Results
Samples of Artemia eggs were exposed during three days to four different salinities: 0%, 0.5%, 1% and 1.5 %. After gathering all the results, one may observe the level of salinity that contributed to the larger hatching success of Artemia. The sample exposed to a salinity of 0.5% exhibited the greatest hatching whereas the sample exposed to the solution with no salt, or 0% salinity, did not show any hatching nor partial hatching (Table 1). The sample exposed to a 1.5% salinity presented some free-swimming individuals and partial hatch eggs, but they were less than the ones exposed to 0.5% (Table 1). The sample containing 1% salinity was lost during the experiment and we were not able to record the data (Table 1).By looking at the recorded data of the overall class (Table 2), one may still perceive that the most effective solution for hatching was 0.5%
Table 1: Results of Artemia Hatching Experiment; Number of Eggs by Category in One Trial
Salinity Percentage 0% 0.50% 1% 1.50%
Initial egg count 16 26
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It was predicted that Artemia populations would not survive in waters containing high percentages of salinities since t would cause the cell to shrivel and probably die due to dehydration. For this reason, it was hypothesized that the eggs in the solution with the highest salt concentration would not be able to hatch. During the experiment, the data for the 1% salinity was lost when the petri dish with the solution and the specimen accidentally dropped. However, our hypothesis was not supported by the recorded data. The results show a major amount of hatched eggs at 0.5% salinity, while the 1.5% salinity presented a lower amount of hatching. However, hatching was not present in the solution with 0% salinity. Therefore, I reject my
If hatching viability is affected by the amount of motor oil in the environment of the brine shrimp eggs, then when the amount of motor oil is increased the hatching viability will decrease.
Brine Shrimp are a micro-crustacean that is found mostly in inland saltwater bodies, however, the salinity levels that they live in vary greatly. The conditions that they require can be made at home as the eggs (cysts) of the shrimp are dry, hard shells that can withstand drought and go without water for up to 50 years. This study presents the results of 3 different salinity levelled environments to the Brine shrimp in order to discover a salinity level that produces the most successful hatching rate. By filling 3 Petri dishes with 20mL’s of purified water, each dish contained varying sodium chloride amounts from 15ppt, 25ppt, and
The purpose of this lab is to determine what is the concentration of the unknown solution along with what concentration would be more isotonic to the chicken egg?
Osmosis took place in the egg under a variety of conditions because the membrane of the egg has tiny openings where water can pass through from high to low concentrations. On day one, it was a hypotonic solution because the higher concentration of water in the vinegar moved to the smaller concentration in the egg. This means the egg was filled with water, which resulted in the eggs increase in size. On day three, the hypotonic solution occurred because the corn syrup had a lower concentration of water than the egg. That means the egg released water into the corn syrup, which resulted in the eggs decrease in size. On day four, the egg was placed in water which resulted in an isotonic solution where the concentrations become even. This
The primary purpose of the lab was to test and observe the effects of the process of osmosis on decalcified eggs. Before the process, the initial weights of the eggs were taken and noted. Two eggs were then immersed in a deionized water solution. The same was repeated to the other two solutions of 20% and 40% sucrose. The weights of the eggs were measured after 15 minutes to note the change in mass due to osmosis and again after 60 minutes to take the final measurement. Overall, while some human error might arise in the weighing process, care was taken in handling the eggs and during the weighing process. As such, it was found that the eggs behaved as predicted. It was found that the eggs placed in the hypertonic solution lost
At the conclusion of the experiment, the two hypotheses were reviewed. Because the water temperature did affect the normal respiration patterns of the goldfish, the null hypothesis was disregarded and the alternative hypothesis was accepted. From the results of this experiment, it was concluded that although other environmental factors could play
To approach this problem, eggs of the brine shrimp were placed in 5 different solutions with different salinity. By observing how many eggs hatch per given number of eggs in the solution, the hatching viability was calculated for each solution.
The purpose to this experiment was to study the effect of osmosis in de-shelled chicken eggs in different percentages of sucrose solutions. Osmosis is the process, in which, water moves across a differentially permeable membrane. The eggs were soaked in vinegar to remove the outside hard shell but still leave the egg in its membrane. By placing the six de-shelled in different sucrose solutions, we tested the rate of osmosis. The eggs were placed in the solutions for an hour and weighed in fifteen minute intervals. Then, each weight was recorded and graphed. The results showed that the egg in the water solution gained the most weigh and the only other egg that gained a little weight was the one in the 10% solution. All the other eggs in the different solutions lost weight, even the unknown solution. According to the results the egg that was in the distilled water solution gained weight because it is the hypertonic solution. All the other eggs lost weight because they were placed in hypotonic solutions with different concentrations of sucrose. The egg that was placed in the higher concentration of sucrose lost the most weight. So, the higher the concentration of sucrose, the more water the egg lost.
The barnacles were tested using a small cup filled with water which has a small rock with barnacles on it. Before the experiment was conducted, the barnacles were fed food. The change in cirri beats over one minute was determined. The average decrease of cirri beats per minute was 12.6. Although the manipulated trial was inconsistent, it is still logical to conclude that the
Artemia salina are used as environmental indicator species. They are used to test changes in their environment. A. salina is sensitive to toxins, temperature, and drugs. For the experiment, cysts were put into different amounts of ethanol alcohol and their viability was measured. The hypothesis used was if there is more ethanol alcohol, then the viability of the brine shrimp is unfavorable. The brine shrimp were put into sixteen Petri dishes with the same amount of brine solution. Different amounts of ethanol alcohol were added to each Petri dish. After 168 hours, the brine shrimp were
The experiment took place in a laboratory setting, and the first step was obtaining sixty individual Daphnia magna (that were neither adults nor tiny offspring) from a large tank in the lab. These individuals were equally divided into three groups; low density, medium density, and high density. The twenty Daphnia assigned to the low density group were split into four groups of five and pipetted into one of four tubes filled with 10mL of Chlamydomonas algae. The twenty Daphnia assigned to the medium density group were split into two groups of ten and placed into one of two tubes also filled up to 10mL with Chlamydomonas. The final twenty Daphnia were all placed into a single tube filled with 10mL of the algae. In order to avoid suffocation-related
When the 4 eggs were weighed it was found that out of the 4 solutions, 2 were hypotonic and 2 were hypertonic. The mass of 2 of the eggs increased and the other 2 decreased after 60 minutes in the various solutions. Out of the 4, the most hypotonic solution was the distilled water; and the most hypertonic solution was the solution with 2.0M glucose. The
This experiment was designed to determine the relationship between the color of light absorbed by the Lemna and the rate of reproduction. The rate of reproduction was determined by the change in the number of Lemna fronds in the beaker over time. The higher the rate of reproduction, the more effective the color of light used is on increasing the rate of reproduction. {Table 11}- Relationship between the number of Lemna fronds present in the beaker under the white light and the days passed Color of Light Average rate of reproduction of Lemna (Lemna fronds /day) Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Overall Average Overall Standard Deviation
This can be seen in table 2.2 as when there is a greater difference in concentration between the intracellular and extracellular the rate of osmosis is faster, for example when looking at when both eggs were placed in the solution with no sodium chloride the rate of osmosis was the fastest compared to when they were placed in the solution of 2% sodium chloride where the rate is much slower. Both figure 1.2 and figure 1.3 also support my hypothesis as they demonstrate that the highest rate for both was when the eggs were submerged in the solution containing no sodium
After 3 days in the diluted seawater the crab’s haemolymph Sodium levels were significantly lower (p<0.001) than the crabs stored in full strength seawater as seen in figure 3. There was no significant difference between the mean haemolymph Sodium values of the three dilutions at 75%, 50% and 25%.