Case Study 2 WwWL

The carrier then returns to its original shape, releasing the two K+ions and the remnant of the ATP molecule to the inside of the cell. The carrier is now ready for another pumping cycle” (McCance & Huether pg. 29)

3. What would happen to the amount of H+ secreted into the renal tubule if the activity of the Na+ /K+

In photosynthesis H+ ions are vital in the production of the energy source that is ATP, which is used in several metabolic processes, such as respiration. The photolysis of water produces H+ ions, electrons and O2. The excited electrons lose energy as they move along the electron transport chain, this energy is used to transport the H+ ions (protons) in to the thylakoid, which causes a higher concentration of H+ than there is in the stroma, thus causing a proton gradient across the membrane. The H+ then proceed to move down the concentration gradient into the stroma via the enzyme ATP synthase. The energy from this process is called chemiosmosis and combines ADP with inorganic phosphate (Pi) to form ATP. Light energy is then absorbed by photosystem I (PS I) which excites the electrons to a higher energy level. These electrons are transferred to NADP with H+ ions from the stroma to form reduced NADP. The whole of this process is

This energy is used to re-form the bonds between ADP and P to make ATP.

9. The movement of electrons form the proton gradient, the electron transport chain pumps Hydrogen plus from the matrix to the intermembrane space. This causes more protons outside than inside. The first and third complexes of the electron transport chain move the protons through the membrane. The first complex in the electron transport chain pumps

Cellular respiration is creating ATP from ADP and a phosphate inorganic using the energy which was released from breaking apart glucose. The equation that summarizes this process is (ADP + Pi) + C6H12O6 +6O2 → 6H2O + 6CO2 + heat + (ATP). ATP is made up of a sugar ribose, 3 phosphate groups, and adenine. ATP is the energy used to complete processes in the body. ATP also has a very high potential energy because of its phosphate groups. Potential energy has to do with energy due to location. For example, a person on a diving board has a higher potential energy than a person already in the water. This is because the girl on the diving board has more potential to fall or convert the potential energy into kinetic energy by using her location to power her fall. The ATP has higher potential energy because its phosphate groups have oxygen ions. The negatively charged oxygen ions repel each other and do not want to be near to one another. Because of this, if the third phosphate group was to break off of the ATP molecule, an amount of energy would be released, lowering the potential energy. This is why ATP has such a high energy and is used for so many processes. The ATP would become ADP with a phosphate group becoming inorganic and would release energy.

___The concentration of extracellular K+ ions would be high and this would prevent more K+ ions from diffusing out of the cell.

3. Describe what would happen to the resting membrane potential if the sodium-potassium transport pump was blocked.

The third and final step in cellular respiration is the electron transport chain which takes place in the inner mitochondrion membrane. This process uses the high-energy electrons from the Krebs cycle to convert ADP into ATP. These high-energy electrons are first passed along the electron transport chain. Every time 2 electrons travel down this chain, their energy is used to transport hydrogen ions (H+) across the membrane. These H+ ions escape through channels into an ATP synthase. This causes it to spin, transforming the ADP into ATP. On average, each pair of high-energy electrons that moves down the electron

With an important level of lactate and a prosperous protons gradient, we can see a

As it travels, it generates energy that makes ATP, and sends protons across the membrane

This results in increased energy expenditure, fuel mobilization and oxidation for energy extraction, oxygen consumption, respiratory rate, and heat production and release (Dauncey.,1990). The stimulation of the respiratory rate would intuitively lead to greater ROS production but, the relation between these two variables is not linear. Instead, ROS production depends largely on the mitochondria. Mitochondria are the primary intracellular site of oxygen consumption and the major source of reactive oxygen species (ROS), most of them originating from the mitochondrial respiratory chain(Armstong &Jones.,2002). Although THs do not directly determine the respiratory state of the mitochondria (Katyare and Raian.,2005), stimulation by THs by augmenting ATP breakdown by different energy-consuming mechanisms in the cell (Dauncey.,1990) and thus increasing ADP availability. This would be expected to decrease ROS production. However, THs also promote a reduction state in the cell by increasing fuel availability and extramitochondrial production of ATP and NADH, which in turn promote reduction of the components of the mitochondrial respiratory chain

Consequently, a hydrogen ion force is generated by an electron transport chain, which acts as a pump, and it pumps hydrogen ions throughout the membrane (Wikipedia, n.d.) The electrical charge is separated, and in the mitochondria, energy is released by the electron transport chain. This energy is used to move hydrogen ions from the mitochondrial matrix to the stroma. Therefore, hydrogen ions change inside the membrane. They move from a higher concentration to a lower concentration. Moreover, glucose is completely broken down and eventually stored for

Mitochondrial respiration (aerobic metabolism) occurs in the mitochondria of the cells when the oxygen supply is sufficient to meet the oxygen demands (Smith, 2002).

In the metabolic reactions, oxidation-reduction reactions are very essential for ATP synthesis. The electrons removed in the oxidation are transferred to two major electron carrier enzymes. The electrons are transported through protein complexes in present in the inner mitochondrial membrane. The complexes contain attached chemical groups which are capable of accepting or donating one or more electrons. The protein complexes are known as the electron transfer system (ETS). The ETS allow distribution of the free energy between reduced coenzymes and the O2. The ETS is associated with proton (H+) pumping from the mitochondrial matrix to intermembrane space of the mitochondria.