Bacterial Growth Lab : Bacteria

When reflecting back to experiment 3, Aseptic Technique and Culturing Microbes, I realized the large amount of microorganisms that can be found in everyday life. Many different types are found with in the human body. Theses experiments focused on two types of bacteria. First was Staphylococcus epidermidis, found on the skin, and second was Lactobacillus acidophilus, found in the gastrointestinal tract. Both have similar needs for growth when it comes to temperature, however, different growth environments are used.

Conclusion: The results from the bacterial transformation lab did support my hypothesis. My hypothesis said that the petri dish labeled +LB/AMP/ARA would have green glow bacteria. This was proven true as this particular petri dish had 34 bacterial colonies that glowed neon green when shown under UV light. One primary error I made was that I did not spread the bacteria as much as I needed to. This resulted in the bacteria being centered in the middle. In the future, I would take more time to ensure that the bacteria was completely spread out. Another error I made was that I applied the DNA before adding the bacteria. To prevent this mistake, I would take more time to do the

Bacteria are the most common and ancient microorganisms on earth. Most bacteria are microscopic, measuring 1 micron in length. However, colonies of bacteria grown in a laboratory petri dish can be seen with the unaided eye. When considering the pH level, bacteria are classified as either acidophiles (acid-loving), neutrophiles (neutral ph range), or alkaliphiles (alkali-loving). The one that causes disease in humans would be the neutrophiles, which have an ideal pH range of 5.4 to 8.0. There are exceptions, however, like Alcaligenes faecalis and Vibrio choleae, which are both alkaliphiles and can infect humans. There are physical and nutritional factors that affect bacterial growth in the environment. Sterilization is needed to keep an environment free from bacterial growth. Failure to sterilize bacterial growth in our food products today leads to the unfortunate consequence of food poisoning.

E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source, nutrients, pH, and temperature’. Therefore, MacConkey Agar being the medium for its growth will enable us to achieve the experiment

Culturing bacteria on a media has been an important laboratory technique since Robert Koch discovered how to grow bacteria in a petri dish. Bacteria will cultivate successfully on the media if the right nutrients, temperature, humidity, pH and any other specific conditions were provided. The most basic and common medium of growth on the nutrient broth, which contains water, beef extract and peptone. Although most bacteria will grow in this medium, some bacteria are fastidious and require special growth supplements such as blood or sugar. Further in mixed populations where it would be difficult to isolate the minority type of organism in a simple medium. Therefore, media can be divided into different types depending on the chemical composition in order to help isolate selected microorganism’s. The commonly used media are differential, enriched and selective media.

Introduction The main purpose for completing the experiment is to understand bacterial growth. In order for bacteria to grow effectively, two important factors are required, physical and nutritional. Physical factors include temperature, pH, osmotic pressure and gaseous requirements1. Bacterial growth is temperature sensitive.

After conducting the necessary experiments, it was found that the two bacteria in the mixed culture of TSB test tube labeled 10, were Proteus mirabilis and Staphylococcus saprophyticus. Proteus mirabilis being the rod shaped, gram-negative bacteria that grew were the small, shiny, and round with a convex elevation, and Staphylococcus saprophyticus being the cocci shaped, gram-positive bacteria that grew a creamy, off white color and round. Proteus mirabilis comes from the Enterobacteriaceae family, which consist of gram-negative bacteria some being harmless, while the majority ae considered as pathogens. “Within this family are genera such as, Shigella, Salmonella, Klebsiella, Serratia, and Enterobacter (Guentzel, 1996).” Staphylococcus saprophyticus

This research brings us one step closer to fully understanding the bacteria that reside in our

Bacteria can be found everywhere and in everything. It’s in the food you eat, on the things you touch, and even in the air you breathe.

Objective: In the past couple of weeks, our class received a single broth that contained two species of bacterium. One bacterium was gram positive and one bacterium was gram negative. We were required to use our techniques and knowledge we learned in class to separate and identify the two species we were given in our broths.

After the completion of this lab, I discovered that even harmless bacteria does indeed smell horrible and since our colonies were harder to see closed, we had to open them and experienced the odor firsthand. I also learned that this process could be/is beneficial to health care to create proteins like insulin and help with diabetes treatment. And learned how to use a pre-set micropipette to transfer small amounts of substance, even if it was under Mrs. Jewell’s

It turns out microbes don't wait for five seconds, or even 1 second to hop on to your food. Even brief contact with a contaminated floor will contaminate food wet or dry! Bacteria will adhere to food almost immediately, but time does matter. The food left on the floor for five seconds acquired a lot of bacteria, but the food left on the floor for 30 seconds picked up a few colonies more. Imagine if you left it for a full minute, or even 10 minutes... the amount of bacteria would be ten times greater! Furthermore, don't think your food is safe because the floor "looks clean" or the food that you dropped on it does. Floors make great homes for bacteria. Also, floors come in contact with our shoes on a regular basis, and the University of Arizona

The ability to cultivate and identify organisms of interest in the laboratory without contamination is essential for the study and classifications of many life forms. Different techniques were used to differentiate the organisms. This process of transferring a microbe from one medium to the next is called inoculating. The organism chosen in class will be used to construct further testing. The purpose of this lab experiment was to find the identity of the unknown bacterial culture and use all the methods and the assessments that were introduced in the class. By doing that we had to use different techniques that guide us to find the right bacterium. A flowchart was given for the unknown to help us find the right bacterium that

It is fascinating to look around and see how much diversity exists on earth. Perhaps even more interesting is the diversity that exists far beyond the naked eye. Over the past several decades, it has been determined that microorganisms account for the majority of the biomass on earth and new discoveries about these organisms are constantly being made (4). Bacteria make up a very large domain of the prokaryotes, or single-celled organisms. Bacteria have an enormous range of properties. Some can be infectious and lead to maladies and fatalities; others can benefit us by contributing to medications, foods, and even maintaining natural body functions like those in the digestive tract. Bacteria are virtually everywhere. It is for these reasons that it is vital we learn as much as possible about the once unseen organisms. Studying their physical and biochemical features can help us understand how to

Okay class have a seat please, today we are going to be going over something fascinating. Today we will be going over bacteria! If I Must remind you guy’s, a man from the government will be coming in to see what I Have discovered so please show him respect and show me attention. (Knocking on the door) Oh! Gracie would you get that please. Class I Would like to introduce you to our guest, Mr. Miller. Okay shall we get started! First I Would like to go over what I Have discovered is the most remarkable thing anyone has possibly came across and i'm honored to be presenting this to you. What I Have found is something called bacteria, not the good kind but the kind used in wars and deadly attacks. Sadly we can't see it with the human eye but thanks to technology we have microscopes, so please line up and one at a time look through the tube and observe the bacteria.