Brittany Caruana Essay

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IMMUNOASSY TECHNIQUES APPLIED FOR DECTECTION OF CATTLE IMMUNE RESPONSE TO ANTIGENS FROM FASCIOLA HEPATICA (LIVER FLUKE) INFECTIONS Alysia Kovos 18086284 Demonstrator: Brittany Caruana INTRODUCTION The parasite Fasciola hepatica (liver fluke) is a leaf like flatworm that is a part of the phylum Platyhelminthes (Grove, 2003). F.hepatica are hermaphrodites enabling all of them to asexually reproduce however they tend to mate with each to ensure genetic diversity and can have many eggs in a day (Grove, 2003). The eggs can survive for up to 6 months in dung until they hatch as miracidium when temperatures are favourable (Lenton and Behm, 2000). These miracidium will move through a moist wet habitat to find and enter aquatic snails or fish as these…show more content…
From this previous study it also supported the results in that serology ELISA method is unable to detect the intensity of infection from liver fluke in a host (Charlier et al., 2008).For this experiment the cattle were purposely infected with F.hepatica to analyse for the antibody levels on days 0, 28, 42, 56, 70, 89, 90. From the figure 2 it shows that from day 0 to day 70 during infection there was a steady increase of antibody production. With an OD value of 0.01 on day 0 this is the only day that was below the cut off value at 0.03 suggesting that on day 0 the individuals weren’t yet infected. At day 70 was when the highest antibody count was recorded at 0.56 OD, by day 89 the antibody OD had dropped to 0.47. Suggesting that day 70 was the when most the liver fluke had been killed off and therefore day 89 was showing a decline in antibody count parallel to the reducing numbers of liver fluke present. Day 98 was the post infection day where there was no more liver fluke present however the OD value of 0.53 showed an increase from day 89 this may be due to the body preparing in case of a second exposure to this particular antigen present in the liver
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