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Bioleaching of Gold Ore

Decent Essays

RESEARCH ART I C L E
Insights intothe dynamics of bacterial communities during chalcopyrite bioleaching
Zhiguo He1,2, Fengling Gao1,2, Jiancun Zhao1,2, Yuehua Hu1,2 & Guanzhou Qiu1,2
1School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan, China; and 2Key Laboratory of Biometallurgy, Ministry of Education, Changsha, Hunan, China
Correspondence: Zhiguo He, School of
Minerals Processing and Bioengineering,
Central South University, Changsha, Hunan
410083, China. Tel./fax: 186 731 88879815; e-mail: zhighe@gmail.com
Received 19 December 2009; revised 14 April
2010; accepted 17 June 2010.
Final version published online 3 August 2010.
DOI:10.1111/j.1574-6941.2010.00943.x
Editor: Alfons Stams …show more content…

Biological
4Fe3þ þ 2H2O
FEMS Microbiol Ecol 74 (2010) 155–164 c 2010 Federation of European Microbiological Societies
Published by Blackwell Publishing Ltd. All rights reserved
MICROBIOLOGY ECOLOGY
Acid generation (reaction 4):
S0 þ 3=2O2 þ H2O !
Biological
2Hþ þ SO2
4
Acid generation (reaction 5):
3Fe3þ þ 2SO2
4 þ 6H2O þMþ2MFe3ðSO4Þ2ðOHÞ6 þ 6Hþ
Denaturing gradient gel electrophoresis (DGGE) has been shown to detect differences in the melting behavior of small
DNA fragments (200–700 bp) that differ by as little as a single base substitution. It is well established that DGGE offers a rapid culture-independent method for detecting and identifying predominant PCR-targeted populations (Casamayor et al.,
2000). DGGE is a powerful and convenient tool for a study such as the present one, which requires sampling of several points and at different times. The objectives of this study were to determine the full extent of microbial diversity of an acid mine drainage (AMD) sample from the Yunfu pyrite mine, and determine as to which population members are the most successful chalcopyrite-bioleaching strains. Firstly, we identified the complete phylogeny of an AMD sample from the
Yunfu sulfidemine by restriction fragment length polymorphism
(RFLP). Then, we monitored the changes in the diversity of microorganisms during the bioleaching of chalcopyrite with biomass collected from the AMD

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