Biology Lab Report

797 Words4 Pages
An association between enzyme production, gene copy number, and gene evolution was explored by conducting analysis of the salivary amylase enzyme, AMY1A gene copy number, and the ancestral starch consumption in Homo Sapiens (Tracey 2017, p.22). It was hypothesized that the relative amount of starch consumption was very high for my personal ancestral diet, thus my AMY1 diploid gene copy number in my genome and salivary amylase concentration would be significantly higher than the population mean. With a population of 28 subjects (n=28), individual saliva samples were collected and compared to a calibration curve to determine the approximate amylase concentration by analyzing absorbance values. Individual samples of buccal cheek cells were…show more content…
Specifically, alpha-amylase is produced by the salivary glands of Homo sapiens (Humans) as well as many other mammalian species and is encoded by the gene AMY1A (Tracey 2017, p.22). The enzyme alpha-amylase is able to uptake polysaccharides including starch and glycogen as a substrate then hydrolyze the alpha-1,4-glycosidic linkages that connect the monosaccharides together (Tracey 2017, p.37). This is the reason as to why salivary amylase is also referred to as alpha-amylase (Tracey 2017, p.22). The salivary amylase gene has undergone duplication over time, and DNA hybridization studies have revealed that individuals have a varying number tandem repeats of the AMY1A gene (Tracey 2017, p.22). In Perry et al.’s (2007) summary article reviewing the role of selective pressures on AMY1 gene copy number and amylase concentration, they found a moderate positive correlation between AMY1 gene copy number and salivary amylase concentrations in individuals. The possibility that different selective pressures in populations have affected amylase production was stated (Perry et al. 2007). Copy numbers of the salivary amylase gene was positively correlated with salivary amylase enzyme concentration, thus individuals with apparent evolutionary exposure to high starch diets had, on average, more AMY1 gene copies than individuals from backgrounds with relatively low starch diets (Perry

More about Biology Lab Report

Open Document