Business Level 3 Unit 9 M1

In step 4e, pour the solution into the "+air" bottle and cap it, placing the open end of the collection tube inside the gas collecting

tube was placed inside, then another test tube with an equal amount of substance would be placed

During the heating period, a noticeable change took place in the sugar test tube – it began to caramelize, melting into a golden brown color, then finally a dark brownish/black hue; it remained constant at that color. The copper sulfate began losing color when applied to the heat, turning from a bright blue to white and remained white after heating. Once cooled, 2-3 drops of water were added to each tube; the sugar remained the same, while the copper sulfate immediately bubbled and transformed from white back to its original blue hue. Next 1-3 grams of the hydrated copper sulfate were added to a crucible, which was weighed prior to adding the sample, then heated gently with a Bunsen burner. Once the salt stopped changing color, it was heated for an additional 5 minutes, then the total mass was measured. Finally, a pea-sized amount of Epsom Salt was added to a test tube and heated for 1 minute without the tube becoming red

Setup the 6 test tubes in the rack, place a little crystals of NaCl, KI and benzoic acid. Add 10 drops of cyclohexane, toluene, and water.

Now rinse the conical flask thrice with deionised water by pumping the water around neck of the flask and swirling it. Then tip out the waste.

Add 5 mL of 0.33 M H2C2O4 chemical into the second set of 150-mm test tubes using a pipet.

Attach the clamp to the apparatus, making sure that the end of the tube is 2 cm off the desk.

First we added 4 mL of HNO3 into a beaker. After the substance dissolved, distilled water was added until the beaker the beaker was half full. The mixture was then stirred into the distilled water. Add 30 mL of NaOH into the solution, and stir. The solution was then heated slow. As the product was heating slowly it was stirred. A thermoter was placed on a ringstand and clamp and then placed inside the solution.The solution was heated until 61 degrees celsius. The beaker was then removed from the heat and left to settle. 200 mL of distilled water was placed inside a 400 mL beaker and put on the heater. When the CuO settled for 10 minutes, the solution was then placed inside of a funnel. After the solution is drained, the copper is then washed away with the distilled water. The soultion was tested with a litmius paper. 15 mL of 6.0 mL of H2So4 into the solution. Next the Zinc must be added, then observe the reaction. Then add 10 mL of 6 M Hcl to the Cu. Pour out the Hcl, then add water to the copper and rinse. Then get a beaker and add Cu to water and apply heat.

Using a Pasteur pipet, add three drops of 0.05M KMnO4 to test tubes 1 and 3 and add two drops of 1% FeCl3 solution to test tubes 2 and 4. Record your observations.

The experimenter should make sure to stir the solution thoroughly use the measuring beaker to add the correct amount of

While waiting, place the funnel in a dry graduated cylinder, and place two cheesecloth meshes on top of the funnel so that it can filter the applesauce from the liquid produced.

Carefully place the filter column on top of another clear plastic cup. Slowly pour half of the water mixture into the filter. The water should move between each section of the filter drop by drop. Meanwhile, continue to mix the contaminated sample with a spoon so the dirty doesn’t settle at the bottom.

D. The goal here is to create a vacuum within the plunger as a means of drawing the air from the leaf tissue. The vacuum is created by holding a finger over the narrow opening of the syringe while simultaneously drawing back the plunger. Hold this vacuum for roughly 10 seconds. While holding the vacuum, swirl the leaf disks to suspend them in the solution. Release the vacuum by letting the plunger spring back.

2. Place 1250 ml beaker containing 75 ml HCl underneath the buret (buret already set up with the volume recorded) for titration and apparatus.

Aspirate all wells, then wash plates four times using Wash Buffer (1X) and set fill volume at 350 μL/well/wash. After the final wash, invert plate and blot dry by hitting plate onto absorbent paper or paper towels until no moisture