Bioinorganic Coordination Chemistry Essay

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Bioinorganic Coordination Chemistry

This experiment uses H2TTP made during the last lab and hydrated copper (II) acetate to convert the H2TTP to Cu(TTP). The reaction proceeds by being refluxed for 30 minutes. The final product is a non-iridescent purple color, unlike the vivid, shimmering purple color of the starting H2TTP.

Introduction

Thin layer chromatography, or TLC, is used as a prerequisite for column chromatography. When performing types of chromatography, like TLC, the polarity of solvents is extremely important. This lab uses TLC plates, five solvents (hexane, toluene, ethyl acetate, dichloromethane, and acetone), and small evaporating dishes to determine which solvent would
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3. Add 0.16 g. of hydrated copper (II) acetate, cover with a water condenser and allow to reflux for 30 minutes.

4. Spot the solution on a TLC plate and check with UV light to see if there is any red on or around the spot. Add additional copper (II) acetate and allow to reflux for 10 more minutes if any red is visible.

5. When the reaction is complete, cool in an ice bath for 5-10 minutes then add 50 mL of distilled water.

6. Transfer into a separatory funnel and wash three times with 25 mL of dichloromethane. Collect the bottom layer each time. Discard the top layer properly.

7. Pour the collected solution back into the separatory funnel and add 50 mL of distilled water. Shake. Drain off the bottom layer.

8. Dry the bottom layer using a rotary evaporator.

9. Make five TLC plates spotted on a line drawn near the bottom, once with the beginning product and once with the final product. Dissolve in dichloromethane to make the spots. Put one of each into the five solvents: hexane, toluene, ethyl acetate, dichloromethane, and acetone.

10. Dilute the product once again with dichloromethane and filter with a pipet stuffed with cotton. Make sure the product is a very light pink color; it will need to be
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