Biology Lab Report

Corporation, W. B. (2013). Introduction to Enzymes. Retrieved 2013 йил 27-2 from Worthington Biochemical Corporation: https://gateway.emmanuel.qld.edu.au/cvpn/aHR0cDovL3d3dy53b3J0aGluZ3Rvbi1iaW9jaGVtLmNvbQ/introbiochem/effectsph.html

Plant tissue culture alludes to the In vitro culture of plants from plant parts (tissues, organs, developing lives, single cells, protoplasts, and so forth.) on supplement media under aseptic conditions (Altman, 2000). In vitro cultures are presently being utilized as apparatuses for the investigation of different essential issues in plant sciences. It is presently conceivable to engender all plants of financial significance in vast numbers by tissue

Everyday society passes by minute details with no regard what so ever for the magnificent organism that functions to produce an essential gas vital for the human life. This magnificent organism are the various leaves one may find in backyards to parks to school. Although they are small and disregarded, they have various forms that are each unique to certain regions. Therefore the purpose of the lab is to explore the different types of leaves in different environment with different climates. Its significance is that we individual can observe how various factors affect the leaves in a specific area. The experiment will take place at Fullerton’s local university, California State University Fullerton. They possess an incredible arboretum that

Engineered s. cerevisiae with a reconstituted 7-gene pathway is feasible for the synthesis and production of codeine and morphine from (R)-reticuline (Fossati, et.al, 2015). S. cerevisiae cells fed “(R)-reticuline, salutaridine or codeine as substrates showed that all enzymes were functionally co-expressed in yeast and that activity of salutaridine reductase and codeine-O-demethylase likely limit flux to morphine synthesis” (Fossati, et.al, 2015). This study describes a significant advance for S. cerevisiae and paves the way for complete synthesis of substances not naturally produced by microbes. Morphine alkaloids are narcotic analgesics and the most powerful naturally produced alkaloids used to treat severe and chronic pain.

The free hydroxyl group of galactose at 6th carbon is essential for galectin binding49.Another very significant structural feature of ST6Gal-I is that it contains six cysteine residues which are also involving disulfide linkages, Cys142-Cys406, Cys184-Cys335, and Cys353-Cys364. These disulfide bonds give important features such as hydrophobicity and rigidity. The other major structural feature of this enzyme is containing a large glycan (with ten monosaccharide units) covalently bonded to Asn149. This specific glycan is also the acceptor for the reaction catalyze by ST6Gal-I. Therefore, The ST6Gal-I is acting as an enzyme as well as a

Similar to thioredoxins, glutaredoxins (Grx) are small antioxidative proteins that use glutathione and NADPH for their regeneration. In addition to this, recent studies have shown that some classes of Grx can bind iron in Fe-S clusters and thus reducing the availability of iron in the Fenton and Fenton-like reactions [108]. Although Grx and Trx are very similar in their structure and mode of action, only Grx possess dehydroascorbate-reductase activity (DHAR) and can take part in the regeneration of an important cellular antioxidants - vitamin C [108]. Since Grx expression was opposite to Trx expression in O. nubilalis (Figure 18), this indicates spatial adjustments of the antioxidative defense systems in this species, in order to optimize the

Nearly 50 years after the first lysozyme crystal structure had been published, the missing piece, the SN2 reaction of the glycosyl-enzyme intermediate, was found. The first lysozyme structure was that of a hen egg white, and it provided a deeper understanding of the mechanism of enzyme reactions. Lysozyme enables the transfer of a glycosyl group to water to occur more quickly. The cleavage of the C-O bond in glycosides without the lysozyme occurs rather slowly.

This report will be analysing the effectiveness of Gibberellic Acid on different plants to see if it helps them germinate faster. This experiment will be looking at the plant types, Raphanus sativus (Radish), and Echinocactus grusonii (Cactus). It aims to see if the Gibberellic Acid increases the rate at which seeds germinate. Then, if the Gibberellic Acid does, this experiment will look at what ppm ration of GA (Gibberellic acid) results in the fastest germination time. The hypothesis being explored, states, “The seeds containing a 500ppm of Gibberellic Acid will germinate faster than one with more or less.”

On Plate I (LB) and Plate II (LB + Ampicilin), E.coli growth was expected to occur. Since the environment was not selective in Plate I, growth was expected to be found as lawn. This prediction was confirmed by the experiment. On Plate II, it was expected that either lawn or colonies would be found. The observed growth occured as lawn. On Plate III (YPD) growth was predicted, given the presence of all the nutrients that the auxotrophic yeasts cannot synthetize. Due to the usual non-selectivity of the medium, lawn was the expected type of growth. However, growth actually manifested as red colonies, an occurrence which will be explained in the Discussion section. On Plate IV (YNB, -AA) and Plate V (YNB, -AA, +5% AS) no growth was expected to happen given the absence of amino acids, which the auxotrophic yeast could not synthetize. The results were as predicted. On Plate VI (YNB, +AA-Arg, -AS ), it was expected to obtain colonies, due to the selectivity of the environment. This indeed was observed, in the form of white colonies.

Overall, the results suggest that, this was somewhat of an effective method in isolation the enzyme GAPDH from yeast

Thioredoxins (TRX) are small proteins (around 12 KDa) which are present in all organisms. In addition to these ‘classical’ thioredoxins, numerous proteins also exhibit thioredoxin like domains or multiple thioredoxin domains (P., 2001). Thioredoxin is a general name for small proteins and conserved WC(G/P)PC motif to catalyze thiol disulfide interchanges (Meyer, Buchanan, Vignols, & Reichheld, 2009). Trx proteins function in disulfide reductases to reduce H2 O2 to non-reactive H2O and to scavenge free radicals, This protects cells against oxidative stress and apoptosis (Wang et al., 2013). A large number of Trx proteins have been identified in plants(Meyer, Reichheld, & Vignols, 2005).There are six different types (m, f, x,

Genetically modified crops are produced using a process called ‘genetic engineering’. Using the process of genetic engineering enables to add a different gene into the target plants’ cells. It then alternates the plants’ genetic content to make new characteristics and give additional abilities such as adding more proteins and vitamins. The first method of genetically modifying a crop involves identifying and copying the gene of the donor organism with a desired trait, this procedure is called mapping. The second method involves transferring the gene into the target plant and when it is injected into the target plants’ cell; it then becomes part of the cells genetic characteristic. To be able to transfer the gene into the target plant, two

To date, FragariaCyc is the only resource that provides a comprehensive knowledgebase of strawberry metabolism and allows users to upload and analyze data of their choice within the framework of cellular-level metabolic network supported by extensive manual curation. FragariaCyc is based on the sequenced genome of F. vesca. version 1.0 (Shulaev et al., 2011) and shares functionalities with other BioCyc pathway databases including several species-specific plant pathway databases (Mueller et al., 2003;Urbanczyk-Wochniak and Sumner, 2007;Dharmawardhana et al., 2013;Monaco et al., 2013;Naithani et al., 2014) available from http://pathways.cgrb.oregonstate.edu.

Once the plants go through abiotic stresses like drought, temperature, and salinity, ABA is produced to rescue the plants from these harsh conditions. ABA evokes signal transduction pathway leading to the activation of the members of SnRK2s, namely SnRK2.2, SnRK2.3 and SnRk2.6 (Fujita et al., 2013). The SnRK2s leads the activation of downstream AREBs/ABFs. SnRK2s are responsible for ABA-dependent phosphorylation of AREBs/ABFs in multiple phosphorylation sites (RXXS/T) in protein sequence (Figure 2) (Nicolas et al., 2014). AREB1/ABF2, AREB2/ABF4, ABF3, and ABF1 are the major TFs which are downstream of ABA-activated SnRK2s in ABA signalling pathway during vegetative growth.

The evolution of ACC also brings about the production of 5¢-methylthioadenosine-nine. Increase in the rate of respiration of the fruit gives ATP (Adenosine triphosphate) need for the methionine cycle and can lead to induced ethylene production without high levels of intracellular methionine. SAM is a crucial methyl giver and relates to numerous aspects of cellular metabolism. Thus, the two steps involved in the synthesis of ethylene is the formation of ACC and its conversion into ethylene. The genes encoding ACS and ACO have therefore been studied more deeply than other enzymes in the pathway, although there is proof that a few other genes concerned with methionine synthesis and methionine salvage pathway are differentially demonstrated throughout ripening and in response to ethylene (Alba and others 2005; Zegzouti and others 1999). ACS and ACO are encoded by multigene families in higher plants, with tomato having at most nine ACS (LEACS1A, LEACS1B, and LEACS2-8) and five ACO (LEACO1-5) genes (Barry and others 1996; Nakatsuka and others 1998; Oetiker and others 1997; Van-der-Hoeven and others 2002; Zarembinski and Theologis 1994). Expression survey has disclosed that at least four ACS (LEACS1A, LEACS2, LEACS4, LEACS6) and three ACO (LEACO1, LEACO3, LEACO4) genes are differentially shown in tomato fruit (Barry and others 1996, 2000; Nakatsuka and others 1998; Rottmann and others 1991). LEACO1, LEACO3, andLEACO4 are shown at low levels in green fruits that are in the first