CHM2123 Lab 5

The objective of this lab was to create a ketone through an oxidation reaction using a using a secondary alcohol and oxidizing agent in order to use that ketone in a reduction reaction with a specific reducing agent to determine the affect of that reducing agent on the diastereoselectivity of the product. In the first part of this experiment, 4-tert-butylcyclohexanol was reacted with NaOCl, an oxidizing agent, and acetic acid to form 4-tert-butylcyclohexanone. In the second part of this experiment, 4-tert-butylcyclohexanone was reacted with a reducing agent, either NaBH4 in EtOH or Al(OiPr)3 in iPrOH, to form the product 4-tert-butylcyclohexanol. 1H NMR spectroscopy was used to determine the cis:trans ratio of the OH relative to the tert-butyl group in the product formed from the reduction reaction with each reducing agent. Thin-layer chromatography was used in both the oxidation and reduction steps to ensure that each reaction ran to completion.

27. Heat the water to 40°C with an alcohol lamp by setting up the apparatus as shown in figure 3 below:

This type of reaction is an oxidation-reduction (or redox) reaction. This reaction is also [anabolic/catabolic] and [endergonic/exergonic].

Abstract: Using hypochlorous acid to convert secondary alcohol called cyclododecanol to the corresponding ketone which is cyclododecanone by oxidation.

Oxidation-reduction reactions can be used to stereochemically control and produce many different organic molecules. The oxidation step in this process increases the number of carbon oxygen bonds by losing a hydrogen and breaking

The purpose of this lab report is to synthesize luminol and then test its chemiluminescent properties. 5-nitro-2,3-dihydrophtalazine-1,4-dione was reduced using sodium hydrosulfite in a solution of 3 M sodium hydroxide in water to form luminol. The product was then used to chemically generate light by reacting luminol with 3 M sodium hydroxide, hydrogen peroxide, and potassium ferricyanide. The reaction created a bright, blue light emission. From glow sticks to revealing remnants of blood samples in crime scene investigations, luminol, C8H7N3O2, has a wide variety of real world applications.

BI532: Skills for Bioscientists 2 Mini Project 2 report Effect on the velocity Alcohol Dehydrogenase on different alcohols under different conditions Abstract The effect on the velocity of Alcohol dehydrogenase producing an acetaldehyde was measured under different conditions to analyse the properties of the enzyme as well as the concentration the enzymes have the best rates. The velocity of alcohol dehydrogenase was measured against different substrates such as ethanol, propanol and propan-2-ol and using spectrophotometry at a wavelength of 340nm. While the enzyme velocity of ethanol production was high, propan-2-ol did not cause any changes the rate of acetaldehyde production. The amount of ethanol, which was the most reactive substrate, that reacted with the fastest rate was then calculated

The a-Ketoglutarate undergoes oxidative decarboxylation, loses a CO2 molecule and is then catalyzed by a-ketoglutarate dehydrogenase complex and becomes succinyl-CoA. In the process, a NAD+ molecules becomes an NADH molecule.

Nitric Acid was then added to the copper wire in order to make the copper wire dissolve. As soon as the acid was added, the beaker turned an orange color and the solution became green releasing gas into the air. This is defined as the dissolution of copper. This observation was expected to occur and happened as well. Cu(s)+HNO3(aq)→ CuHNO3(aq)

2. The type of insulation that we’ve chosen is the cotton, approximately with the same thickness for each flask. They will be changed for every trial, because for each trial, as the heat escapes from the sides of the flask in the form of water vapor, it comes in contact with the cotton, thus making it moist, and changes the capacity of the cotton to insulate. We have decided to

The lab performed required the use of quantitative and analytical analysis along with limiting reagent analysis. The reaction of Copper (II) Sulfate, CuSO4, mass of 7.0015g with 2.0095g Fe or iron powder produced a solid precipitate of copper while the solution remained the blue color. Through this the appropriate reaction had to be determined out of the two possibilities. Through the use of a vacuum filtration system the mass of Cu was found to be 2.1726g which meant that through limiting reagent analysis Fe was determined to be the limiting reagent and the chemical reaction was determined to be as following:-

b.) CH3COCH3and a reducing agent A ketone with a reducing agent will produce an alcohol. This is a redox reaction.

A hot plate was preheated to 100°C. A dry 5-mL long-neck round-bottom flask was clamped over an aluminum block placed on the hot plate. Ferrocene (0.09 g), acetic anhydride (0.35 mL), and 85% phosphoric acid was added to the flask in that order of addition. A magnetic stir bar was added to the flask. Solution was stirred and heated for 10 minutes. Flask was removed and allowed to cool to ambient temperature. DI water (0.5 mL) was added and the solution was cooled to 0°C by ice bath. The solution was neutralized with 3M sodium hydroxide dropwise while stirring and cooling. PH was monitor by pH indictor paper. Solid product was isolated by vacuum

The oxidation reaction occurs at the two alcohol groups attached to the bound hydroxytyrosol group converting them to keto groups as shown in Figure 2. The simple

Whereas, volumetric flask has TC as its marking, meaning it is marked as a ‘to contain’.