It was hypothesised that, if the Catalase is heated up to 37°C then it will produce oxygen faster that when it is at 23°C or 5°C because 37°C is the optimal temperature for Catalase. This was hypothesized because Catalase is a type of protein and when exposed to unfavorable temperatures, Proteins slow down or denature.
It was hypothesised that, if the Catalase is heated up to 37°C then it will produce Oxygen faster that when it is at 23°C or 5°C because 37°C is the optimal temperature for Catalase. The hypothesis is supported by the data because the overall rate of reaction for 37°C is 3.0%, for 23°C its 2.4%, and for 5°C it is 0.7%. The rate of reaction shows how much Oxygen is produced in a minute when the Hydrogen Peroxide is broken down
In this laboratory exercise, studies of enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes.
The purpose of this experiment was to simply measure oxygen production rates released from decomposed hydrogen peroxide under different conditions (concentration of enzymes, temperature, and PH level).
Students will be observing normal catalase reaction, the effect of temperature on enzyme activity, and the effect of pH on enzyme activity in this experiment. The enzymes will all around perform better when exposed in room temperature than when it is exposed to hot and cold temperatures. This is based on the fact that the higher the temperature, the better the enzymes will perform, but as the temperature reaches a certain high degree, the enzymes will start to denature, or lose their function.
Part A was just a beginning. The experiment showed us what a normal catalase reaction really is. We had one basic procedure; observe what happens when you add hydrogen peroxide to beef liver. At first we were just amazed by the bubbling product, we did not realize that a chemical reaction was going on. We had to redo the experiment so we could note that heat was being released as well as a combination of harmless gasses. We timed the reaction and learned to estimate the reaction rates by the time. We descovered that the bubbles were actually two main gasses, 2H2O and O2. They were being released due to the break down of a harmful gas H2O2. Another experiment we did was testing if catalase was reusable. We discovered that it was, in fact, reusable. The basics of a normal catalase reaction is what we learned about part A. Everything kept growing more and more into depth with our experiments as we went on during our lab.
As seen on table 1, the hypothesis in the introduction of this lab has been supported by the procedures. As the temperature varied from catalases optimal of 37° C, the reaction rate of catalase decreased. 37°C had the highest reaction rate of the three, at 3, while 4°C had the middle rate of reaction at 2.5, and 100° C had the lowest reaction rate of 0.5.
Purpose: The purpose of this lab is to test the enzyme catalase to see if adding more enzymes speeds up the reaction and, we will test it with pH and different temperatures. Also, I will be using potato juice as a catalase and measuring the amount of bubbles you get when you mix it with hydrogen peroxide. Procedure: Experiment 1: Enzyme- Substrate Specificity Obtain 3 test tubes and fill them up to 1 cm with potato juice and 5 cm with the hydrogen peroxide.
Discussion: The purpose of this experiment was to observe what the normal enzyme1 (proteins that catalyze chemical reactions) activity of beef liver catalase was, and to measure the effects of extreme changes in temperature and pH on the beef liver catalase function. We also observed what the bromelain1 (proteins in pineapple) activity was in a fresh pineapple compared to canned pineapple. Enzymes are present in all living things.1 These specialized proteins speed up chemical reactions fast enough to sustain life.1 This lab was driven by the question; if there is an extreme change in temperature or pH on an enzyme catalase, or a bromelain enzyme, then that catalase or bromelain will be affected or denatured.
reaction rate increases. If the temperature of an enzyme gets to high the reaction rate will slow
In temperature, when it increases, it is expected that the activity of Catalase will also increase up to a certain temperature. After a certain degree, it is expected that the activity of Catalase will slow down and eventually come to stop. Similarly, it is expected that more concentration of substrates will lead to higher activity of Catalase up to a certain concentration level. Past that certain level, the productivity of the enzyme will stop. On the other hand, for pH and salt concentration level, it is expected that too low or too high in either variable will lead to
Within a cell, enzymes are used as a catalyst to increase the rate of chemical reaction. They do not consume themselves, rather they help in increasing the rate of reaction. Within the body, enzymes vary depending on their specific functions. For instance, hydrogen peroxide is a toxic chemical, but it breaks down into harmless oxygen and water. This reaction can be sped up using the enzyme catalyst produced by yeast. Hydrogen peroxide is produced as a byproduct in cellular reaction, because it is poisonous and must be broken down, therefore this reaction is important. The speeding up of the reaction is shown below:
The biggest problem my group experienced was that as we moved into our second round of trails, we noticed consistently higher reaction times for the same concentration of enzyme. There are two possible explanations for this. The first is that we continued to use the same container of hydrogen peroxide every trail. The peroxide may have become diluted, reducing the rate at which the reaction could happen. This became apparent when we got to the lowest concentration trails. We were reasonably sure that these would both take a long time, so we did both trails simultaneously in two different containers: one old, one new. The trail conducted in the container of fresh peroxide finished in just over six minutes, however the trail conducted in the used
Testing the Effects of Temperature on the Decomposition of Hydrogen Peroxide with the Enzyme Catalase
The purpose of this experiment was to record catalase enzyme activity with different temperatures and substrate concentrations. It was hypothesized that, until all active sites were bound, as the substrate concentration increased, the reaction rate would increase. The first experiment consisted of five different substrate concentrations, 0.8%, 0.4%, 0.2%, 0.1%, and 0% H2O2. The second experiment was completed using 0.8% substrate concentration and four different temperatures of enzymes ranging from cold to boiled. It was hypothesized that as the temperature increased, the reaction rate would increase. This would occur until the enzyme was denatured. The results from the two experiments show that the more substrate concentration,
Biology 121 KS Lab Report IV 03/06/2018 Jannet Marin Testing the Effects of Temperature and pH on Potato Catalase Activity Introduction: To bear life in the cell, nearly all metabolic processes need enzymes. Enzymes are “macromolecular biological catalysts that help to speed up, or catalyze, chemical reactions.” The activity of enzymes is also affected by changes in the pH and temperatures. However, tremendously high or low pH and temperature values could cause complete loss of activity for most enzymes. Catalase is the most common enzyme found in potatoes and almost all organisms that are exposed to oxygen.
Observation: The enzyme catalase naturally existing in yeast reacts with H2O2 to create oxygen and water, causing a yeast soaked paper to float after a certain amount of time.