Bacteriology Lab

70µL of competent E.coli are added to both test tubes; pUC18 and Lux (Alberte et al., 2012). Both test tubes are then tapped and placed back into the ice bath for 15 minutes. While waiting, another test tube is obtained, filled with 35µL of competent cells and labeled NP for no plasmid. A water bath is preheated to 37 degrees Celsius and all three labeled test tubes are inserted into the bath for five minutes (Alberte et al., 2012). Using a sterile pipet 300µL of nutrient broth are inserted into both the control and Lux test tubes and 150µL are inserted to the no plasmid test tube to increase bacterial growth. All three test tubes are then incubated at 37 degrees for 45 minutes. Six agar plates are obtained and labeled to correspond each test tube, three of the plates contain ampicillin. A pipet is used to remove 130µl from each test tube containing a plasmid and insert it into the corresponding agar plate. For this, a cell spreader is first

There were two tubes used in this process: the tube that contained the primary culture and the tube that contained the nutrient agar where the unknown bacteria would grow. First, the inoculating loop was flamed. After removing the caps of both the test tubes, they were flamed to prevent contamination of the unknown bacteria. The inoculating loop was cooled for a few seconds and was then placed into the test tube containing the bacteria. The inoculating loop with the bacteria was placed into the nutrient agar test tube for cultivation. Before the test tubes were capped, they were flamed once again. Also, isolation of the unknown bacteria had to completed. Nutrient agar was placed in the petri dish, and was left to gel for a few minutes. After the agar gelled, the inoculating loop was used to acquire bacteria and streak the unknown onto the plate for

After the trials were completed, a 1:9 bleach to water solution was poured on top of the prepared agar plates. This safety precaution was used to kill the bacteria in preparation of disposal of the agar plates. After the bleach solution was applied to the agar plates, the solution stayed on the agar plates for approximately 5 minutes. The bacteria on the agar plates turned a yellow color after the five minutes and were disposed of. Each safety precaution implemented in this experiment was to ensure the safety of the experimenter, and other

One with nutrient agar but NO E. coli All of the petri dishes received the same volume of agar, and were the same shape and size. During the experiment, the temperature at which the petri dishes were stored, and at the air quality remained the same. After one week the amount of bacterial growth was measured. Independent Variable: Type of agar (nutrient agar, mannitol-salt agar, MacConkey agar, LB agar) Dependent Variable: Bacterial growth

In this experiment, pH indicators, colour indicators, metallic ions, and Kovac’s reagent all aide in the differentiation of different bacteria under different conditions. Proteus vulgaris for example is a rapid production of urease and this is shown through the phenol red indicator, turning from yellow to pink. This experiment is usually done to distinguish from Proteus and other bacteria, which helps to isolate these bacteria since they are infectious (O'hara, et al., 2000).

On August 19th, 2015 this experiment was performed, by 6 separate lab groups.The experiment began by measuring 1 Ml of E. coli into a pipette and pump, then placing the bacteria into a culture medium. The E. coli and medium were then swirled together for a period of 15 minutes, until completely mixed. This mixture was then poured into a petri dish and allowed to solidify for 45 minutes. After the 45 minute solidification time, 5 small paper disks were inserted into the dish. 4 of the disk contained treatments of antibiotic and 1 was left untreated. The

The first day an unknown sample was assigned to each group of students. The first test applied was a gram stain to test for gram positive or gram-negative bacteria. The morphology of the two types of bacteria was viewed under the microscope and recorded. Then the sample was put on agar plates using the quadrant streak method for isolation. There were three agar plates; one was incubated at room temperature, the second at 30 degrees Celsius, and the third at 37 degrees Celsius. By placing each plate at a different temperature optimal growth temperature can be predicted for both species of bacteria.

In our Sterilization Lab, Alex and I swabbed the back of someone's hand and the inside of a toilet for testing bacterial growth. We had 3 petri dishes. In the first one we used what we swabbed off of someone’s hand. In the second, we used what we swabbed from inside a toilet bowl. And in the third we mixed them both.

Introduction: A frequent matter in the science, medical and pharmaceutical world is identifying unknown bacteria. Throughout the past months of this class we have learned lab technique and how to do a variety of different tests on bacteria. Microbiology is not only an academic understanding of microorganisms but learning how to practically use lab procedures to properly identify and test organisms. There are several reasons one might need to identify a bacteria. It could be to find out the causative agent in a patients disease or to figure out the antibiotics that need to be administered.

The experiment was to swab bacteria onto the petri dish and see how the bacteria grew. The purpose of the experiment was to learn how bacteria grows and what kinds of bacteria there were on every day surfaces. State Problem/Purpose The objective of this lab was to observe the growth of bacteria.

Title Page  Introduction The purpose of this lab is to use several techniques to determine the identity of an unknown bacterium. Many of these tests involve the differences between Gram-positive and Gram-negative bacteria. Gram-positive bacteria have a thick peptidoglycan wall while Gram-negative bacteria have a much thinner peptidoglycan wall. This leads to a number of differences in what media each kind of bacteria can grow on and their physical properties. Techniques used in this experiment include growth tests on different media, microscopy of a wet mount, PCR amplification and purification, gel electrophoresis, the catalase test, MSA plates, the KOH string test, and cycle sequencing.

Being able to control bacterial growth is something that is important in our everyday lives. As shown in the previous labs, bacteria can grow and create colonies extremely quickly especially in the right environments. By acknowledging this, it is then important to get an understanding of how bacterial growth can be controlled by humans. To control microorganisms it means to inhibit their growth (static) and or kill them (cidal) (Kenneth Todar, 2015); therefore since focusing on bacteria the terms bactericidal and bacteriostatic are both extremely important for this lab. One broad method we will use to control bacterial growth is heat. The amount of heat needed to control bacterial growth is different for different species of bacteria (Kenneth Todar, 2015). Bacteria can also respond differently depending if moist heating method such as an autoclave with steam is used, or a dry heating method such as inoculating a loop over a fire is used (Kenneth Todar, 2015). UV works by damaging the cells DNA, without proper DNA, the cells will die and the object

Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. These tests may help in determining a particular pathogen’s growth needs.

This experiment is about bacterial growth. We will demonstrate a bacterial growth curve using a closed system. Bacterial growth usually takes up to 12-24 hours to get an accurate result so we will be monitoring this growth between two classes. We also used different methods to determine bacterial growth as well as a few different calculations. One way of receiving data is by using a spectrophotometer where we will record the absorption at a given time to create the bacterial growth curve. We also used the plate count method after performing a serial dilution to calculate the actual cell density at different times given. By using this method we can count the population number of the same given and see the maximum cell density

Another purpose of this experiment is to stress the importance of knowing the identity of a microorganism. Knowing the species of microorganism present in a sample provides a