Bacteria Growth Lab Report

Steeve & Mallison (1975) stated hand hygiene has often been singled out as the most important procedure in preventing infection. Guidelines from national and international infection prevention and control organizations acknowledged that hand washing is the single most important procedure for preventing infections (Ganner and Favero,1985).

Dirty hands is the common source of spreading infection. It is very important to keep hands clean to avoid getting infected and spreading infection in the community. It is important to wash hands to keep hands clean. There are two ways to keep hand clean, one way is wash hands with soap and warm water while rubbing hands together for minimum 15 to 30 seconds. Indication of washing hands with soap and water is when hands are visibly dirty, before and after eating, feeding, using the toilet, after coughing or sneezing, after using gloves, taking care of patients. There is also second way to clean hands, but it is advisable to wash hands with soap and water all the time, but it can ignore when soap and water is not available so it is okay to use hand gel or foam in the form of sanitizer. This helps to clean hands or kill germs when hands are not visibly dirty.

Bacteria can exist almost everywhere, some are harmless, and some are harmful. There are thousands of different types of bacteria and they fall

Washing your hands before and after doing individual jobs, using the correct PPE for the task you are carrying out, Gloves, Aprons etc. This can stop the spread of fluids and germs which can be carried on clothes. Simply covering your mouth when coughing and sneezing then washing your hands will stop the spread of possible infection. Many surfaces can hold germs and bacteria, so even after touching any surfaces hands should be washed or gloves worn where ever possible.

Bacteria is a single celled organism, bacteria have evolved to thrive in almost any environment and can be found in almost any substance/surface and also in the human body, only 1% of bacteria is actually harmful.

This experiment was conducted to find the genus and species of an unknown bacteria prescribed by the lab teacher, which was unknown bacteria GA3 in my case. Identification of unknown bacteria techniques are used on an every day basis to figure out what type of bacteria it is and to find the best method of how to treat a patient with this bacteria (1). All five “I’s” of Microbiology were used in the testing for the unknown culture. Inoculation was used several times to put the unknown culture into agar plates or into biochemical test tubes. After Inoculation of these tubes or plates, they always were placed into the incubator for further growth and development. Isolation was used to make sure we got the correct bacteria we were testing for. After each further isolation, we gram stained the culture and inspected the culture under a microscope to further help in the identification process of the unknown bacteria. Multiple tests were done on the unknown culture to make sure we were confident in what kind of bacteria the unknown was.

Citrobacter Freundii is a species of bacteria that can be potentially harmful to humans. It is known to cause meningitis by protruding into the brain and replicating itself (1). The Citrobacter species has also been found as a cause of some urinary tract infections, diarrhea, and even gastrointestinal diseases and symptoms (3). C. Freundii can be located in a wide variety of soils and water (3). Lastly, it is also the cause of many nosocomial infections due to its presence in water (1).

To help the prevention of infection spreading is by knowing the method of washing your hand because we carry most bacteria sue to the open air that we come in contact with. For example we use are hands to shake hands with someone holding or touching objects. When you are performing any form of hand hygiene you will need to make sure that you have washed your hand with anti-bacterial liquid soap; this is to help prevent any bacteria which we already have on our hands. In all health and social care settings an automatic liquid dispenser should be placed so that when washing hands any individual doesn’t touch or need to even sneeze any part of the liquid

In the world of microbiology it is vitally important to be able to discern the identities of microorganisms. Not only is it important in a lab setting but as well as in healthcare in general. Properly identify what strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium. Using all the knowledge of procedures and lab techniques identify the unknown and discuss all the tests you performed.

This experiment is about bacterial growth. We will demonstrate a bacterial growth curve using a closed system. Bacterial growth usually takes up to 12-24 hours to get an accurate result so we will be monitoring this growth between two classes. We also used different methods to determine bacterial growth as well as a few different calculations. One way of receiving data is by using a spectrophotometer where we will record the absorption at a given time to create the bacterial growth curve. We also used the plate count method after performing a serial dilution to calculate the actual cell density at different times given. By using this method we can count the population number of the same given and see the maximum cell density

Being able to control bacterial growth is something that is important in our everyday lives. As shown in the previous labs, bacteria can grow and create colonies extremely quickly especially in the right environments. By acknowledging this, it is then important to get an understanding of how bacterial growth can be controlled by humans. To control microorganisms it means to inhibit their growth (static) and or kill them (cidal) (Kenneth Todar, 2015); therefore since focusing on bacteria the terms bactericidal and bacteriostatic are both extremely important for this lab. One broad method we will use to control bacterial growth is heat. The amount of heat needed to control bacterial growth is different for different species of bacteria (Kenneth Todar, 2015). Bacteria can also respond differently depending if moist heating method such as an autoclave with steam is used, or a dry heating method such as inoculating a loop over a fire is used (Kenneth Todar, 2015). UV works by damaging the cells DNA, without proper DNA, the cells will die and the object

I would test more brands of mouthwash. I would also recommend timing and checking the plates after so much time and she if any changes occur. I also would test this experiment in my mouth when bacteria is present from not brushes the teeth. Then applying mouthwash for so long and then swabbing and testing the cleanliness of my mouth afterwards. I would also do this will my mouth after eating some sticky candy and testing my mouth after adding mouthwash . I would swab and test my mouth after mouthwash was applied for a period of time. Then check my results. This experiment could be expended in a few ways to test the effectiveness so I was going to do this again I would possible try that example and see what my results also could

Nutrient Broth and Nutrient Agar were used to inoculate bacteria taken from different surfaces. Nutrient agar plate was inoculated with a sample taken from skin surface. A sterile cotton swab was first immersed on sterile water, then, rubbed against the skin with swirling motion and transferred to an agar plate by rubbing

Each mixed culture that was tested had one gram positive and one gram negative bacterial species. The possible species of bacteria that could have been isolated from the mixtures included the following: Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Enterobacter aerogenes, Salmonella enterica, and Pseudomonas aeruginosa. The identities of the unknown species were determined through comparing the experimental data against data acquired from earlier experimentation.

A defensive strategy is important when trying to avoid infecting oneself with an illness lying in wait (Wisegeek.com, 2011). According to Mayo Clinic (2009), frequent handwashing is one of the best ways to avoid getting sick and spreading illness. Although it is impossible to keep hands germ-free, washing hands frequently can help limit the transfer of bacteria, viruses and other microbes.