Catalase Lab Report

Enzymes are proteins in living organisms that speed up chemical reactions and are also vital in making sure our bodies can function. These chemical reactions are know as catalysts. However, enzymes can not create these reactions by themselves. So, they work with molecules called substrates to help them out. The enzyme and substrate would usually fit perfectly together, unless the enzyme loses its shape. Two major things that can impact the disfiguration of the enzyme's shape are changes in temperature or pH levels. In this lab, our goal was to show if enzyme activity differs due to changes in temperature and pH levels by using catalase solution (potato puree), hydrogen peroxide, Hydrochloric Acid solution, sodium hydroxide (base

Catalase is an important enzyme that protects cells from oxidative damage, which hydrogen peroxide can cause. It is an incredibly efficient enzyme where one catalase molecule can convert millions of hydrogen peroxide molecules each second.

Students will be observing normal catalase reaction, the effect of temperature on enzyme activity, and the effect of pH on enzyme activity in this experiment. The enzymes will all around perform better when exposed in room temperature than when it is exposed to hot and cold temperatures. This is based on the fact that the higher the temperature, the better the enzymes will perform, but as the temperature reaches a certain high degree, the enzymes will start to denature, or lose their function.

Enzymes are a very important to the biological process. Enzymes help break down food and are essential in helping convert that food to energy. Enzymes have a single function, which makes them unique and need specific conditions in order for the reaction to occur. Every function in an organism has its own unique enzyme (What are enzymes?). One important thing to know about enzymes is that they are proteins. According to rsc.org enzymes are efficient catalysts for biochemical reactions and they, “speed up reactions by providing an alternative reaction pathway of lower activation energy” (Enzymes).

The null hypothesis for the first experiment was that substrate concentration would have no effect on the reaction rate. It was hypothesized that the reaction rate would increase with rising substrate concentrations, until all active sites were bound. The null hypothesis for the second experiment was that temperature would not have an effect on reaction rates. It was hypothesized that until the enzyme is denatured, as temperature increased, so would the reaction rate.

An Enzyme is a protein that in essence speeds up biological reactions. So that would mean that a Catalase is an enzyme reaction that decomposes hydrogen peroxide to water and oxygen. It is primarily found in the liver and it is important in protecting the cell from damaging oxidative reactions.

Enzymes are biological catalysts. They work by lowering the activation energy needed to initiate a chemical reaction. Enzymes work within an optimal temperature and optimal pH. Enzymes are highly specific for a single substrate. The Enzyme is usually much larger in size than the substrate it binds to. In some cases, an enzyme requires something called a cofactor to begin the chemical reaction. There were four different experiments that were executed in the enzyme lab. Experiment 7.1, the first experiment, was performed to test the effect of temperature on enzymatic

Once each test tube had been in the respective temperatures for a minimum of 10 minutes, test tube 1 was taken out of its bath and the rest of the tubes were left in their respective places. To find the rate of enzyme activity for test tube 1, 10 drops of enzyme solution were added, and the timer began. The contents were poured into a clean 250mL Naglene bottle, and swirled to mix. Next, the O2 Gas Sensor

Organisms cannot depend solely on spontaneous reactions for the production of materials because they occur slowly and are not responsive to the organism's needs (Martineau, Dean, et al, Laboratory Manual, 43). In order to speed up the reaction process, cells use enzymes as biological catalysts. Enzymes are able to speed up the reaction through lowering activation energy. Additionally, enzymes facilitate reactions without being consumed (manual,43). Each enzyme acts on a specific molecule or set of molecules referred to as the enzyme's substrate and the results of this reaction are called products (manual 43). As a result, enzymes promote a reaction so that substrates are converted into products on a faster pace (manual 43). Most enzymes are proteins whose structure is determined by its sequence of its amino acids. Enzymes are designed to function the best under physiological conditions of PH and temperature. Any change of these variables that change the conformation of the enzyme will destroy or enhance enzyme activity(manual, 43).

Enzymes are proteins that catalyse chemical reactions by the lowering activation energy and speeding up the rate of reaction. The absence of enzymes would result in substantially slower reactions; life would not be sustainable since chemical reactions required for maintaining life would not occur fast enough, (BBC, 2017).

Introduction Enzymes are found in humans, animals and plants. An enzyme is a protein that acts as a catalyst, which brings out a biochemical reaction. Biochemical reactions are important in humans because they help with many important functions such as digestion and the breaking down of waste in the body. Catalase is an enzyme found in almost all living organisms that are exposed daily to oxygen (such as fruits, vegetables and animals). To determine how Catalase reacts with hydrogen peroxide (a compound also found in the human body) under two different conditions is the focus of the following experiment called the, “Enzyme Experiment.”

This experiment is designed to analyze how the enzyme catalase activity is affected by the pH levels. The experiment has also been designed to outline all of the directions and the ways by which the observation can be made clearly and accurately. Yeast, will be used as the enzyme and hydrogen peroxide will be used as a substrate. This experiment will be used to determine the effects of the concentration of the hydrogen peroxide versus the rate of reaction of the enzyme catalase.

generally act as a catalase that initially bring about a chemical reaction.” Enzymes play an

The objective of the lab was to examine the effects of environmental variables on the functions of an enzyme. To the point, an experiment was conducted to test the effect of pH on the function of the enzyme Amylase.

To study the effects of temperature, pH, enzyme concentration, and substrate concentration there were certain steps that were followed in order to conduct this experiment. Each factor had a separate procedure to follow to find how each had a different effect on the enzyme.