Case Study: Mega Base Pair Mycoplasma Mycoses

The vital components and techniques of gene cloning are as follows, the DNA sequence that contains the desired gene (EZH2) is amplified by Polymerase chain reaction. PCR was established by Kary Mullis in 1985, popularly known to amplify target sequences of DNA (EZH2) to a billion fold in several hours using thermophilic polymerases (Taq) ,primers and other cofactors (Sambrook and Russell, 2001). Three crucial steps are involved which are Denaturation (at 95°), Annealing of the forward and reverse primers (55-65°) and lastly primer extension (at 72°). After amplification the desired sequence is integrated into the circular vector (pbluescript) forming the recombinant molecule. For the compatibility of the insert and vector, both were digested with (EcoR1) so the same cohesive ends are generated in both, making it easier to ligate. EcoR1 is a restriction enzyme that belongs to the type II endonuclease class which cuts within dsDNA at its recognition site “GAATTC” (Clark 2010; Sambrook and Russell, 2001).

Throughout our lifetime we are in constant change, many of those changes are ones that you don’t notice. However permanent we set a change to ourselves, one thing we hardly notice change is our DNA. Just growing recently in popularity is genetic engineering , along with its immense potential which can do very much for us, in the future and today. However, with all the potential that genetic engineering carries many people have begun to resent what genetic engineering could become instead of focusing on the great capabilities, and what lies ahead of its bright future. Regardless of what others believe, Genetic engineering will renovate the way we do things today, genetic engineering is a tremendous step to accept, and without a doubt a step

The altering of human genes could save lives. You could cure cystic fibrosis or alzheimer's. This would save the lives of many (Doc. 3). This technology could also give you children with specific traits of your choice. Also, this engineering can leave people painfree. This is not good because they can’t detect danger. As a plus side, scientists will eventually take the gene that causes this and help cure those with chronic long lasting pains (Doc. 2). This would make more people happy and healthy across the nation! Eventually we could go so far as to make a genetically engineered nation. As you can see, Genetic Engineering also could have a positive effect on

The progression of cloning technology could prove to be of great use to humans in the future. Further experiments on cloning will add to the understanding of genetics and lead to the production of animals organs that can be accepted by humans. Theoretically cloning could eliminate all problems regard organ transplants by producing animals that can act as suitable organ donors. More extensive cloning processes could also provide a solution for the world’s food inequity problems. Stronger, more resistant plants could be cultivated in large amounts through means provided by cloning techniques. The same idea could be applied to livestock, where common diseases can be eradicated to create stronger breeds.

Genetic Engineering has produced an array of breakthroughs in the progression of modern medicine. Though it is faced with both ethical and potential environmental grievances, the efficacious results of Genetic Engineering counterpoise the criticisms from environmentalists. Researchers in genetics have provided simple solutions to problems that society has consistently endured. Genetic Engineering offers solutions to failing crops and heightened yields in production as well as numerous applications in medicine. It is approached by the concerns of the environmental impact of such actions, as well as the civil concern on ethics.

Human genetic engineering can save people's lives now and could save more in the future. According to a New Scientist article (Le Page, 2015), Layla was three months when she was diagnosed with acute lymphoblastic leukaemia, a kind of cancer in the bone marrow. She underwent other treatments like chemotherapy, but those failed so she underwent gene therapy by using the TALEN protein to edit her genes to produce a cancer fighting T cell called UCRAT19; She survived and thrived (Le Page 2015). Saving someone’s life through human genetic engineering doesn’t even have to be as complicated as altering genes. According to TIME Health (Park, 2014), Mya Burkhart was six months old when her parents started to look for a cure to her rare mutation in the gene for citrate. This mutation prevented certain parts of her from getting enough energy, causing breathing and development problems, and after having no luck with any other diagnosis, a genetic scan figured out what the issue was and gave her citrate supplements (Alice Park, 2014). Both Layla’s and Mya’s lives were saved because of human genetic engineering. Layla was cured of cancer because of human genetic engineering, which is one toughest diseases for humans to cure right now, while Layla’s cancer was cured, but Mya’s condition was only treated so maybe if human genetic engineering could continue a cure could be developed to improve her life.

The Gene Hackers published by the New Yorker’s Michael Specter talks about a breakthrough technology that could allow for the creation of genetically modified humans- “humans 2.0” as he calls it. This new technology could also be the cure for many genetic disorders like Alzheimer’s, Huntington’s etc. as it would allow scientist the ease of genetically identifying and editing specific genes responsible for these genetic abnormalities. This exciting new technology will be known as Clustered Regularly Interspaced Short Palindromic Repeats associated protein 9 or CRISPR-Cas 9 for short.

ROCKVILLE, MD and San Diego, CA (May 20, 2010)— Researchers at the J. Craig Venter Institute (JCVI), a not-for-profit genomic research organization, published results today describing the successful construction of the first self-replicating, synthetic bacterial cell. The team synthesized the 1.08 million base pair chromosome of a modified Mycoplasma mycoides genome. The synthetic cell is called Mycoplasma mycoides JCVI-syn1.0 and is the proof of principle that genomes can be designed in the computer, chemically made in the laboratory and transplanted into a recipient cell to produce a new self-replicating cell controlled only by the synthetic genome.

The biotechnology revolution will change the face of the planet, Introduction page 1, There are two thousand laboratories in america alone five hundred companies spending five billion dollars on the biotechnology. Among the scientist there are no surveillance to make sure they are doing the right thing with the technology they are creating. Page 2 of intro, International Genetic technologies, inc did secret research when an accident occured twelve people or fewer where there to witness the accident and only a handful survived. “Biotechnology will transform every part of human life from our food to our medical care our entertainment and our very bodies.” Biotechnology will be something that will either kill us or help us how we use it will depend.

Imagine the possibility of eliminating serious genetic diseases from the world. Imagine the idea of treating, preventing or even curing diseases that are yet to be cured. Imagine the feeling of being given improved health and a prolonged lifespan. This can all be accomplished with the aide of genetic engineering. Human genetic engineering refers to the process of directly manipulating human DNA to produce wanted results. DNA is a simple but very complex chemical that has the power to change the world and has begun to do so already. Many opponents to gene therapy fail to realize that genetic engineering has great potential to become very important in the biomedical industry. Though controversy exists regarding the ethics of human genetic engineering, it can produce numerous benefits, which outweigh its disadvantages and side effects; therefore, scientists should be able to manipulate the human genome for the purpose of helping people with serious medical conditions.

A basic method in which we get specific genes integrated with another organism’s chromosome is as follows: Isolate the DNA from which selected gene is to be taken from and treat it with enzymes that will cut out that specific gene. These genes are then inserted into bacteria and grown into colonies being

There are many risks involved in genetic engineering. The release of genetically altered organisms in the environment can increase human suffering, decrease animal welfare, and lead to ecological disasters. The containment of biotechnological material in laboratories and industrial plants contributes to the risk of accidental release, especially if the handling and storage are inadequate. The purely political dangers include intensified economic inequality, the possibility of large-scale eugenic programs, and totalitarian control over human lives. How should the acceptability of these risks be determined? We argue that the assessment should be left to those who can be harmed by the decisions in question. Economic

Genetic engineering has become increasingly normalized in today’s society, and people are exposed to this technology now more than ever before. Most people are aware that food companies practice genetic engineering on their plants in order to design the most profitable crops, but it isn’t generally known that this same technology can be applied to humans. The concept of picking certain traits and characteristics of a human may appear desirable, but many risks and potential side effects may follow considering that it is unknown what genetic engineering could affect in future generations. Francis Fukuyama, an accomplished and distinguished professor of political economy and philosopher, conveys his concern that genetic engineering is developing at a surprisingly rapid rate. Within his book, Our Posthuman Future: Consequences of the Biotechnology Revolution, he claims that genetic engineering not only will potentially be detrimental for the human race, but due to the change in nature of human beings, such engineering will also result in significantly impacting government and politics. Although genetic engineering can be seen as a huge technological advancement that could potentially help millions, there are drastic negative effects and reasons for disapproving genetic engineering that are too important to be overlooked.

We are consuming genetically modified and processed foods every day. Genetically modified and process foods cover roughly 60 to 70% of our food supply. Supermarket processed food items now "test positive" for the presence of genetically modified ingredients. The "hidden menu" of these unlabeled genetically modified foods and food ingredients in the US now includes soybeans, soy oil, corn, potatoes, squash, canola oil, cotton seed oil, papaya, tomatoes, and dairy products. But genetic manipulation is something so very different. No one has seen a "gene", and the best teaching by the brightest minds cannot adequately convey the ideas of the uniqueness of the genome and how we can read this uniqueness. In short, very few people understand the

With only a 20-year history, genetic engineering is only a young science with much uncertainly relativity compared to other fields. Much of the elements bioengineering studies and experiments, the DNA genome and sequence, are still unknown to the scientific community. Changes in genes greatly transform the condition, structure, and essence of an organism, giving us an entirely different and unnatural creation. Even when the physical effects are barely noticeable or deemed safe and ethical, these modified creations’ effects on the environment are impossible to predict and will only emerge when the damage done becomes evident to the human population. Though a large unknown looms over modifying genes, bioengineering industries insist upon dismissing the scare over genetic foods with the vast potential “miracle” foods can benefit upon human society.