C. Elegans Lab Report

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

Caenorhabditis elegans, a type of free-living nematode that is found in soil, is the subject matter for this experiment (Marsh). C. elegans allow for an exceptional model organism because it is easily raised in the laboratory setting, it reproduces rapidly, has a short life cycle of 3 days where it develops from egg to adult worm, only 1.3mm in length, and although only having 959 somatic cells, it shares many characteristics with other multicellular organisms like having organs and a nervous system (Kaletta). These nematodes can either be a self-fertilizing hermaphrodite where each animal produces both sperm and egg, or they can be males that will mate with the hermaphrodites (Brenner). When a hermaphrodite self-fertilizes, the chance of having a male being produced is extremely low at just 0.1%, however, when a hermaphrodite is fertilized by a male, the ratio of males to hermaphrodites is generally equal making it 1:1 (Altun).

The reason for doing this lab is to study evolution using small population of finches on two different islands. I used different charts and graphs to find limitations on natural selection. Then find the effect evolution will have on beak and population over a certain time period. Using three different assignments I have to develop different hypothesis comparing and contrasting the average beak size and population size changing rain fall, beak sizes, population and island size on Darwin’s and Wallace experiments.

Introduction: The biological membranes are composed of phospholipid bilayers, each phospholipid with hydrophilic heads and hydrophobic tails, and proteins. This arrangement of the proteins and lipids produces a selectively permeable membrane. Many kinds of molecules surround or are contained within

In the LULC/Soil/Slope tool box, the last option is slope tab. User has to select to number of slope classes for the watershed. Two options are available to define the slope discretization in the slope definition tool box. First one is single slope and second one is multiple slopes. If user select single slope in the tool box, it creates slope range class 0-999%, if user selects multiple slopes, the tool allows to create 5 classes. For the kaddam watershed five slope classes has been selected in the tool box. The classes are 1) 0-5% 2) 5-10% 3) 10-15% 4) 15-35% and 5) 35-9999%. The slope map of the kaddam shown in the

Cryptococcus neoformans (Cn) virulence depends on the active transport of vesicles that contain melanin and capsule precursors, proteinases, and other macromolecules. We previously found that the Cn intersectin protein Cin1 regulates intracellular trafficking critical for growth and virulence and that Cin1-S isoform confers a marked survival advantage in the CNS of a murine model of cryptococcosis. In addition, we found that the expression of extracellular RNAs (exRNAs) including small RNA (sRNA), mRNA, and long noncoding RNA (lncRNA) was significantly differentiated among cin1, CIN1-S, and wild type stains. Further investigation of these observations could promote our understanding of Cn propensity for the host CNS and the virulence

Observation: no bugs were found except small, black, gnats were all close to the ground.

The oxidation number of an atom of any free element is ZERO. Means to say there is only one kind of atom present, no charge.

Use a test tube holder to put the test tube into a container of boiling water for 5 minutes, or until the solution changes color.

Water uptake capacity of NCs enables them to entrap exudates upon contact with suppurating wounds which is desirable for their effectiveness as wound dressings. The increase in size and agglomeration of AgNPs from NC-1 to NC-3 might have resulted in more blockages of pores of CNCs which could be responsible for a decrease in water uptake capacity of NC-2 and NC-3 as compared to NC-1.

In Driscolls’s lab students were researching on the nervous system of the aging Caenorhabditis elegans, and also students were trying to sprout the neurite and deterioration of the synapse. C. elegans is a round worm which is an effective model for investigation of the rationed systems that adjust sound maturing. Students has reported that maturing C. elegans neurons can display novel neurite outgrowth from dendrites and from somata. New outgrowths can be exceedingly pervasive in maturing touch receptor neurons, with mitochondria regularly situated at branch locales. Diverse neurons display particular sorts of outgrowth, even with a solitary neuronal class. Be that as it may, not all neurons display morphological change with age, showing

C. elegans is an effective model for investigation of the rationed systems that adjust sound maturing. We report that maturing C. elegans neurons can display novel neurite outgrowth from dendrites and from somata. New outgrowths can be exceedingly pervasive in maturing touch receptor neurons, with mitochondria regularly situated at branch locales. Diverse neurons display particular sorts of outgrowth, even with a solitary neuronal class. Be that as it may, not all neurons display morphological change with age, showing cell-specificity of basic decrease. In the maturing nematode sensory system, neuronal passing or potentially distinguishable loss of procedures are not promptly evident, but rather in light of the fact that dendrite

It is of upmost important that immune defenses work in a regular coordinated manner so that the host can fight with infection and a regulated immune system prevent the deleterious effects of unchecked immune responses on host cells [63]. As Caenorhabditis elegans is susceptible to infection by a variety of fungal and bacterial pathogens so it employs a highly coordinated innate immune system to detect and counter pathogen attack, no matter the attacking pathogen is ingested or comes into external contact with the animal [64, 65].

The crude protein extraction of E. huxleyi cells that were either grown in 23℃ or 18℃, was prepared by grinding the cells in liquid nitrogen until it was fine powder. The protein extract was denatured and solubilized by adding 800 μL of an extraction buffer (30mM Tris-HCl, 7 M urea, 2 M thriourea, and 4% CHAPS), 8 μL of a protease inhibitor cocktail, 16 μL of B-mercaptoethanol, and shaking it for half an hour at room temperature. This process was done in order for no contamination to occur because, this algae’s electrophoretic separation of proteins concentrations is low.   Then the extract was transferred to a 1.5 ml microfuge tube and it was centrifuged for 30 minutes at 15,000 xg at 4 °C. The liquid portion of the extract was transferred

The purpose of this experiment is to test how location affects whether or not the clay caterpillar models will get pecked by birds. Twenty-one clay caterpillars were made and they were made to be 5 cm ± 0.01cm long and 1 cm ± 0.01cm in diameter. Seven caterpillar models were placed in each of the three locations. The three locations are my backyard, the park, and my community garden. As a control variable, all the caterpillars were placed 300 cm ± 50 cm above the ground. An equal amount of caterpillars were glued, to prevent them falling off, in similar areas such as trees, bushes, and fences. This experiment went on for three weeks. Each week is considered one trial. Each caterpillar that was pecked or not was recorded and the data was placed