C Elegans Research Proposal Paper

After gently blotting, weigh all three eggs separately inside their own individual weigh boats to the nearest 0.1g and record the weights in Table 1 at time 0. Place eggs 1, 2, 3 into separate culture dishes containing solutions of distilled water (0% sucrose), 14% sucrose, and 40% sucrose respectively. The sucrose must cover the entire egg inside the culture dish before proceeding to the next step. Place a napkin down on the lab counter, at 15-minute intervals, remove the eggs from the dishes with large spoon; place on napkin and wipe off all excess water each weigh boat must be clean as well before weighing; and again, weigh each egg separately. Record the weight in Table 1: Weight of Eggs vs. Time (minutes). Replace the egg in the dish for the next interval. After the experiment is complete, return the eggs to their container and each solution to its labeled container. Clean the dishes and weigh boats and return all materials to the setup

The content of this section depends to a large extend on the nature of the experiment. Topics here should include a section labelled:

Caenorhabditis elegans, a type of free-living nematode that is found in soil, is the subject matter for this experiment (Marsh). C. elegans allow for an exceptional model organism because it is easily raised in the laboratory setting, it reproduces rapidly, has a short life cycle of 3 days where it develops from egg to adult worm, only 1.3mm in length, and although only having 959 somatic cells, it shares many characteristics with other multicellular organisms like having organs and a nervous system (Kaletta). These nematodes can either be a self-fertilizing hermaphrodite where each animal produces both sperm and egg, or they can be males that will mate with the hermaphrodites (Brenner). When a hermaphrodite self-fertilizes, the chance of having a male being produced is extremely low at just 0.1%, however, when a hermaphrodite is fertilized by a male, the ratio of males to hermaphrodites is generally equal making it 1:1 (Altun).

2. This experiment is not able to support Matthew's hypothesis. Suggest specific improvements that will allow the experiment to more effectively test the given hypothesis. Explain why these changes are improvements.

(or Objective) This part of the experiment expressed clearly in only one or two sentences,

Write theories for each of your experiments performed here. They may not be the same as anyone else!

Chemical changes occurred in five out of the eight experiments completed in this lab. Although the main focus of experiment two was

To start out with a research where we use the experimental method, we first have to state what will be studied in our experiment. In this case, we will look at the problem of does sugar affect attention in small children. My hypothesis is that sugar will interfere with small children’s attention. The operational definition for affect attention will be measured by a learning lesson with the children. Where at the end of the lesson, children will complete a test from the subject that has been discussed during the lesson.

As researched, earthworms are known as hermaphrodites, which are defined as an animal having both male and female sex organs or other sexual characteristics, either abnormally or (in the case of some organisms) as the natural condition (Dictionary). During the earthworm lab, I discovered that earthworms have both seminal vesicles and seminal receptacles. Holding sperm after cross-fertilization has occurred is the seminal receptacles for females only. Seminal vesicles produce and release sperm and are well known as the male reproductive organs. Gizzards are responsible for mechanical digestion and grinding up the food for earthworms (Earthworm dissection video). On the other hand, for male crayfish to make sperm they must contain testes, unlike the females they

Based on the results of your experiment, would you reject or accept the hypothesis that you produced in question 1? Explain how you determined this.

- The use of a scientific control groups is important because it can segregate the effect of

Describe what you did in the experiment, and include methods and materials used. Do not

Dr. Bruce Wightman reeled in an overwhelming number of students, professors, and biology enthusiasts with his talk about his charming nematodes, Caenorhabditis elegans. Dr. Wightman’s experiment involved genetically analyzing a new sleep circuit in C. elegans. He initiated a discussion about a topic all college students can relate to: sleep. Animals (including humans) love to sleep, but why? Because it allows time for the brain to clear of any toxins, to produce new cells, and to consolidate new memories. Introducing his work, Dr. Wightman familiarized his audience about the glamorous and underappreciated C. elegans. The nematode, C. elegans, is the first multicellular organism to have its genome sequenced. This phenomenal organism has simply

the sperm are categorized as masculine and feminine. Finally, it seems that the end goal of

Many factors such as the egg’s structure and what the eggs are held in have to do with the