Bacteria and Degrees Celsius Essay

A mixed culture of two unknown bacteria was provided by the instructor. The methods used for

An unknown bacterium was handed out by the lab instructor. The methods that have been learned so far in identifying bacteria were applied to this unknown. Procedures were followed as stated in the lab manual and biochemical test handouts. The first procedure that was done was a gram stain followed by a streak of the unknown on a TSA plate in order to determine the gram reaction and observe the colony morphology. After that, specific biochemical tests were performed for gram positive, since unknown number five was determined to be gram positive rod. The other tests were performed in this order: Mannitol Salt (MSA) streak, Blood Agar streak, Catalase test, Nitrate Reduction test, and Phenyl

An unknown bacterium 15 was awarded and labeled at the table ready to be identified. Using the skills and test that are taught and learned in microbiology were applied into learning what the unknown bacteria culture was. There were multiple procedures and test done in order to gain all the information needed to determine which bacteria was given. In order to find what the bacteria was the first step was finding the right environment and temperature that would allow the bacteria to thrive and grow. Determining this is one of the most important steps in being able to obtain conclusive results that would allow the results of the test to be accurate and correct. Without the correct temperature and environment the bacteria will give inconclusive results which will alter and skew the end results and may lead to the wrong conclusion. By using the methods that were obtained and learned through the microbiology class allowed the skills and knowledge to determine the bacteria and execute the tests in order to determine the culture.

The sole purpose of this project was to identify an unknown bacteria sample #7. Many tests were carried out to determine what this unknown was. Aside from a microbiology lab, understanding and identifying various organisms are important in disease processes, pharmaceutical arenas, and even in the industrial field. Proper lab techniques, including aseptic technique were used throughout the process of identification.

Epidemiology – the science that studies when and where diseases occur and how they are transmitted. CDC, Atlanta, tracks and traces diseases. ONE World (the idea that it’s all in one place, it could happen anywhere; locally, states, large counties track the incidences and occurrences of disease)

After gaining some knowledge about bacteria, we were giving an investigating bacteria growth lab to do. Our objective was to observe the conditions required for bacteria to grow and to test the effectiveness of substances that may be antibacterial, disinfecting, and or sanitizing. My group and I began our procedure by gathering all the bacteria by swabbing our necks and mouths. After this, we inoculated the culture by rubbing the bacteria on the agar, a nutrient rich gel made from sea kelp, on the bottom side of the container where we grow bacteria, the Petridish. We hoped for the results to come back with little or even no colonies and an immense zone of inhibition around the tiny circle cut out of filter paper covered in toothpaste, Neosporin, and Chlorhexidine Gluconate 4% Solution.

Sidney LeVine Ms. Kim Science 6 Jan. 14 2015 Reverse Osmosis filtration method Paragraph 1: Overview Reverse Osmosis filtration method, and explain what “reverse” and “osmosis” means, explain theseus, explain Paragraph 2: Explain why it is necessary for this filtration method to be used (disease/bacteria removed), and how if affects the people using it and the people applying it to their everyday lives. Paragraph 3: Pros and Cons overviewed with explanation Paragraph 4: Review Reverse Osmosis, briefly cover highlights from text, finished. Theseus statement: Reverse osmosis is an effective filtration method that positively affects the surrounding community by giving them guaranteed clean tap water. Paragraph 1: Reverse osmosis is an effective filtration method that positively affects the surrounding community by giving them guaranteed clean water.

Asepsis can be defined as the complete absence of bacteria, fungi, viruses or other microorganisms that could cause disease. Aseptic techniques refer to a set of skills that are used to ensure any environment being examined or dressed remains free from micro-organisms by not directly touching the wound or any other surface that might come into contact with the wound (McFerran and Martin 2008).

An organism of unknown origin was selected by the course instructor in an effort to properly identify the organism with the utilization of a series of biochemical tests. This exercise was of extreme importance during the termination of the course because it was essential that each student be able to apply the practical knowledge learned through class lectures, and lab practicals in an effort to individualize such experience so that each student could properly handle a bacterial culture and identify its origin. In essence, the task of identifying the selected organism via various test is essential in order to determine key features of such organism. The test results ultimately disclose how the organism functions, and this information is useful for proper identification and handling of such culture.

2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB), TSI (Triple Sugar Iron agar), Phenol red sucrose, the SIM test, H2S by SIM, IMViC (indole, motility, voges-proskauer, and citrate), Urease (urea broth), PDase (Phenylalanine Deaminase), Lysine Decarboxylase, and Ornithine Decarboxylase. Colonial morphology on EMB was used to

Each mixed culture that was tested had one gram positive and one gram negative bacterial species. The possible species of bacteria that could have been isolated from the mixtures included the following: Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Enterobacter aerogenes, Salmonella enterica, and Pseudomonas aeruginosa. The identities of the unknown species were determined through comparing the experimental data against data acquired from earlier experimentation.

The other type of process for bacterial growth is anaerobic respiration, these bacteria grow only if there is oxygen present. The final requirements for growth are ph. level and time. Each type of bacteria flourish in certain levels of ph., for example acidophiles grow best with a ph. level under 5.5. Then if the requirements for the bacteria are met more efficiently then the bacteria grows at a more rapid pace.

Broth cultures of E.coli, Micrococcus luteus and Vibrio natriegens were streaked onto the respective sections. The plate was then incubated at 37oC for 48 hours. The process was then repeated on nutrient agar plates with 5, 6.5 and 10% salt.

It is one of the primary diagnostic method of microbiology and used as a tool to determine by letting the agent multiply in a

A number of factors must be considered to enable the use and control of the MICP process in field applications, including the concentrations of bacteria solution, the concentrations of the chemical solutions, in addition to methods to introduce the bacteria and these chemical solutions to the soil.