants will consume “poop pills” that are similar to capsules of fecal matter provided by OpenBiome, a nonprofit stool bank. One of the large concerns with using these “poop pills” is the safety of eating stool, but the producers of these “poop pills” go through extensive screening before accepting a stool donation. The researchers are optimistic as to the effects of these “poop pills” and hope that they could help treat obesity along with a healthy diet and exercise. In this experiment, the independent variable is the thin person’s fecal matter and the dependent variable is the amount of weight lost. The control group is the group of participants that ingest a placebo while the experimental group is the group that receives the thin person’s fecal matter.

The original plates were swabbed with defective bacteria that did not grow to show sufficient, so new plates were made in replacement and the following results and conclusions are based off of observations from the new plates. The hypothesis stated earlier was rejected by this experiment because in the hypothesis rubbing alcohol was predicted to have the largest zone of inhibition, but according to the class and overall average, Betadine has the largest zone of inhibition diameter at 42.2 mm for the class average and 39.6 mm for the overall average. As shown in the graph and table, the largest diameter lengths in order from least to greatest was Betadine with a class average of 42.2 mm and an overall average of 39.6

Bacteria vary greatly in terms of their characteristics and morphology. Colonies can be classified according to their colour, form, elevation, margin and size. Pure cultures of microorganisms