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Candida Rugosa Experiment

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The experimental design of this research can be divided into five main stages. The first stage is development of yeast cell colony on nutrient agar and inoculum preparation of Candida rugosa ATCC 14830. The microorganism used in this research is isolated from natural soil. Second stage is extraction of lipase enzyme from the growth medium that contain olive oil that act as a substrate. Third stage of this project is preparation of immobilized and free enzyme culture system. The immobilized enzyme culture system is prepared with Ramsay medium as fermentation medium and calcium alginate gel beads as immobilized support. The next stage is data analysis on lipase activity on both culture systems. Finally the fifth stage is the comparison on efficiency between immobilized and free lipase culture system. The flow chart of experimental design is shown in Figure 4.1.

Isolation of yeast strain Candida rugosa from soil
Stock Culture
Streak plate and inoculum preparation
Inoculation of inoculum into growth medium containing olive oil and fermentation medium
Extraction of lipase enzyme into culture system containing calcium alginate gel beads Analysis :
• pH stability
• Thermal stability
• Reusability

Comparison on efficiency between immobilized and free enzyme culture systems Figure 4.1: Flow chart of experimental design The first stage is development of yeast cell colony on nutrient agar and inoculum preparation of Candida rugosa. Sample was collected locally in
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