Caffeine And Daphnia Experiment

According to the results, the columns of caffeine in figure 1, of this experiment the hypothesis for caffeine is partially accepted. There is an increasing trend in the change of pulsation rates with increasing

At first the average Heart rate of Daphnia was 22 with no treatment after measuring it for three times. Afterward when I putted 15% of Ethanol on Daphnia I found out that Ethanol does affect Daphnia and caused to decrease the heart rate of Daphnia after measuring it for three times. Daphnia’s heart rate was decreased by 7 after putting Ethanol. I would classify Ethanol as a depressant because it decreased the heart rate of Daphnia.

This experiment is to see The Affect of Caffeine on Daphnia. Daphnia is also known as a water flea. It is a distant relative of crabs and shrimps. (Marshall Cavendish, 2002) Daphnia have a hard external skeleton with jointed appendages and limbs. The head contains a large central eye which is made of two eyes joined together. Daphnia has two pairs of antennae which are used for swimming. Each daphnia has five pairs of limbs. These limbs lie in a certain space under the body bounded by the carapace. (Marshall Cavendish, 2002) A carapace is a hard upper shell or chitinous outer covering on the back of some animals. The daphnia is well preserved because of its shells which are composed of chitin. The usual size of this microorganism is about 0.2-0.3 mm; they look like flat disks. Their bodies are divided into three parts – head, thorax, and abdomen. The head is typically dome-shaped with five pairs of appendages. Among these five are two pairs of antennae; there is a small pair and a larger pair. The smaller pair of antennae serves as a sensory function and the larger one is used for swimming. The other three appendages’ purpose is to secure food they intake. (Michael Hutchins, 2003) The thorax holds four to six pairs of legs that are used for gathering food, filtering water, or grasping mates. There are over 400 species of daphnia and they are distributed worldwide. Daphnia uses their thoracic legs to produce a constant current of water; this allows them to filter food

The projects purpose was to determine the effects of alcohol and caffeine on the heartbeat rate in Daphnia Magnus. Our hypothesis is alcohol causes a decrease in heart rate, whereas caffeine causes an accelerated heart rate, predicting that the more caffeine we give the daphnia the faster it heartbeat rate will become and the heartbeat rate will decrease as we give the Daphnia alcohol. After doing the experiment we found that the more caffeine we added to the Daphnia Magna the faster its heartbeat rate became. We also found that

The following was the procedure used by the team that introduced chemicals into the environment of the Daphnia. First a zero reading was taken before any chemicals were introduced. The zero reading was an observation of the Daphnia’s heart rate before any substances were administered. All fluids were drawn off the slide using the corner of a Kimwipe. Then two drops of two percent alcohol solution were dropped onto the Daphnia. After a minute a heart rate reading was taken. The same procedure, including using the Kimwipe to draw off previous solution, was then used with four, six, eight, and ten percent solutions. A heart rate reading was taken after each solution was introduced.

In Exercise 1, diaminofluorene is used to determine the hemoglobin concentration in the daphnids. A higher hemoglobin concentration is indicated by a darker blue color. A spectrophotometer was used to determine the absorbance at 610nm. When measuring the absorbance levels a blank is necessary to have a zero reference, the blank is the “starting point” for the measurements of the sample (re-word). The blank consists of 10µL of diaminofluorene(DAF), 50µL of hydrogen peroxide, and 0.5mL of PBS. The PBS acts as a buffer in this experiment. The cuvette with the sample of daphnids consisted of 10µL of DAF, 50µL of hydrogen peroxide, and 0.5mL of the sample of Daphnia. In Exercise 2, the Pasteur pipette was used to obtain the sample of Daphnia. The depression slide used in this experiment isolated the daphnid, cotton was used to keep the daphnid still while the heartbeat was counted. The ocular micrometer on the microscope allows the tail spine length to be measured accurately, as well as using an ocular magnification table.

This laboratory exercise was performed to visualize the effects of various drugs on the heart rate of Daphnia magna. The four drugs tested consisted of: Lidocaine, Acetylcholine, Caffeine, and Nicotine. These drugs were designed to have an apparent effect on the average heart rate of the Daphnia. The laboratory exercise was divided into two parts and procedures: measuring the basal heart rate of Daphnia, and measuring the drug induced heart rate of Daphnia. In order to measure the basal heart rate, various Daphnia were obtained and observed under a microscope at 40x magnification for two separate trials. Using the same technique, the drug induced heart rate with each drug (Lidocaine, Acetylcholine, Caffeine and Nicotine) was measured and

Thesis: Caffeine can have many different effects on the body depending on the amount of consumption.

The purpose of this experiment was to see if caffeine had an effect on the heart rate of Daphnia (water fleas) by observing them under the microscope and recording their heart rate over a period of time in concentrations of caffeine. Caffeine, which is a stimulant drug rttused in coffee, tea, Red Bull and Pro-plus, causes increased amounts of stimulatory neurotransmitters to be released. As caffeine is a stimulant, a prediction was made that caffeine would increase the heart rate of the Daphnia.

Throughout my experiment my control acted normally, the daphnia had average movement and calm manner. On a scale from 1 to 5 I ranked the control group as a 3 for normal movement and health. When I introduced car antifreeze to my pure water daphnia there was an immediate change in the color of the daphnia, their bodies turned from a white opaque color to a light pink (the same color as the antifreeze). Not only did the physical appearance change but their behavior became more aggressive and panicked. As time passed the daphnia moved in a circular motion and the chemical caused one of their antennas to cripple. The antifreeze surprisingly resulted in no deaths so I gave the effect of antifreeze to daphnia a 2 (table 2 and 2B). When exposed

In this experiment we find how caffeine can affect the heart rate of a culture Daphnia. Heart rate of a living organism’s can vary depending on the individual, age, body size, heart conditions, medication use and even temperature. This report will examine if the caffeine is good or bad for the living organism’s health and body. And discuss about where the caffeine is produced and used in daily life of human beings and on the environment. Daphnia is a water flea used in this experiment because of its genomic infrastructure with wide range of phenotypic diversity. This quality of Daphnia makes them a versatile model for the experiment. Also their transparent body allows the experimenter to visually see how the heart beats and count them under the light microscope during the experiment as required. The heart rate of Daphnia is monitored under different concentration of caffeine solution and the results are shown in a table and a graph. Experiment carried out to locate the effects of caffeine on a heart rate of Daphnia may or may not be a predictor of change in human heart rate under caffeine. The effects of caffeine can also be tested on humans but those experiment involving humans contains high risk, as Daphnia can only live for a short period of time and in nature most of them get eaten within their first few days or weeks of life.

This can also be related to Daphnia in the same way. Therefore I predict that as the concentration of alcohol surrounding the Daphnia increases the greater the percentage decrease of heartbeat. ABSTRACT -------- The aim of the investigation was to find out the effect of alcohol on the heart rate of Daphnia.

Dog owners should beware of caffeine poisoning as this concern has no antidote! Caffeine is a highly toxic for dogs and for other pets although this substance can be safely consumed by humans. Caffeine is a substance found in coffee, tea, chocolate, cocoa. The pleasant bitter taste in some soft drinks is due to the usage of caffeine as an ingredient. Dog owners have to make sure that the pet is prevented from consuming the above mentioned drinks and also from eating coffee grounds and coffee beans.

In the beginning of the semester we were told to write down any drugs we had taken in the last 30 days. When I wrote down the drugs that I had taken over the last 30 days I never once considered caffeine a drug. I never saw caffeine as a drug until this class.The purpose of this project is to get a better understanding of someone who is attempting the abstinence process. For this project we had to get rid of one substance we consume daily or at least frequently. When providing a list of three substances I consume daily or frequently I had not known what it was for. To my surprise the substance I would have to give up would be soda. Soda was the first substance on my list and was the one I would do the project. I wanted to challenge myself when it came to the project and not do the project on a substance I consume less frequently.

The primary objective of this research was to produce a study that displayed that chronic coffee consumption has preventative effects in mammals. This paper will allow me to trace the method that the scientists used in this particular mice experiment. Using the scientific method during the mice experiment allowed the scientists to ask certain questions, make observations, and receive answers to their questions. During this experiment twenty one mice were tested to determine whether water or diluted black coffee would lower the mice blood glucose levels. Through my research and examination of this experiment I will develop a hypothesis and come to a conclusion on the basis of the research provided.