Behavioral Differences Of Heterodera Glycines And Meloidogyne Incognita Infective Juveniles Exposed

This experiment was centered on metabolic and biochemical testing procedures. The rationale of performing these tests was to distinguish six different microbes from one another and to compare how their metabolic and biochemical processes differ from species to species to determine the unknown sample.

The purpose of this lab is to test for enzyme activity, look at enzyme specificity, and how temperature affects enzyme activity.

A two-part study was recently done to show what natural habitat a Pill bug, Armadillidium vulgare, naturally prefers: wet or dry environments along with a light or dark environment. It was hypothesized that a Pill bug would prefer and wet and dark environment based on its natural habitat of soil. For the wet and dry experiment, a coffee filter and soil were placed in each chamber of a double petri dish with one being dampened before being placed in. For the light and dry experiment a light was hung above one chamber of another double-chambered petri dish while the other chamber was covered with aluminum foil, after placing soil in both chambers. An equal number of Pill bugs was placed in each chamber and a study was taken for

5. What species is the intended host for the Baylisascaris nematode and what symptoms does it cause in this host?

Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. These tests may help in determining a particular pathogen’s growth needs.

I certify that the writing in this assignment is my individual work and is my sole intellectual property. It does not contain the ideas or writing of other individuals/authors.

This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.

This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.

Laboratory Tests – All laboratory tests utilize the following reference unless otherwise specified - Pagana & Pagana (2014)

b. Where the treatment arrangement includes the utilization of limited pesticides. The bug control proficient is authorized to apply confined pesticides.

albicans SC5314 were picked from a single colony and used to inoculate 1 mL of YPD broth, which was allowed to grow overnight with agitation at 30°C, 10 ul of which was then put on BHI agar plates with kanamycin (45 mg/mL) and incubated overnight at 30°C. Gravid nematodes maintained on nematode growth medium (NGM) plates seeded with Escherichia coli OP50 at 20 °C were collected by washing and lysed with a bleaching mixture (0.45 mol L−1 NaOH, 2% HOCl). Age synchronous populations of L1-larvae were obtained as described previously.23 Arrested L1s were plated on 10 cm nematode growth medium (NGM) plates seeded with E. coli OP50 and grown at 20°C until they were young adults. Nematodes were added to 10 cm plates containing 20 mL of BHI agar with 45 mg of kanamycin/mL and live C. albicans or heat-killed C. albicans. Candida cells (50 μL) were added to the plates together with 200 μL of PBS to facilitate even dispersion of the microbes. Three separate replicates of nematodes were exposed to these conditions for 4 h at 25°C. After being washed with sterile M9 buffer, nematodes were lysed to extract small RNA for RNAomics assay. Small RNAs were extracted according to the manufacturer's instructions from the mirVanaTM miRNA isolation kit (Ambion) and converted into a double-stranded cDNA library followed by the adaptor ligation. Quality of double-stranded cDNA library compatible with the Applied Biosystems SOLiDTM system for the next-generation high-throughput

List of nematode strains. Strain N2 Bristol was used as wildtype for all experiments (originally developed by Brenner, S., 1974; provided for present use by the Caenorhabditis Genetics Center (CGC). The following strains were also provided by CGC: CX51[dyn-1(ky51)], AM141[rmIs133[Punc-54::Q40::yfp]], and SJ4005[zcIs4[hsp-4::gfp]]. The following strains were generated in the Caldwell Laboratory at The University of Alabama: AM141[rmIs133[Punc-54::Q40::yfp]];CX51[dyn-1(ky51)] and SJ4005[zcIs4[hsp-4::gfp]]; CX51[dyn-1(ky51)]. The methods used to generate novel strains are detailed in below sections of this report.

Our group of three scientists has access to Kettering University’s Applied Biology laboratories equipped with the necessary instrumentation to perform the biological assays needed to test our hypothesis. The team’s combined experience and background in microbiology, chemistry, and biology will play a key role in the process. Between us, we have participated in research through cooperative education and on campus opportunities, as well as completed several upper-level biology and chemistry courses, including Analytical Chemistry, Biochemistry, Molecular/Cellular Biology and Biological Techniques.

Another purpose of this experiment is to stress the importance of knowing the identity of a microorganism. Knowing the species of microorganism present in a sample provides a

In the mid-1950s, the first SCN resistance soybean sources, PI 90763 and PI 84751 and cultivars Ilsoy and Peking, were identified (Ross and Brim 1957). During the last 20 years, more than 120 soybean germplasm with SCN resistance have been reported (Concibido et al. 2004; Vuong et al. 2013). However, most of them present resistance to only one or few SCN races and only eight soybean germplasm including Peking, PI 88788, and PI 437654 have widely been used for breeding programs in the US (Vuong et al. 2013). Understanding of resistance to the nematodes have remained limited because most of the reported resistance QTL have not been cloned. Also, continuous cultivation of soybean cultivars with the same resistance sources may accelerate selection pressure for SCN as well as other nematode species. Therefore, development of soybean cultivars with multiple nematodes resistance would be essential to prevent losses by the nematodes that can occur in the same field. The lack of diversity for SCN, RKN, and RN resistance source in current soybean cultivars still emphasizes the necessity of identifying new resistance genes/QTL. Therefore, continuous germplasm screening to find new germplasm and genes/QTL would be required for successful soybean breeding programs. The identification of new genetic sources with broad spectrum resistance to multiple SCN HG types, RKN and RN would be beneficial for the development of germplasm and varieties to reduce losses from these nematode species (Lee