Biology Essay

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Lab Report 1                              Principles of Biology 1(BIOL 100) Fall 2001                              Gerard Chretien                          Living cells perform a multitude of chemical reactions very rapidly because of the participation of enzymes. Enzymes are biological catalysts, compounds that speed up…show more content…
The temperature is so high that the active site of the enzyme changes and it is unable to bond with substrates. Part I: Effect of Temperature on Enzyme Activity(Invertase) The rate of an enzyme-catalyzed reaction will increase with temperature to maximum rate and then sharply decrease. The rate increases to about 40 degrees Celsius, at which point the structure of proteins is disrupted (denaturation), resulting in an abrupt loss of enzyme activity. PROCEDURE: 1. Number 3 test tubes (1,2, and 3) with a permanent marker. Add 4 ml of a sucrose solution to each test tube. 2. Place tube 1 in a beaker of ice water; place tube 2 in a beaker of water at room temperature, and tube 3 in a beaker of boiling water. Wait 10 minutes for the sucrose solution in the tubes to equilibrate with the temperature of the water in the beakers. 3. After 10 minutes, add 0.5ml of 25 percent invertase (an enzyme derived from yeast) solution to each tube (swirl the invertase solution before use). Cover each tube and invert it several times to mix the contents during the 5 minutes. 4. After 5 minutes add 1ml of Benedict’s solution to each test tube. Place the 3 tubes at the same time in a boiling water for 5 minutes. 5. Remove all 3 tubes from the water bath at the same time and observe the amount of precipitate and the color of the Benedict’s solution in the tube. Then I would record my observation on a data graph sheet

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