Beet Cells Lab Report

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The Effect of Temperature on Beet Cell Membranes and Concentration of Betacyanin Released Introduction Membranes are an important feature of plant cells and they act as a barrier that separates the interior of the cell from the external environment (Campbell et al., 2008). Each membrane layer is composed of a phospholipid layer, which is semi-permeable and possesses the ability to control the movement of diffusion. Within the centre of a cell, the vacuole that is responsible for storage and the maintenance of osmotic balance can be located. In this study, we are using beets to study these cellular structures. Beet is a plant whose cells contain a characteristic red pigment, called betacyanin. It is located in the vacuole, which is…show more content…
Originally, the absorbance measured for samples in –5°C and 70°C were 1.700 and 0.845; the concentrations were 425 μm and 180 μm respectively. However, because both of these absorbance readings were greater than the highest absorbance value in our standard curve (Figure 1), a calculation of diluted concentration for these two samples was necessary. Therefore, using the formula Cu = Cd/D where Cu = concentration of undiluted (original) sample, Cd = concentration of diluted sample and D = dilution (which is 1/5 in this experiment), we calculated that the diluted concentration for the sample in 5°C is 85 μm and for the sample in 70°C is 36 μm. As we inserted these data on the graph (Figure 2), concentration of betacyanin for samples in –5°C and 70°C still dominate in comparison to others. This sudden increase of concentration allowed us to understand that the tonoplast membrane is easier to be damage in extreme temperature. Discussion Our original hypothesis about the effect of high temperature on beet cell membrane was correct; as temperature increases, the concentration of betacyanin increases as well. The beet root sample in temperature 70°C had a higher absorbance value and concentration of betacyanin in comparison to other samples due to the membrane damage. The experiment of lipid membranes performed by the University of Osaka concluded a similar result. They found that the membranes were stable at physiological
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