Biochemical Tests On The Gram Negative And Gram Positive Bacteria

3760 Words Nov 21st, 2014 16 Pages
Microorganisms have the ability to live everywhere and can survive when put together with another not of its kind. Expectations for this experiment would to successfully obtain credible information behind the mixed unknown and be able to isolate both the gram-negative and gram-positive bacterium. In order to obtain the correct test results and to ensure the right gram mixed unknown is identified there had to be a series of eight biochemical tests performed on the gram-negative bacterium and five biochemical tests performed on the gram-positive bacterium.
The sole purpose behind this conduction was having the ability to understand just how the gram-negative and gram-positive bacteria can be distinguished and identified when mixed together into one broth. Understanding the basic characteristics as to why each bacterium has the ability to dye certain colors and retain those colors. The gram-positive bacteria has the ability to retain the crystal violet color and remain purple as for gram-negative bacteria, they cannot hold the purple color and decolorizes when the ethanol hit its. The gram-negative bacterium does have the ability to retain the counterstain, which is a pink to red color. This happens because of the thickness of the peptidoglycan layer, in the gram-positive the layer is 20-80 nm thick and have a multiply amount of layers but for the gram-negative they only have a thin 1-3 nm thick one layered layer (Badon, 2014).
Biochemical tests for the…
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