Biology ia

Rubbing alcohol, also known as isopropyl alcohol “refers to two water-soluble chemical compounds—ethyl alcohol and isopropyl alcohol—that have generally underrated germicidal characteristics” (“Guideline for Disinfection and Sterilization in Healthcare Facilities”). Ethyl alcohol kills bacteria in two ways: “protein denaturation and dissolving the lipid membrane” (“How Does Ethyl Alcohol Kill Bacteria?“). Next, Betadine, also known as Povidone-iodine is a solution [that is] often used in hand scrubs and hand rubs in hospital settings” (Ngan). It is used in “Treating minor wounds and infections, as well as killing bacteria” (“Povidone/ iodine solution”). Povidone-iodine’s way of killing bacteria is still being researched and “is yet to be fully understood, [but] it appears to have the ability to penetrate the cell wall of bacteria and affect their structure and function. This ensures their rapid destruction and helps protect against wound and skin infection” (“What is an Antiseptic”). Germ-X Hand Sanitizer is a hand gel that is used for waterless “washing” of hands to kill germs on the surface. It is an antiseptic primarily used by those who want to kill germs quickly and cannot wash their hands (Samuels). The formula contains about 63 percent ethyl alcohol according to livestrong.com (Samuels). Neosporin is a topical antibiotic used to

The hypothesis behind this experiment is that the Gibberellic acid has a positive growth effect on the plant and causes it grow larger in height.

The active ingredient in Dettol Antiseptic Liquid is 4.8% chloroxylenol, a chemical that inhibits the growth of a large range of microorganisms including gram negative bacteria. It can be used to cleanse flesh wounds or disinfect surfaces. Chloroxylenol destroys the

The results observed do not correspond with the outcome predicted by the hypothesis. Despite the nature of the subjects of the experiments, no substantial growth was observed. Only one seed of the 36 planted germinated, and it could only survive for a period of a week. The one seed that germinated reach a height of 1.2 cm. Table 1 presents the average growth observed in each quad. Each quad had a total of 12 seeds. No seeds were removed during the course of the experiment.

“Ajax Spray ‘n’ Wipe” specifically states that it kills E. coli. This is because of the lactic acid in the spray, which is used as a natural antibacterial agent.” ("Colgate-Palmolive | Product Ingredients"). Lactic acid kills unwanted bacteria especially in food which is where E. coli is often found and transmitted, of which meat is the most common cause. Vinegar was chosen as a substance because “it can be used as a bleach alternative” (“Disinfectants: A Guide to Killing Germs the Right Way”) and bleach kills E. coli. It is also used as an everyday cleaning product. The last substance chosen was salty water because it is thought that salt kills bacteria. Much of the world’s population has access to salt and if the experiment proves that salt kills E. coli it would be a helpful factor for further treatment of the

Out of the all the antiseptics being used in this investigation the more well known antiseptics such as dettol and sudocrem will be more effective when stopping the growth of bacteria than natural antiseptics like lemon.

Although the experiment was as fair as it could have been, there were some factors that were beyond our control. Firstly, the tubers that we used may not have been from the same specimen,

The objective of this experiment was to identify two unknown bacteria from a mixed culture. Which was done by using the aseptic technique which was very important to avoid any contamination and keeping the workspace clean while culturing bacteria for different tests. To start, I chose a tube which had a solution of mixed culture. I used the flame to sterilize the inoculating loop and dipped it into the tube and streaked for isolation on 2 TSA plates and placed them in an incubator at 37 for 24 hours. Next day I observed the growth of 2 different types of colonies one for each unknown on the two plates. So I picked the best one and labeled it as master plate and discarded the other plate. From the master plate, I subcultured each type of colony

In the future, this experiment could improved or expanded. One area of improvement could be to do this experiment several times and then averaged together or compare the results. This leaves less room for error and could make the data more accurate and precise.

One MR-VP broth was the control tube, which was not inoculated at all. The other MR-VP broth was the treatment broth that was inoculated with the colony 2 stock culture at 30 degrees Celsius for five days. The reason for completing this test first after gram staining was because it was a 5-day test that could be left over the weekend. There were no variables, it was either negative or positive, and there would be no misleading guesses. The plan I had going into this was to perform all the tests possible with no variables or not determined results to reduce the potential for errors. On Monday I will get the control and treatment inoculated test tubes back to determine wither the unknown organism is positive or negative based off of the color in which the broth will

At this point in the experiment, we labeled two Luria Bertani nutrient-rich agar plates with the strain name, group initials, date, and which dilution it was. From there we were able to pipet 100 µL of our final dilution onto one of the nutrient-rich L medium agar plates. After 100 µL of our dilution was placed in the middle of the plate, we flamed the L-shaped spreader by dipping it into 95% ethanol and then placing it over a Bunsen Burner flame. My lab partner and I waited for the flame to go out and cool off before placing it on our bacteria. After letting it cool, we were able to move the L-spreader onto the bacteria and spread the bacteria all over the agar plate. After spreading the bacteria, the L-spreader was then sterilized again before putting it down on the

During this experiment many errors were made. The biggest error that was made was that we did not control the environment that the slime molds were in. We initially kept them at school in favourable conditions, where growth was good. We then took them home and moved them around the house, so the slime mold was constantly having to adapt to these environmental changes, until it could not withstand it anymore, and eventually died. Another error that was made was the fact that in transferring the slime molds, and feeding them we used only inoculating hoops, these inoculating

We hypothesized that at Miracle-Gro concentration 1, this solution will be the most effective towards the rate of growth because at pure solution, the concentration is so high that the effect will occur sooner than those of lower concentrations, and speed up the life span to expiry of all the plants in the single solution concentration. Because we accept as true, that pure concentration will have the highest rate of growth, we can eliminate this concentration from our second question. We have come up with the presumption that at the concentration of 0.1 solution will allow the plants to be the most successful for the longest period because concentrations less than 0.1 would not appear to have any effect. There would be limiting nutrients within the solution and therefore would have a yield due to the lack of Miracle-Gro

Natalie notices that the average diameter of batch 17 is extremely low compared to the average diameters of the other batches. Our recommendation to Natalie is that she should check what happened on Day 17 to find the reasons for these out-of-control-results. As the next step, Natalie should take the required actions for solving the discovered problem.

This experiment was performed to test the hypothesis if LB nutrient broth, +pGLO and -pGLO Ampicillin, and Arabinose was placed in the E. coli plates, then there will be a significant growth in the newly transformed bacteria and it will possess the ability to glow under UV light. The measurements were recorded from the bent glass tube in each glass test tube. The transformation protocol tested for the newly possessed traits in E.coli bacteria. Throughout the experiment there were many probable reasons for failure. If the pipettes and sterile loop were not thrown out in between each use, a cross contamination could cause a miscalculation in the experiment causing the data results to fail. The hypothesis that was tested was validated due to the positive results with each experiment stating that newly transformed organisms due in fact pass on traits.