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Bovine Serum Albumin

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b. Peptide Conjugation to Bovine Serum Albumin Described by Coligan et al (2005), Glutaraldehyde coupling was needed to conjugate the two peptides individually to bovine serum albumin. BSA dissolved in 500ul of 0.1M borate buffer pH10 was manipulated to mix with the air-oxidized peptide solution that was already prepared taking note that the mixture has to be on a 1:10peptide-BSA ratio. Moreover, until a 1:10:1 peptide-BSA-glutaraldehyde ratio was achieved, the peptide-BSA mixture was combined with 1% glutaraldehyde. Appendix B includes the computations for the preparation of antigen conjugation. After, the mixture was incubated for 2 hours at room temperature and was diluted with distilled water until a 1mg of peptide in 1ml of the peptide-BSA-glutaraldehyde
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