Essay about Basic Chemistry for Investigating Living Systems

7868 WordsFeb 20, 201332 Pages
LABORATORY 3: LABORATORY 3: Basic Chemistry for Investigating Living Systems Abstract The purpose of this experiment was to explain how colorimetry can be used to qualitatively detect cellular chemical components; to chemically differentiate between proteins, sugars, starches, and lipids; to identify the roles of molecular components in living systems; to comprehend the value of using a systematic approach to research; and to describe why hypotheses, controls, standards, and quality control are important in scientific research (Vorndam, 2002, p. 92). Basic Chemistry for Investigating Living Systems Abstract The purpose of this experiment was to explain how colorimetry can be used to qualitatively detect cellular chemical…show more content…
Clean the graduated cylinder and graduated pipet with distilled water for use in the next dilution. (Vorndam, 2002, p. 97) Calculation: Egg white contains ~10% albumen protein, so 2 mL egg white x 10% = 0.2 mL albumen. Thus, 0.2 mL ÷ 20 mL solution x 100 = 1% albumen solution. (Vorndam, 2002, p. 97) Alpha-amylase Solution Preparation used in Exercises 1 and 2: Find the dropper bottle marked “alpha-amylase.” Carefully remove the bottle cap from the dropper bottle and then remove the dropping tip. This can be done by first placing a small clean piece of aluminum foil or plastic wrap over the tip. Then use your fingers or pliers to gently wiggle the tip upward to free it from the bottle. The foil or plastic will help to prevent contaminating the tip with foreign substances. Place the removed tip on some foil or plastic until needed. Note the instructions on the dropper bottle, and use the amount of water specified there for the following procedure. (Vorndam, 2002, p. 97) Place an aliquot of distilled water into a clean beaker. Use the beaker to pour the exact mL of distilled water into the graduated cylinder. It helps if you use a long-stemmed pipet to control the water when the solution is close to the mL mark. Pour this aliquot of distilled water from the graduated cylinder into the dropper bottle containing pre-measured alpha amylase powder. Carefully replace the dropping tip, still covered with foil or plastic. Remove the
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