Bacterial Conjugation Experiment Essay

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Bacterial Conjugation Experiment

INTRODUCTION: Bacteria, in general, reproduce asexually, but in order to increase diversity, they have developed a mechanism for transfer of genetic material from one bacterium to another. The ability to perform this transfer is conferred by a set of genes which are called F for 'fertility.' These genes exist on a small, circular piece of deoxyribonucleic acid (DNA) that replicates independently from the bacterial chromosome, or they can be integrated into the chromosome. The bacterium containing this gene (sometimes referred to as 'male' or F+) extends its pilus to a neighboring bacterium (sometimes referred to as 'female' or F-), and the two cells are attached. This process is called conjugation.
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To make the complete media, appropriate amounts of each amino acid, glucose, vitamin B1, and streptomycin were aseptically added to the MM56 media and poured into sterile petri plates. The selective media was prepared so that 84 plates were made. These plates were made much in the same way as the complete plates, except that 28 of the plates contained all of the reagents except proline, another 28 were without histadine, and another 28 were without threonine. These were labeled with "pro-", "his-", and "thr-".
Viable Counts: The strain used was Escherichia coli K12. The donor and recipient cultures were kept as much as possible in a water bath at 37ºC. Viable counts of the donor (Hfr) and recipient were done on complete MM56 and Luria agar. Serial dilutions were performed to obtain a 10-7 and 10-8 dilutions of the recipient, and 10-2, 10-7, and 10-8 dilutions of the donor. Two Luria plates were inoculated with 1 ml each of the 10-7 dilution of the recipient. Two Luria plates were inoculated with 1 ml each of the 10-8 dilution of the recipient. Two Luria plates were inoculated with 1 ml each of the 10-7 dilution of the donor. Two Luria plates were inoculated with 1 ml each of the 10-8 dilution of the donor. Two complete MM56 plates were inoculated with 1 ml each of the 10-7 dilution of the recipient. Two complete MM56 plates were inoculated with 1 ml each of the 10-8 dilution of the recipient. One complete MM56 plate was inoculated with 1 ml of the 10-2 dilution of the
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