Catalase Lab Report

We set up 3 fermentation set-ups, labeling them 1, 2, and 3. Then, filled a tub with hot water and inserted the end of the plastic tubing into one of the test tubes and submerged the collection tube and plastic tubing in the tub. After that, we mixed the fermentation solutions for the other tubes, (tube 1 got 4mL of water and 3mL of corn syrup, tube 2 got 3 mL of water, 1 mL of yeast and 3 mL corn syrup, tube 3 got 1 mL water, 3 mL yeast and 3 mL of corn syrup) . We then mixed each test tube and put the rubber stoppers in the fermentation tubes. Finally, we marked the water level on each collection tube with a wax pencil to use as the baseline. Then at 5 minute intervals we measured the distance from the baseline for 20 minutes.

Students will be observing normal catalase reaction, the effect of temperature on enzyme activity, and the effect of pH on enzyme activity in this experiment. The enzymes will all around perform better when exposed in room temperature than when it is exposed to hot and cold temperatures. This is based on the fact that the higher the temperature, the better the enzymes will perform, but as the temperature reaches a certain high degree, the enzymes will start to denature, or lose their function.

Enzymes are biological catalysts that speed up chemical reactions, without being used up or changed. Catalase is a globular protein molecule that is found in all living cells. A globular protein is a protein with its molecules curled up into a 'ball' shape. All enzymes have an active site. This is where another molecule(s) can bind with the enzyme. This molecule is known as the substrate. When the substrate binds with the enzyme, a product is produced. Enzymes are specific to their substrate, because the shape of their active site will only fit the shape of their substrate. It is said that the substrate is complimentary to their substrate.

12.Stir then pour 2.5ml of the enzyme mixture into one of the test tubes not allowing any water from the saucepan into the test tube.

Enzymes are biological catalysts, which speed up the rate of reaction without being used up during the reaction, which take place in living organisms. They do this by lowering the activation energy. The activation energy is the energy needed to start the reaction.

The control experiment for this investigation will be the experimental setup of 5 trials using 5oC as the temperature. All the steps in the method will be followed.

The first experiment begun by filling a 600-ml beaker, almost to the top, with water. Next, a 10-ml graduated cylinder was filled to the top with water. Once water was added to the beaker and graduated cylinder, a thumb was placed over the top of the graduated cylinder. This would ensure that no water was let out and no bubbles were let into the graduated cylinder. Next, it was turned upside down and fully submerged into the beaker. Then, a U-shaped glass tube was attained. The short end of the glass tube was placed into the beaker with the tip inside of the graduated cylinder. Next, a 50-ml Erlenmeyer flask was received. After, 10-ml of substrate concentration and 10-ml of catalase/buffer solution were placed into the flask. A rubber stopper was then placed on the opening of the flask. After adding these, the flask was held at the neck and spun softly

4.Measure 35mL of warm water and add them into each of the 4 test tubes at about roughly the same time. It is essential that the water is warm. Do not seal the test tube.

1 ml of water should be added to the first test tube and make a note. In the second test tube, 1 ml of methyl alcohol should be added. In the third test tube, 1 ml of hexane must be added. Lastly, the fourth test tube will be a control.

It catalyses the hydrogen peroxide to oxygen and water, such as this formula: 2H2O2→O2+2H2O However, the effects of catalase is influenced by the substrate concentration. The different levels of the concentration will lead to different results of oxygen produced off the Hydrogen peroxide, Increasing the substrate concentration will increase the amount of the oxygen produced. Investigation Question: How Does increasing the concentration of Hydrogen Peroxide affect the amount of oxygen formed?

The enzyme is found in high gear assiduity in a organelle in cellular telephone called the peroxisome. One of the functions of catalase is to prevent a toxic accumulation of hydrogen hydrogen peroxide (H2O2) in cells. It catalyses the spiritual rebirth of hydrogen peroxide to water and molecular oxygen. Hydrogen peroxide is a by ware of metabolic cognitive operation . It is usually produced in peroxisomes when they partially oxidize roly-poly acids. When catalase is absent, the reaction it catalyzes is spontaneous, but at very low rates that are not able to reduce the harmful effects of hydrogen peroxide

After the data was collected from all 27 experiments of cold, room temperature and warm potatoes it was placed into excel to find the average rate of oxygen production, the average total oxygen after the timed period and use the information to graph the data.

Catalase is an enzyme that is present in most cells and is present in the liver. An enzyme is made out of proteins, there is a primary structure which is the sequence of amino and then as these twist and turn they become functional. Enzyme are very specific. When catalase is present in the liver it allows the hydrogen peroxide to be broken down into water and oxygen gas is released. The group was responsible to test a solid piece of liver at room temperature, which was the control, and frozen liver, which was the experimental component in the lab. The purpose of this experiment is to study and test the effects of an enzyme on a chemical reaction. This chemical reaction allows hydrogen peroxide to be broken down into water and oxygen and this

This experiment is designed to analyze how the enzyme catalase activity is affected by the pH levels. The experiment has also been designed to outline all of the directions and the ways by which the observation can be made clearly and accurately. Yeast, will be used as the enzyme and hydrogen peroxide will be used as a substrate. This experiment will be used to determine the effects of the concentration of the hydrogen peroxide versus the rate of reaction of the enzyme catalase.

Collect to 2 large beakers both large beakers are to be filled with hot water (labtutor). Then obtain seven conical tubes these will be used to collect the levels of gas, you will also need test tube a stopper and a plastic tube (labtutor). You want to fill the conical tube to at least 50 ml of water (Cressy). Take the four conical tubes filled with water and place two in each beaker, to do this you must invert the tube and cover the release hole as to not lose any water (Cressy). Then place the beakers with the tubes in the bath so they can be at the same temperature as the bath (Cressy). Next mark all of your test tubes in number order to be sure which tube contains what concentrations and pH (Cressy). Having mixed a solution to the specifications of 2.5 ml of glucose in all tubes, 3 ml of yeast in 2 tubes of pH 5, 2 tubes of pH 9, and the single pH 7 tube, the remaining two tubes will contain no yeast as they will be negative controls. Next add 2 ml of pH buffer 3 tubes will receive pH of 5, three will receive a pH of 9 and a single tube of pH 7. Finally add pure water to make sure all test tubes have 10 ml of solution. When making the solutions