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Breast Cancer Essay

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Breast cancer exists today as a vicious disease which has multiple facets for killing its host. The largest indicator of survival rate is length of time alive after diagnosis with the disease, and then whether or not the disease has metastasized to a new location in the body. For breast cancer patients, the 5-year survival rate of patients below Stage 4 is 90%. The stages below the 4 stage represent the stages where the breast cancer has not metastasized into other regions of the body. When breast cancer metastasizes into another region of the body, the 5-year survival rate of these individuals drops to 22%. The most common places of metastasis include the brain, liver, and lungs. The goal of studying cell line 231BR is that of …show more content…

Enrichment would not work for this type of analysis as it does not separate the targeted analyte, rather it raises the concentration of the analyte that is involved. Knowing that purification is the type of separation that is to be used, the analyte has to be prepared for analysis by permethylating the glycans preemptively for HPLC, LC-MS, or HILIC. Each respectively stands for High-Power Liquid Chromatography, Liquid Chromatography/Mass Spectrometry, or hydrophilic interaction chromatography. The most common to use is LC-MS as the analyte of interest can be separated and purified for mass spectrometric analysis. Permethylation to understand is a common derivatization method for enhancing ionization efficiency in MS analysis and improves the overall structural stability of the glycan analyte. Later once the analyte is permethylated it is allowed to run through liquid chromatography which separates the analytes by intensity (quantity) and size, which gives the ability to select the analyte of interest. The selection is done with knowledge of what the analyte of interest is before starting the procedure. After the analyte of interest is purified through LC (liquid chromatography) it is taken over to the mass spectrometry machine where the sample is then broken down into the various components. The various components are then broken off one by one slowly until the

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