Bacteria and Degrees Celsius Essay

765 WordsOct 21, 20104 Pages
Aseptic Technique & Culturing Microbes Purpose: To learn and employ aseptic technique and basic forms of culture media as well as become familiar with the basic requirements of microbial growth and the methods used to control microbial growth. Procedure: Obtained a small Styrofoam cooler placed two small light bulbs in side and observed temperature over 24 hours to ensure temperature could be maintained between 98-100 degrees. Using a 10% bleach solution I then cleaned my work area. Transferring one capsule of L. acidopholis into a tube of MRS broth using the aseptic transfer technique then marked a line on test tube to record sediment. Labeled tube of nutrient broth S. epidermidis, then using a sterile swab obtained sample of…show more content…
Bacterial refers to killing bacteria and bacteriostatic refers to inhibiting the growth of bacterial cells. Sterilization is the complete destruction or elimination of all viable organisms. There is no degree of sterilization either it is or is not. Disinfecting is lowering the number to safe levels B. List five sterilization methods, how they work, and what they are used for. 1. Filtration – 2. Boiling – 3. Autoclaving – 4. Dry Heat – 5. Incineration - What is pure culture? Why is it important to work with a pure culture? A pure culture is one in which all organisms are descendants of the same organism, it is important to work with a pure culture due to microorganisms usually exist as mixed populations so to be able study, characterize, and identify microorganisms they must be in the form of a pure culture. What is aseptic technique? Why is it so critical? Aseptic technique is the method of preventing unwanted microorganisms from gaining access, this is crucial for allowing the transfer of growing organisms to a sterile medium with out introducing any outside contaminants. C. Describe three common forms of growth that you are likely to see in a broth culture? Pellicle – mass of organisms floating in or on top if broth Turbidity – organisms appear as a general cloudiness through the broth Sediment – mass of organisms appears as a deposit at the
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