Case Study Of Escherichia Coli

The dirtiest thing you own maybe your cell phone. Imagine what touches your phone the most, probably your hands and face. Since cell phones are everywhere and have become one of the most helpful tools of our generation, they have their drawbacks. The use of cell phones happens everywhere today, one place being the hospital. From a study of phones and spreading of bacteria associated with health care associated infections, Karabay states, “Our study reveals that mobile phones may get contaminated by bacteria (such as Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae), which cause hospital infections, and may serve as a vehicle for the spread of nosocomial pathogens” (Karabay, 2007). Touching contaminated

A sample of the patients bacteria causing the urinary tract infection can only be used if a urine sample is bacteriologically viable (1 Radar). Infected urine and sterile urine are common samples used for the diagnosis of patients (1 Radar). It is important to gain further knowledge of multiple organisms’ resistance to antibiotics that cause urinary tract infections (1Radar). Escherichia coli has distinct characteristics and structures that help identify their unique properties (2 Johnson). Escherichia coli is the most common and most prevalent bacteria present in the fecal flora of the human colon (2 Johnson). Because of Escherichia coli unique ability to overcome the obstacles of a new environment, it makes easy for bacteria to spread into the urinary tract and produce a discomforting infection (2 Johnson). All research conducted lead to the belief that Escherichia coli was the Enterobacteriaceae pathogen causing the UTI in the patient. In order to confirm this prediction a set of differential tests and biochemical test must be conducted.

E. coli O157 infection can lead to hemolytic uremic syndrome (HUS), characterized by hemolytic anemia, thrombocytopenia, and renal injury. Still, it was not until 1993, after a large multistate E. coli

coli were labeled as bacteria that should not be examined or touch without permission from the supervising teacher. Although the strain of E. coli used in this experiment was harmless, to avoid transmitting the bacteria to other organism, the experimenter handled the E. coli for caution (see Appendix H, I, J, K).

2. List what it is caused by (specific scientific and common names of the organism: Escherichia coli

The priority nursing diagnosis of hospital acquired infection is risk for any kind of infection. One of the main goals for each patient in the hospital is the patient will remain free of infection as evidence by absence of heat, pain, redness, or swelling in any area of the patient’s body during each nurse’s shift. (care plan book). Frequently hand washing is the best intervention for preventing infection. Hand washing reduces the risk of transmission of pathogens by inhibiting the growth of or killing the microorganisms. (cb)Proper sterile technique during urinary

The role of the Registered Nurse is crucial in the prevention of hospital-acquired urinary tract infections. Of all the UTIs acquired in the hospital, 80% of them can be attributed to the use of

Hospital-Acquired Infections entail, urinary tract infections, pneumonia and bloodstream infections. In addition to the patients, the Infections tend also to affect the healthcare workers who usually take care of the patients who are the main sources of the of the infectious diseases in the hospitals. The project tends to investigate the best evidence-based practices and approaches used to reduce the rate transmission and spread of the HAIs, such as washing of hands by healthcare workers. The statement of the problem focuses on the best methods and efforts that can be developed and implemented in the hospitals with the objective of preventing the Hospital Acquired Infections contributed by healthcare workers and the patients who come for treatments at the hospitals (CDC, 2016).

Most of the germs causing bacteria are transmitted to the patient by hands during the patient care (World Health Organization, 2016). The surfaces in patient rooms, the health care workers (HCWs) clothing, and the instruments used by the HCW can be contaminated. Proper hand hygiene is needed to prevent or reduce the transmission of HCAIs in patients. Urinary tract infection, surgical site infection, pneumonia and infections of the bloodstream can be contacted through improper hand hygiene. HCAIs are the major cause of morbidity and mortality (Office of Disease Prevention and Health Promotion (ODPHP), 2016). These infections cost the health care system billions of dollars every year and several losses of lives. It can have disastrous emotional, financial and medical impact on patients and their family members. These infections jeopardize the safety and care for patients (WHO, 2016). Hand hygiene is the first line of defense against infections. Before contact with patient, hand hygiene should be performed by hand washing with soap and water or using an alcohol-based hand rub. Hands should be disinfected before patient contact, prior to and following the insertion of any invasive devices, before using gloves for central intravascular catheter insertion (Centers for Disease Control and Prevention (2016). Hand should be cleaned following checking vital signs, contact with mucous

Figure xxx: The total plate count of the Escherichia coli from day one to seven.

The purpose of this experiment is to show how different concentrations of Ampicillin affect Escherichia Coli Growth and how the bacteria become resistant to the antibiotic drug. Through a series of steps, which involves streaking agar plates with E.coli sample and application of ampicillin to the E.coli sample on the agar plate, the experiment yields a result that supports the hypothesis. The hypothesis acclaims that ampicillin would affect the growth of E.coli; measuring the zone of inhibition approves the claim in the experiment. The measurements of the zone of inhibition indicate that the generation of E.coli expands as the radius reduces. The reduction of the radius shows the E.coli population is reducing and becoming resistant. In other

The purpose of this lab experiment was to measure the population growth of Escherichia coli, or E. coli, a type of bacteria that can be found in the human body, specifically in the large intestine. It works to aid in digestion as well as the absorption of essential nutrients. While E. coli is extremely beneficial to the human body and most populations are safe for humans, some cause disease if they end up in food that is eaten (Trzepacz et al.) Sickness can occur when waste reaches a water supply, eating meat that is not properly cooked, or drinking milk that is not pasteurized properly. When working with this form of bacteria, it is extremely important to keep it contained for safe measure (Belk et al.) Additionally, this bacterium has also been cited as a cause of infections involving the bloodstream that have life threatening consequences. It is increasingly troubling because antibiotics are now becoming resistant (Collington, 2009.) E. coli is like all other bacteria in the way it carries out reproduction, in that it produces asexually through the process of binary fission.

In a pre-test, post-test research study conducted by Tillekerante et al. (2015), a multifaceted intervention targeting health care personnel was implemented to reduce CA-UTI incidence at a 169-bed, low economic hospital in Nairobi, Kenya. Surveillance activities were conducted in the 4 medical wards of the hospital, which contain a total of 48 male beds and 48 female beds. Active surveillance was carried out on the medial wards to determine the base line rate of symptomatic CAUTIs. Surveillance was conducted over an 8-week period form March 2012 to May 2012. It was carried out by members of the study team including 1 infectious disease physician and 2 local, bilingual research assistants trained in surveillance activities. The total number

Lehne (2013) continues by stating that in nosocomial caused cystitis E. coli makes up only 50 percent of UTI’s. In hospital settings more common gram negative causative agents of cystitis are Pseudomonas, Proteus or Klebsiella pneumoniae. There is also a gram positive agent named Staphylococcus saprophyticus that makes up 10 to 15 percent of nosocomial UTI’s.

Following contact with patient and or contaminated environment, germs can survive on hands for differing length of time(2-60mints).in absence of hand hygiene action the longer the duration of care, the higher the degree of hand