Bathroom Door Research Paper

Lab Day 2: The first procedure that was done was a simple stain to identify the bacterial shape. Bacteria tends to be transparent, so a stain must be use to color the cells of the bacteria so it can be viewed under a compound microscope. After heat-fixing three separate loopfuls of my unknown bacteria onto a slide, I used Methylene blue, Safranin, and Crystal violet to stain the three different samples. After rinsing the slide and observing my finding under the microscope, I

Crystal violet stains the cell of the bacteria and is placed over the heat fixed slide for one minute then rinsed with H2O. The mordant that binds the stain is gram’s iodine and is recommended to be present on the slide for one minute. Alcohol is used as the decolorizer and is dripped over the recently rinsed slide until the slide runs slightly blue. If the bacterium retains the crystal violet it is due to the thicker peptidoglycan in the cell wall (gram-positive). The bacteria will then be stained with the counterstain safranin for 45 seconds. When crystal violet is not retained in the cell wall of the bacterium it will stain pink (gram-negative). A gram stain was immediately performed with the recently inoculated streak plate. An inoculating loop was used to gather some of the bacteria from an isolated colony. The loop was placed on a new slide and the transferred bacterium to the slide. The slide was dried, heat-fixed, and the gram stain process just described was completed. After microscopic analysis, it was determined the first unknown bacteria was a gram-positive cocci. The second unknown was determined to be gram-negative cocci. With this information a set group of test decided and performed based on the Unknown Bacteria Flow Chart as provided in the lab manual (Pg.) these test and the results are shown in record on the following pages. These are separated for the gram positive and the gram negative

For the first day, I received my unknown bacteria number 110 and I stared to observe that tube. The tube has normal color, creamy, and little bit cloudy. First, I made two new slants to get the strong and alive bacteria for further text. One tube was incubated at 250C and other at 370C. Next, I started to do my Gram Stain. After I finished the smear, heat fix, and cover the slide with all chemical, I saw the pink color stick onto the slide. I knew that this bacterium is gram negative. Then, I looked up the slide though the microscope form 10X -> 40X ->oil ->100X. I saw the bacteria which have rod shape with chains and pairs. I want make sure one more time that bacteria are gram negative or positive, so I stared to set up EMB and PEA plates to get the result for the next class.

The Swinging Doors, in Spokane, is a lively sports bar serving traditional American fare and plenty of cold drinks. Appetizers, like the sriracha glazed fried shrimp, the beer battered mushrooms and the garlic parmesan fries, are packed with flavor and fun to share. Popular entrees include the Swinging Doors Broasted Chicken, served crispy and juicy, the mac and cheese, loaded with sharp white cheddar and the deep fried Alaskan cod with tasty dipping sauce. Diners rave about The Swinging Doors' burgers and sandwiches, such as the house specialty Doors burger with double beef patties, double cheddar cheese and double bacon, for a real treat. Foot long, decked out hotdogs are other popular menu items, as are the all day breakfast choices.

The purpose of the Gram Stain test was to identify the morphology and gram nature of the unknown bacteria (Delfiner, Martinez, & Pavia, 2016). First, a mixed smear from the unknown bacterium was placed on a microscope slide. Once the mixed smear had air dried it was then heat fixed to adhere the bacterial cells to the slide. After the heat fixation, a series of stains/solutions were added to complete the Gram stain test. The series of stains/solutions included Crystal violet (1min), Iodine (1min), ethanol (2-3 drops), and Safranin (30 sec). Also, after the addition of the Crystal violet and Safranin, DI water was used to remove the stains. Lastly, bibulous paper was used to blot dry the microscope slide and the slide was placed under the microscope. To clearly identify the morphology and gram nature of the unknown, a drop of oil immersion was placed on the slide to utilize the oil immersion

One of the biggest challenge of having glass shower doors is keeping them clean. Here are four household products you can use to keep your glass shower doors clean.

On my first day of the project, I received my reserve stock and was so excited to get started. Once I got that, I prepared my Gram stains. As a refresher, a Gram stain is a stain that reveals if the bacteria is Gram negative (pink) or Gram positive (purple). I ended up needing to do 3 Gram stains because I forgot to heat fix the second one and got shotty results. The first stain was beautifully displayed

From one of my modified streak plates I was able to successfully isolate my Gram positive bacteria. By my 6th plate, there was a distinction between my Gram positive and Gram negative in morphology. To make sure it was absolutely pure I continued to streak it two more times. Once I had a pure plate, I Gram stained it twice to confirm that it was a pure Gram positive coccus. Once it was pure I proceeded to do the catalase test, the bacteria produced bubbles, indicating a positive result. From there I was able to narrow down my unknown to a possible of 4 bacteria. By analyzing my matrix I was able to determine that by doing an Oxidase test, it would eliminate Micrococcus luteus. However, the oxidase test turned positive, allowing me to confirm that my unknown Gram positive bacteria was Micrococcus

The lab manual confirmed the experiments findings to be correct and each one were pink colored on the slide under a microscope. If the result had varied from gram negative a reason could include leaving the decolorizer on the slide for one minute instead of ten seconds. Doing this could make the slide appear any color other than pink. The results could also have varied

Frameless shower doors are an exquisite and ageless expansion to any lavatory's stylistic theme. Then again, conventional framed walled in areas additionally convey style and esteem to your home. Which would it be a good idea for you to pick?

the minute, I washed the bacteria with purified water, then filled the bacteria with Gram’s iodine for

The Open Door policy was initiated by the United States in 1899 for the protection of equal privileges among countries trading with China. The Open Door policy was well received in the United States. For more than 40 years, it was the backbone of American foreign policy in East Asia.

Unfortunately, broken glass on the doors of a fireplace are a very common problem if you purchase them from a home center. There are two reasons for this:

I was wondering if there was any way to lock the sliding doors or if the current roommate who lives in the room knows of a way with the right tools to put a lock ( even if it is a combination lock ) on the sliding doors? Also would the house mind if I put a tapestry/ curtains over the sliding door entrance to spruce up the area/ hide the sliding doors?

There are many reasons for knowing the identity of different microbes when presented with them. The reasons include identifying the causative agent behind the disease in a patient, understanding how it can be treated, as well as knowing the microbe that should be used for making the right antibiotics. With this being said, their correct identification is not only significant in a microbiology lab but also in the medical, manufacturing, and pharmaceutical fields. The first test used in the identification of unknown bacteria numbers 3 and 4 was the Gram stain, which was used to differentiate between gram-positive and gram-negative bacteria based on their different cell wall elements. The Gram stain procedure distinguishes between