Biology 1 Lab Report Essays

2761 WordsMay 14, 201312 Pages
Hydrolysis of starch for fungal amylase Aspergillus Oryzae and bacterial amylase Bacillus Licheniformis at different temperatures. Maydelis Perez PI 4593042 Florida International University BSC 1010L, section UO9, March 6, 2013 Abstract Enzymes are very specific protein because they contain one active site on their surface that enable the substrate to bind to the enzyme and form the enzyme substrate complex and then release the products. The principal function of enzymes is to increase the rate of the chemical reactions. Enzymes have a set of conditions at which they work perfectly; this is known as optimal condition. Fungal and bacterial amylase are the…show more content…
Fungal Aspergillus oryzae and bacterial Bacillus licheniformis amylase are the two types of enzymes that will be used during this lab to test the ability of amylase to break down starch ,a polysaccharide present in our daily diet, into simpler sugars , maltose (Smith,et al,2012). Bacterial amylase is found in different habits for example in human saliva, for this reason is easy to infer that bacterial amylase would break down the starch better and faster than the fungal amylase. According to this information amylase activity going to change higher or lower according to the temperature but it will be faster in bacterial than in fungal, 100% hydrolysis going to occur between 40°c to 60°c because this is the habitat temperature for these species and this means that is the optimal temperature, but in bacterial occur in less time than in fungal because are found in human saliva were chemical digestion begins. Methods The principal objective of this study was to determine how temperature affects the activity of amylase enzyme and the optimal temperature for fungal and bacterial amylase. In order to make this experiment possible we needed fungal Aspergillus oryzae amylase and bacterial Bacillus licheniformis amylase; 5 ml of 1.5% starch were placed in one test tube
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