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Boiled Peas Lab Report

Decent Essays
Image 3.1 & 3.3-Raw and Boiled Peas is a Tetrazolium Solution
As we discussed during the experiment, the raw peas are not denatured therefore, containing proteins still. But, the boiled peas it appears to show that they are red, suggesting that they are denatured and also lost their functionality. Tetrazolium was able to react with the raw peas that contained proteins. However, it did not react with the boiled peas because of their denaturation.
The next image shows different solution; blank being the buffer solution also containing Tetrazolium. Test tube A contains pallet one suspension with the Tetrazolium, test tube B contains supernatant one and Tetrazolium, test tube C contains supernatant two and Tetrazolium and finally, test tube D
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At the bottom of the test tube there is sucrose, which is transparent. The darker shade of green is the unbroken chloroplast cells and the light green is broken chloroplast mixed in with the sucrose gradient.

Table 3.4 IKI Results
Sample Observations
Gunk Empty boxes (cell walls), live cell with nucleus orange/red brown colour
Pellet 1 Lots of nuclei, debris that is large, visible purple starch granules
Pellet 2 Mitochondria as lines nuclei that are brown purple starch granules

Image 3.3- Gunk No Iodine (left) and Iodine (right) Magnification 40X
Live cell walls are on the left does not contain iodine, and the right image contains iodine therefore they are dead looking.

Image 3.4- Pallet 1 No Iodine (left) and Iodine (right) Magnification
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The gunk contained many fibrous cells, these cells were from the broken down cells. Also it contained debris in the sample from the dirt, but did not see other organelles. In pallet one, as a group we were able to see nuclei of cells that were the brown spots in the diagram. Starch was also seen which was in purple, and we continued to see more debris. The first pallet is referred as the nuclear palette since it contained a vast amount of nuclei. The last palette contained starch particles that were coloured in dye, and also contained mitochondria, which were the small dots and lines. The objects, organelles and other structures that were observed within the supernatant 2 sample were mainly ribosomes because once the sample was centrifuged they were segregated and brought to the top of the
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