Biologically Essential Proteins : Carbohydrates, Proteins, Lipids, And Nucleic Acids Essay

4. There are other types of reagents used to determine what type of biomolecule a substance is. For example, copper ions present in Benedict’s reagent reacts with the free end of any reducing sugars, such as glucose, when heated. Originally blue in color, these copper ions are reduced by the sugar, and produce an orange-red colored precipitate. Alternatively, iodine-potassium iodide (IKI) may also be used when working with starch. IKI contains special tri-iodine ions which interact with the coiled structure of a starch

1. Benedict’s solution is added to white grape juice and heated. The color changes from blue to orange. Based on this result, what biological molecules are

The purpose of this lab was to test different substances using various procedures to see what biomolecules were present and ultimately find out what restaurant Anna Lyza had eaten at before she died. For the first control test, we used vegetable oil to test for lipids. So, if the solution does not contain lipids, it does not become translucent when placed onto a paper bag square and held up to a light. So, it is a negative result. However, in the presence of lipids, the solution will become translucent when placed onto a paper bag square and held up to a light. Therefore in this case, the result is positive. On the other hand, we used albumin egg to test for proteins in another control test. If the solution does not contain proteins, it will not experience any color change and so it is a negative result. When there are proteins existing in the solution, it will turn bluish/purplish and for this reason it is a positive result. Furthermore in the third control test, we used dextrose to test for simple carbohydrates such as glucose. If the solution does not contain simple carbohydrates, it will not undergo any color change and will remain a blue color. So, it is a negative result in this circumstance. If there are simple carbohydrates present in the solution, the solution will turn reddish and so the result is positive. For the last control test, we used starch solution to test

All living things contain some form of organic macromolecules including: Lipids, proteins, carbohydrates and nucleic acids. All of these organic molecules are alike in the sense that is they are made up of bonded elements such as carbon, hydrogen, oxygen, and to smaller quantities nitrogen, phosphorus and sulfur. The macromolecules each contain large long chains of carbon and hydrogen atoms and often consists of repeating smaller molecules bonded together in a repeating pattern (polymers). To test whether a specific solid white substance is a protein is simple due to the unique chains found in the respective type of macromolecule. To test if the substance is a carbohydrate, two tests could be performed on separate samples of the object. First,

The iodine test is used to identify glycogen and starch. These polysaccharides combine with iodine to

A cell, the building block of all living organisms, is composed of four fundamental biomolecules: proteins, carbohydrates, sugars and lipids. Proteins provide a vast amount of functions cells such as they serve as enzymes, provide structural support to cells, and act as antibodies. Reagents are used to spark a chemical reaction. The reagent used to detect protein traces in a substance is Biuret’s. Biuret’s will turn purple if proteins are present and blue if they are none. Biuret’s copper particles, have a charge of +2, are diminished to a charge of +1 when peptide bonds, which are in proteins, are present, creating the color change. Polysaccharides, which are carbohydrates, are most notably known to provide energy to the body, but they also help in breaking down fatty acids. Iodine is the reagent used to determine whether a substance has starch in it. The iodine/starch complex has energy levels that are only for retaining unmistakable light, giving the complex its extraordinarily dark black-blue shade. If there is no starch found, iodine will remain its natural yellowish-brownish color, but if starch is present, iodine will turn blue-black. Monosaccharides, which are sugars, like polysaccharides, provide the body with energy. To detect monosaccharides, the reagent, Benedict’s, is used. Benedict’s reagent is added to a test tube, then it is placed in

b) Benedict test the solution color will change from blue to pink/orange red, indicating simple sugars are present. Lugols test the solution color will change from yellowish brown to dark purple, indicating starch and polysaccharides are present. Sudan iV test the lipid content will turn into red, indicating lipids are

Proteins are the metabolic workhorses of the cell; they engage in a variety of essential activities ranging from enzymatically catabolizing macromolecular food sources to serving as structural components that maintain cell stability. Maximizing protein function relies on intricate non-covalent interactions occurring on the secondary, tertiary, and quaternary levels that help determine the overall shape of the protein. In their native states, proteins will assume the most energetically favorable configuration. Occasionally however, cells are exposed to exogenous disruptions such as heat stress. Heat Stress can compromise protein three-dimensional structure. Hydrophobic residues tend to be buried in the interior of the protein but when

The Benedict test, is a test to determine whether a solution has reducing or non-reducing sugars. If the Benedict's test is negative, that means there is not reducing sugars. Next, the Lugol's test is to determine if there is starch in a substance. If the solution is yellow or orange after a Lugol's test, that means it is negative. If the results of a Lugol's test is purple or black, then it is

This experiment consisted of setting up a control group of starch in various temperature and then placing both fungal amylases and bacterial amylases in a mixture of starch and placing the solution of amylase and starch in various temperatures of water. After a certain amount of time- different amount of time needs to be used in order to have reliable results- iodine is added in a well on spot plates, then two drops of the mixture of amylase-starch is added from each temperature used, by adding iodine into the plates the mixture will show how much starch was hydrolyzed, this is used to calculate the amount of

For this experiment, we have to prepare our phosphorylase which extracted from a potato. We prepared by weighed about 250 grams of peeled potato and cut it into cubes. The extracts then blended with 100mL of 0.1M NaF. After filtered the contents into a clean 250mL centrifuge bottle, we centrifuged it for 3 minutes. Then, separated the supernatant into a centrifuge bottle, which is our phosphorylase preparation. The enzyme assay used in this experiment today is the iodine test. As the iodine reacts with starch, it will form a brown, blue or black precipitate due to the iodine ions forcing into a linear arrangement. The endpoint of the enzyme reaction indicates the presence of starch by using the iodine test to determine. The faster the endpoint is reached, the less active the phosphorylase is.

In part A, we can identify all of amino acid, Protein and peptide bond by used bond by used to Ninhydrin will detected amino acid by reach with alpha-amino group. In the experiment, all samples are give positive Result except urea because urea is ‘not amino acid or protein.So,the negative result of urea is correct that no change color of product.Glycine,glutamate and methionine give the light purple product. There are positive result show that all of them are amino acid. White cysteine ,Cosein and biuret give the light brown or yellow produch.there are also positive result but the result are different from another because cysteine contain di-sulfide bond. It's make on effect to reaction that can made the light yellow product. Casein is protein that contain a high number of proline residues that made the yellow product also the result of biuret is positive, give brown product from the reaction between ninhydrin and NH-group.For biuret test,alaline copper sulphate reacts with compound two or more peptide bond will give a pink or violet product as the

There are four types of biomolecules, carbohydrates, lipids, proteins, and nucleic acids. Carbohydrates are large chains of sugar found in food and living tissues. This includes sugars, starch, and cellulose. They have the same ratio of hydrogen and oxygen that water has, 2:1. They are broken down to release energy in the animal body. Lipids are any organic compounds that are fatty acids and don’t dissolve in water but do in organic solvents. Fatty acids can be found in natural oils, waxes, and steroids. Proteins are macromolecules that do everything in the cell. They are tools and machines that make things happen. Nucleic acids are long strands of nucleotides, and function primarily in storage and transmission of genetic information. There are two types of nucleic acids, DNA and RNA. DNA is the genetic material of all cellular organisms, and RNA sends out messages from the information that is held in the DNA.

Protein and carbohydrates are both macronutrients required for health reasons. But which one of these macronutrients before a run affects running performance more favorably? Most people recognize protein as something that helps build stronger muscles. But is that the only thing that protein helps with? Carbohydrates which are also called sugar is stored as glycogen in the muscles and comes from healthy (fruit) and unhealthy (candy) places. Researchers were curious to know, what macro-nutrient has a more favorable effect on running, protein or carbohydrate (sugar)? People can improve their running by improving their diet.

Proteins and lipids are major components that construct the human body. From a microscopic standpoint, proteins and lipids take on multiple roles to maintain homeostasis in the body as enzymes, substance transporters, and metabolic regulators. When a protein or lipid has the appropriate number of peptide chains or fatty acids that allow it to retain its structure and carry out its function properly, it is considered a healthy. These are highly abundant in animal-based foods such as fat-free or low-fat milk, lean meats, eggs, and fish, as recommended by the 2010 Dietary Guidelines for Americans (McGuire & Beerman, 2013). In people who have food allergies, these food sources are unhealthy because the proteins in them elicit minor or