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Capturing Brycon Henni Individuals

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Material and Methods
SAMPLE COLLECTION: Brycon henni individuals were captured using cast nets, backpack electrofisher SAMUS 725MP, electrofishing, hooks and nets in the middle basing of the Nare and Guatapé rives, in the river chanel and associated creks (Fig. 1, Table I), Antioquia, Colombia. Fish were captured with between November of 2007 and March 2010. Labeled individuals were preserved in a 10% formalin solution and carry out to the laboratory. Subsequently, formaldehyde was washed three times with water and changed by 70% alcohol for preservation and storage.
MORPHOMETIC VARIABILITY: Sex identification was carried out through direct observation of the gonads based on the characteristics described by (Holden & Raitt 1975) individuals with a Standard Length (SL) equal to or greater 119.7 mm for females and 82.7 mm for males was considered as adults (Mancera-Rodríguez et al. n.d.).
Digital images were captured for 156 individual corresponding to 78 males and 78 females, that did not alter its form during the process of preservation. The individuals were located laterally in the center of the visual field with graph paper in the background at 335mm of focus distance using a Canon PowerShot A430 digital camera mounted on a tripod. We used the module COO V-41 of the CLIC 45 software (Dujardin 2012) to digitize the 13 landmarks: (1) snout, (2) supraoccipital, (3, 4) dorsal fin, (5) Start adipose fin, (6, 7) caudal peduncle, (8, 9) anal fin, (10) Start pelvic fin, (11) Start

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